Logo of biochemjBJ Latest papers and much more!
Biochem J. Jul 15, 1988; 253(2): 329–336.
PMCID: PMC1149302

Co-operativity in seminal ribonuclease function. Kinetic studies.


Kinetic studies with substrates of the hydrolytic rate-limiting reaction step revealed that the non-hyperbolic kinetics of bovine seminal RNAse may not be ascribed to microheterogeneity of the enzyme or to hysteretic effects. The substrate saturation curves with intermediate plateau and the activating and inhibiting effects of the reaction product, respectively at low and high concentrations, are explained in terms of mixed co-operativity, with binding at subsites that is a prerequisite for full activity of the enzyme. A model is proposed that is supported also by the results of binding studies.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Andini S, D'Alessio G, Di Donato A, Paolillo L, Piccoli R, Trivellone E. Comparative proton NMR studies of bovine semen and pancreas ribonucleases. Biochim Biophys Acta. 1983 Feb 15;742(3):530–538. [PubMed]
  • Capasso S, Giordano F, Mattia CA, Mazzarella L, Zagari A. Refinement of the structure of bovine seminal ribonuclease. Biopolymers. 1983 Jan;22(1):327–332. [PubMed]
  • Cornish-Bowden A, Koshland DE., Jr Diagnostic uses of the Hill (Logit and Nernst) plots. J Mol Biol. 1975 Jun 25;95(2):201–212. [PubMed]
  • CRESTFIELD AM, STEIN WH, MOORE S. On the aggregation of bovine pancreatic ribonuclease. Arch Biochem Biophys. 1962 Sep;Suppl 1:217–222. [PubMed]
  • D'Alessio G, Floridi A, De Prisco R, Pignero A, Leone E. [Bull semen ribonucleases. 1. Purification and physico-chemical properties of the major component]. Eur J Biochem. 1972 Mar 27;26(2):153–161. [PubMed]
  • D'Alessio G, Malorni MC, Parente A. Dissociation of bovine seminal ribonuclease into catalytically active monomers by selective reduction and alkylation of the intersubunit disulfide bridges. Biochemistry. 1975 Mar 25;14(6):1116–1122. [PubMed]
  • Di Donato A, D'Alessio G. Heterogeneity of bovine seminal ribonuclease. Biochemistry. 1981 Dec 8;20(25):7232–7237. [PubMed]
  • Di Donato A, Galletti P, D'Alessio G. Selective deamidation and enzymatic methylation of seminal ribonuclease. Biochemistry. 1986 Dec 30;25(26):8361–8368. [PubMed]
  • Di Donato A, Piccoli R, D'Alessio G. Co-operativity in seminal ribonuclease function: binding studies. Biochem J. 1987 Jan 15;241(2):435–440. [PMC free article] [PubMed]
  • Donadio S, Tamburrini M, Di Donato A, Piccoli R, D'Alessio G. Site-directed alkylation and site-site interactions in bovine seminal ribonuclease. Eur J Biochem. 1986 Jun 16;157(3):475–480. [PubMed]
  • Floridi A, D'Alessio G, Leone E. Bull semen ribonucleases. 2. Catalytic properties of the major component. Eur J Biochem. 1972 Mar 27;26(2):162–167. [PubMed]
  • HERRIES DG, MATHIAS AP, RABIN BR. The active site and mechanism of action of bovine pancreatic ribonuclease. 3. The pH-dependence of the kinetic parameters for the hydrolysis of cytidine 2',3'-phosphate. Biochem J. 1962 Oct;85:127–134. [PMC free article] [PubMed]
  • Libonati M, Floridi A. Breakdown of double-stranded RNA by bull semen ribonuclease. Eur J Biochem. 1969 Mar;8(1):81–87. [PubMed]
  • Matthew JB, Richards FM. Anion binding and pH-dependent electrostatic effects in ribonuclease. Biochemistry. 1982 Sep 28;21(20):4989–4999. [PubMed]
  • Neet KE. Cooperativity in enzyme function: equilibrium and kinetic aspects. Methods Enzymol. 1980;64:139–192. [PubMed]
  • Neet KE, Ainslie GR., Jr Hysteretic enzymes. Methods Enzymol. 1980;64:192–226. [PubMed]
  • Parés X, Llorens R, Arús C, Cuchillo CM. The reaction of bovine pancreatic ribonuclease A with 6-chloropurineriboside 5'-monophosphate. Evidence on the existence of a phosphate-binding sub-site. Eur J Biochem. 1980 Apr;105(3):571–579. [PubMed]
  • Piccoli R, D'Alessio G. Relationships between nonhyperbolic kinetics and dimeric structure in ribonucleases. J Biol Chem. 1984 Jan 25;259(2):693–695. [PubMed]
  • Piccoli R, Di Donato A, Dudkin S, D'Alessio G. Bovine seminal ribonuclease: non-hyperbolic kinetics in the second reaction strep. FEBS Lett. 1982 Apr 19;140(2):307–310. [PubMed]
  • Suzuki H, Parente A, Farina B, Greco L, La Montagna R, Leone E. Complete amino-acid sequence of bovine seminal ribonuclease, a dimeric protein from seminal plasma. Biol Chem Hoppe Seyler. 1987 Oct;368(10):1305–1312. [PubMed]
  • Tamburrini M, Piccoli R, De Prisco R, Di Donato A, D'Alessio G. Fast and high-yielding procedures for the isolation of bovine seminal RNAase. Ital J Biochem. 1986 Jan-Feb;35(1):22–32. [PubMed]
  • Taniguchi T, Libonati M. Action of ribonuclease BS-1 on a DNA-RNA hybrid. Biochem Biophys Res Commun. 1974 May 7;58(1):280–286. [PubMed]
  • Walker EJ, Ralston GB, Darvey IG. An allosteric model for ribonuclease. Biochem J. 1975 Jun;147(3):425–433. [PMC free article] [PubMed]
  • Walker EJ, Ralston GB, Darvey IG. Further evidence for an allosteric model for ribonuclease. Biochem J. 1976 Feb 1;153(2):329–337. [PMC free article] [PubMed]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • Cited in Books
    Cited in Books
    PubMed Central articles cited in books
  • Compound
    PubChem Compound links
  • MedGen
    Related information in MedGen
  • PubMed
    PubMed citations for these articles
  • Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...