• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of biochemjBJ Latest papers and much more!
Biochem J. Nov 15, 1987; 248(1): 167–171.
PMCID: PMC1148514

Biotin binding to avidin. Oligosaccharide side chain not required for ligand association.

Abstract

A commercially available, purified preparation of avidin was found to comprise two polypeptide bands (Mr 18,000 and Mr 15,500 respectively). Both bands bound biotin as assessed by biotin overlays of protein blots. The Mr 15,500 polypeptide was found to differ from the Mr 18,000 polypeptide only in its sugar content. When the commercial preparation was applied to a concanavalin A affinity column, the glycosylated forms were retarded as expected, and homotypic nonglycosylated avidin tetramers which failed to bind selectively to the column were collected in the effluent. The biotin-binding properties of the nonglycosylated avidin were equivalent to those obtained for the native (glycosylated) avidin molecule, indicating that the oligosaccharide moiety is not essential for the binding activity.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.4M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bayer EA, Wilchek M. The use of the avidin-biotin complex as a tool in molecular biology. Methods Biochem Anal. 1980;26:1–45. [PubMed]
  • Bayer EA, Ben-Hur H, Wilchek M. A sensitive enzyme assay for biotin, avidin, and streptavidin. Anal Biochem. 1986 Apr;154(1):367–370. [PubMed]
  • Bruch RC, White HB., 3rd Compositional and structural heterogeneity of avidin glycopeptides. Biochemistry. 1982 Oct 12;21(21):5334–5341. [PubMed]
  • CHAIET L, WOLF FJ. THE PROPERTIES OF STREPTAVIDIN, A BIOTIN-BINDING PROTEIN PRODUCED BY STREPTOMYCETES. Arch Biochem Biophys. 1964 Jul 20;106:1–5. [PubMed]
  • Clegg JC. Glycoprotein detection in nitrocellulose transfers of electrophoretically separated protein mixtures using concanavalin A and peroxidase: application to arenavirus and flavivirus proteins. Anal Biochem. 1982 Dec;127(2):389–394. [PubMed]
  • DeLange RJ. Egg white avidin. I. Amino acid composition; sequence of the amino- and carboxyl-terminal cyanogen bromide peptides. J Biol Chem. 1970 Mar 10;245(5):907–916. [PubMed]
  • DeLange RJ, Huang TS. Egg white avidin. 3. Sequence of the 78-residue middle cyanogen bromide peptide. Complete amino acid sequence of the protein subunit. J Biol Chem. 1971 Feb 10;246(3):698–709. [PubMed]
  • Gershoni JM, Palade GE. Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to a positively charged membrane filter. Anal Biochem. 1982 Aug;124(2):396–405. [PubMed]
  • Gershoni JM, Bayer EA, Wilchek M. Blot analyses of glycoconjugates: enzyme-hydrazide--a novel reagent for the detection of aldehydes. Anal Biochem. 1985 Apr;146(1):59–63. [PubMed]
  • Gershoni JM, Davis FE, Palade GE. Protein blotting in uniform or gradient electric fields. Anal Biochem. 1985 Jan;144(1):32–40. [PubMed]
  • GREEN NM. AVIDIN. 1. THE USE OF (14-C)BIOTIN FOR KINETIC STUDIES AND FOR ASSAY. Biochem J. 1963 Dec;89:585–591. [PMC free article] [PubMed]
  • Green NM. Avidin. Adv Protein Chem. 1975;29:85–133. [PubMed]
  • Green NM, Toms EJ. The properties of subunits of avidin coupled to sepharose. Biochem J. 1973 Aug;133(4):687–700. [PMC free article] [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • REISFELD RA, LEWIS UJ, WILLIAMS DE. Disk electrophoresis of basic proteins and peptides on polyacrylamide gels. Nature. 1962 Jul 21;195:281–283. [PubMed]
  • RONDLE CJ, MORGAN WT. The determination of glucosamine and galactosamine. Biochem J. 1955 Dec;61(4):586–589. [PMC free article] [PubMed]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Compound
    Compound
    PubChem Compound links
  • MedGen
    MedGen
    Related information in MedGen
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...