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Biochem J. May 15, 1985; 228(1): 269–272.
PMCID: PMC1144979

'Non-specific' binding. The problem, and a solution.

Abstract

The concept of 'non-specific' binding, as it relates to studies of the binding of hormones to their receptors, is reviewed. It is concluded that the most widely used operational definition, namely binding that is not displaceable by an excess of unlabelled ligand, is often inaccurate, resulting either in overestimation of the number of high-affinity receptors and underestimation of the affinity of a given hormone for its receptor, or in a curvilinear Scatchard plot suggesting (artifactually) the presence of negative co-operativity or multiple classes of binding sites. The general use of an alternative approach to non-specific binding, in which the non-specific component is assessed from an analysis of total binding, is advocated. The superiority of this approach is illustrated with data on the binding of high-density lipoproteins to their receptors.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Almon RR, Appel SH. Cholinergic sites in skeletal muscle. I. Denervation effects. Biochemistry. 1976 Aug 24;15(17):3662–3667. [PubMed]
  • Cuatrecasas P, Hollenberg MD. Binding of insulin and other hormones to non-receptor materials: saturability, specificity and apparent "negative cooperativity". Biochem Biophys Res Commun. 1975 Jan 6;62(1):31–41. [PubMed]
  • Dana SE, Brown MS, Goldstein JL. Specific, saturable, and high affinity binding of 125I-low density lipoprotein to glass beads. Biochem Biophys Res Commun. 1977 Feb 21;74(4):1369–1376. [PubMed]
  • Klotz IM. Numbers of receptor sites from Scatchard graphs: facts and fantasies. Science. 1982 Sep 24;217(4566):1247–1249. [PubMed]
  • Mendel CM, Almon RR. Associations of [3H]dihydroalprenolol with biological membranes. Gen Pharmacol. 1979;10(1):31–40. [PubMed]
  • Munson PJ, Rodbard D. Ligand: a versatile computerized approach for characterization of ligand-binding systems. Anal Biochem. 1980 Sep 1;107(1):220–239. [PubMed]
  • Munson PJ, Rodbard D. Number of receptor sites from Scatchard and Klotz graphs: a constructive critique. Science. 1983 May 27;220(4600):979–981. [PubMed]
  • Phillips JL. "Specific" binding of radioiodinated transferrin to polypropylene culture tubes. Biochem Biophys Res Commun. 1976 Aug 9;71(3):726–732. [PubMed]

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