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Biochem J. May 1, 1985; 227(3): 753–757.
PMCID: PMC1144902

Membrane-bound lactate dehydrogenases and mandelate dehydrogenases of Acinetobacter calcoaceticus. Location and regulation of expression.


Acinetobacter calcoaceticus possesses an L(+)-lactate dehydrogenase and a D(-)-lactate dehydrogenase. Results of experiments in which enzyme activities were measured after growth of bacteria in different media indicated that the two enzymes were co-ordinately induced by either enantiomer of lactate but not by pyruvate, and repressed by succinate or L-glutamate. The two lactate dehydrogenases have very similar properties to L(+)-mandelate dehydrogenase and D(-)-mandelate dehydrogenase. All four enzymes are NAD(P)-independent and were found to be integral components of the cytoplasmic membrane. The enzymes could be solubilized in active form by detergents; Triton X-100 or Lubrol PX were particularly effective D(-)-Lactate dehydrogenase and D(-)-mandelate dehydrogenase could be selectively solubilized by the ionic detergents cholate, deoxycholate and sodium dodecyl sulphate.

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Selected References

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  • Borthwick AC, Holms WH, Nimmo HG. Isolation of active and inactive forms of isocitrate dehydrogenase from Escherichia coli ML 308. Eur J Biochem. 1984 Jun 1;141(2):393–400. [PubMed]
  • Futai M. Membrane D-lactate dehydrogenase from Escherichia coli. Purification and properties. Biochemistry. 1973 Jun 19;12(13):2468–2474. [PubMed]
  • Futai M, Kimura H. Inducible membrane-bound L-lactate dehydrogenase from Escherichia coli. Purification and properties. J Biol Chem. 1977 Aug 25;252(16):5820–5827. [PubMed]
  • Garvie EI. Bacterial lactate dehydrogenases. Microbiol Rev. 1980 Mar;44(1):106–139. [PMC free article] [PubMed]
  • Hegeman GD. Synthesis of the enzymes of the mandelate pathway by Pseudomonas putida. I. Synthesis of enzymes by the wild type. J Bacteriol. 1966 Mar;91(3):1140–1154. [PMC free article] [PubMed]
  • Hills CA, Fewson CA. Mutant strains of Acinetobacter calcoaceticus possessing additional mandelate dehydrogenases. Identification and preliminary characterization of the enzymes. Biochem J. 1983 Feb 1;209(2):379–386. [PMC free article] [PubMed]
  • Hills CA, Fewson CA. Regulation of expression of novel mandelate dehydrogenases in mutants of Acinetobacter calcoaceticus. J Gen Microbiol. 1983 Jul;129(7):2009–2015. [PubMed]
  • Kennedy SI, Fewson CA. Enzymes of the mandelate pathway in Bacterium N.C.I.B. 8250. Biochem J. 1968 Apr;107(4):497–506. [PMC free article] [PubMed]
  • Kline ES, Mahler HR. The lactic dehydrogenases of E. coli. Ann N Y Acad Sci. 1965 Jul 31;119(3):905–919. [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • O'Donnell MJ, Fewson CA. Solubilization and stabilization of L(+)-mandelate dehydrogenase from Acinetobacter calcoaceticus. Biochem Soc Trans. 1980 Oct;8(5):653–654. [PubMed]
  • Olson ST, Massey V. Purification and properties of the flavoenzyme D-lactate dehydrogenase from Megasphaera elsdenii. Biochemistry. 1979 Oct 16;18(21):4714–4724. [PubMed]
  • Pratt EA, Fung LW, Flowers JA, Ho C. Membrane-bound D-lactate dehydrogenase from Escherichia coli: purification and properties. Biochemistry. 1979 Jan 23;18(2):312–316. [PubMed]
  • Scott CC, Makula SR, Finnerty WR. Isolation and characterization of membranes from a hydrocarbon-oxidizing Acinetobacter sp. J Bacteriol. 1976 Jul;127(1):469–480. [PMC free article] [PubMed]
  • STANIER RY, GUNSALUS IC, GUNSALUS CF. The enzymatic conversion of mandelic acid to benzoic acid. II. Properties of the particulate fractions. J Bacteriol. 1953 Nov;66(5):543–547. [PMC free article] [PubMed]

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