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Biochem J. Apr 15, 1985; 227(2): 545–554.
PMCID: PMC1144874

Type X collagen, a product of hypertrophic chondrocytes.

Abstract

The synthesis of collagen types IX and X by explants of chick-embryo cartilages was investigated. When sternal cartilage labelled for 24h with [3H]proline was extracted with 4M-guanidinium chloride, up to 20% of the 3H-labelled collagen laid down in the tissue could be accounted for by the low-Mr collagenous polypeptides (H and J chains) of type IX collagen; but no type X collagen could be detected. Explants of tibiotarsal and femoral cartilages were found to synthesize type IX collagen mainly in zones 1 and 2 of chondrocyte proliferation and elongation, whereas type X collagen was shown to be a product of the hypertrophic chondrocytes in zone 3. Pulse-chase experiments with tibiotarsal (zone-3) explants demonstrated a time-dependent conversion of type X procollagen into a smaller species whose polypeptides were of Mr 49 000. The processed chains [alpha 1(X) chains] were shown by peptide mapping techniques to share a common identity with the pro alpha 1(X) chains of Mr 59 000. No evidence for processing of type IX collagen was obtained in analogous pulse-chase experiments with sternal tissue. When chondrocytes from tibiotarsal cartilage (zone 3) were cultured on plastic under standard conditions for 4-10 weeks they released large amounts of type X procollagen into the medium. However, 2M-MgCl2 extracts of the cell layer were found to contain mainly the processed collagen comprising alpha 1(X) chains. The native type X procollagen purified from culture medium was shown by rotary shadowing to occur as a short rod-like molecule 148 nm in length with a terminal globular extension, whereas the processed species comprising alpha 1(X) chains of Mr 49 000 was detected by electron microscopy as the linear 148 nm segment.

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  • Ayad S, Abedin MZ, Grundy SM, Weiss JB. Isolation and characterisation of an unusual collagen from hyaline cartilage and intervertebral disc. FEBS Lett. 1981 Jan 26;123(2):195–199. [PubMed]
  • Ayad S, Abedin MZ, Weiss JB, Grundy SM. Characterisation of another short-chain disulphide-bonded collagen from cartilage, vitreous and intervertebral disc. FEBS Lett. 1982 Mar 22;139(2):300–304. [PubMed]
  • Bonner WM, Laskey RA. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. [PubMed]
  • Bruckner P, Mayne R, Tuderman L. p-HMW-collagen, a minor collagen obtained from chick embryo cartilage without proteolytic treatment of the tissue. Eur J Biochem. 1983 Nov 2;136(2):333–339. [PubMed]
  • Burgeson RE, Hollister DW. Collagen heterogeneity in human cartilage: identification of several new collagen chains. Biochem Biophys Res Commun. 1979 Apr 27;87(4):1124–1131. [PubMed]
  • Capasso O, Gionti E, Pontarelli G, Ambesi-Impiombato FS, Nitsch L, Tajana G, Cancedda R. The culture of chick embryo chondrocytes and the control of their differentiated functions in vitro. I. Characterization of the chondrocyte-specific phenotypes. Exp Cell Res. 1982 Nov;142(1):197–206. [PubMed]
  • Capasso O, Quarto N, Descalzi-Cancedda F, Cancedda R. The low molecular weight collagen synthesized by chick tibial chondrocytes is deposited in the extracellular matrix both in culture and in vivo. EMBO J. 1984 Apr;3(4):823–827. [PMC free article] [PubMed]
  • Duance VC, Wotton SF, Voyle CA, Bailey AJ. Isolation and characterization of the precursor of type M collagen. Biochem J. 1984 Aug 1;221(3):885–889. [PMC free article] [PubMed]
  • Gibson GJ, Schor SL, Grant ME. Effects of matrix macromolecules on chondrocyte gene expression: synthesis of a low molecular weight collagen species by cells cultured within collagen gels. J Cell Biol. 1982 Jun;93(3):767–774. [PMC free article] [PubMed]
  • Gibson GJ, Kielty CM, Garner C, Schor SL, Grant ME. Identification and partial characterization of three low-molecular-weight collagenous polypeptides synthesized by chondrocytes cultured within collagen gels in the absence and in the presence of fibronectin. Biochem J. 1983 May 1;211(2):417–426. [PMC free article] [PubMed]
  • Gibson GJ, Beaumont BW, Flint MH. Synthesis of a low molecular weight collagen by chondrocytes from the presumptive calcification region of the embryonic chick sterna: the influence of culture with collagen gels. J Cell Biol. 1984 Jul;99(1 Pt 1):208–216. [PMC free article] [PubMed]
  • Kielty CM, Hulmes DJ, Schor SL, Grant ME. Embryonic chick cartilage collagens. Differences in the low-Mr species present in sternal cartilage and tibiotarsal articular cartilage. FEBS Lett. 1984 Apr 24;169(2):179–184. [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Laskey RA, Mills AD. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem. 1975 Aug 15;56(2):335–341. [PubMed]
  • Miller EJ. Biochemical characteristics and biological significance of the genetically-distinct collagens. Mol Cell Biochem. 1976 Dec 10;13(3):165–192. [PubMed]
  • Reese CA, Mayne R. Minor collagens of chicken hyaline cartilage. Biochemistry. 1981 Sep 15;20(19):5443–5448. [PubMed]
  • Remington MC, Bashey RI, Brighton CT, Jimenez SA. Biosynthesis of a low molecular weight collagen by rabbit growth plate cartilage organ cultures. Coll Relat Res. 1983 May;3(3):271–277. [PubMed]
  • Ricard-Blum S, Hartmann DJ, Herbage D, Payen-Meyran C, Ville G. Biochemical properties and immunolocalization of minor collagens in foetal calf cartilage. FEBS Lett. 1982 Sep 20;146(2):343–347. [PubMed]
  • Schmid TM, Conrad HE. A unique low molecular weight collagen secreted by cultured chick embryo chondrocytes. J Biol Chem. 1982 Oct 25;257(20):12444–12450. [PubMed]
  • Schmid TM, Conrad HE. Metabolism of low molecular weight collagen by chondrocytes obtained from histologically distinct zones of the chick embryo tibiotarsus. J Biol Chem. 1982 Oct 25;257(20):12451–12457. [PubMed]
  • Schmid TM, Linsenmayer TF. A short chain (pro)collagen from aged endochondral chondrocytes. Biochemical characterization. J Biol Chem. 1983 Aug 10;258(15):9504–9509. [PubMed]
  • Schmid TM, Mayne R, Bruns RR, Linsenmayer TF. Molecular structure of short-chain (SC) cartilage collagen by electron microscopy. J Ultrastruct Res. 1984 Feb;86(2):186–191. [PubMed]
  • Shimokomaki M, Duance VC, Bailey AJ. Identification of a new disulphide bonded collagen from cartilage. FEBS Lett. 1980 Nov 17;121(1):51–54. [PubMed]
  • Shimokomaki M, Duance VC, Bailey AJ. Identification of two further collagenous fractions from articular cartilage. Biosci Rep. 1981 Jul;1(7):561–570. [PubMed]
  • Stocum DL, Davis RM, Leger M, Conrad HE. Development of the tibiotarsus in the chick embryo: biosynthetic activities of histologically distinct regions. J Embryol Exp Morphol. 1979 Dec;54:155–170. [PubMed]
  • von der Mark K, van Menxel M, Wiedemann H. Isolation and characterization of new collagens from chick cartilage. Eur J Biochem. 1982 May;124(1):57–62. [PubMed]
  • von der Mark K, van Menxel M, Wiedemann H. Isolation and characterization of a precursor form of M collagen from embryonic chicken cartilage. Eur J Biochem. 1984 Feb 1;138(3):629–633. [PubMed]

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