• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of biochemjBJ Latest papers and much more!
Biochem J. Sep 1, 1984; 222(2): 379–387.
PMCID: PMC1144189

Stimulation of mitochondrial pyruvate metabolism and citrulline synthesis by dexamethasone. Effect of isolation and incubation media.

Abstract

Hepatic mitochondria isolated in 0.3 M-sucrose or 0.3 M-mannitol from rats treated for 3h with dexamethasone displayed stimulated rates of pyruvate carboxylation and decarboxylation and citrulline synthesis when compared with organelles from control animals. Mitochondria isolated in mannitol also displayed elevated rates of pyruvate carboxylation and decarboxylation when compared with those isolated in sucrose, and this stimulation was shown to be independent of the lengthy isolation procedure. Citrulline synthesis proceeded at similar rates in mitochondria isolated in either sugar. The concentration of exchangeable adenine nucleotides was identical in mitochondria isolated in sucrose or mannitol, suggesting that those prepared in the former sugar are not more permeable to metabolites than those prepared in the latter. The matrix volume of mitochondria isolated in mannitol was greater than that of mitochondria isolated in sucrose, and the effect of mannitol on pyruvate metabolism was mimicked by swelling the organelles in hypo-osmotic sucrose. Measurements of the extra-matrix volume by using [14C]sucrose or [14C]mannitol suggest that mannitol can permeate mitochondria to a greater extent than can sucrose. The possibility that mannitol elicits its effect by entering the mitochondrial matrix and so initiating swelling is discussed.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.3M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Adam PA, Haynes RC., Jr Control of hepatic mitochondrial CO2 fixation by glucagon, epinephrine, and cortisol. J Biol Chem. 1969 Dec 10;244(23):6444–6450. [PubMed]
  • Allan EH, Titheradge MA. Effect of treatment of rats with dexamethasone in vivo on gluconeogenesis and metabolite compartmentation in subsequently isolated hepatocytes. Biochem J. 1984 Apr 1;219(1):117–123. [PMC free article] [PubMed]
  • Allan EH, Chisholm AB, Titheradge MA. Hormonal stimulation of mitochondrial pyruvate carboxylation in filipin-treated hepatocytes. Biochem J. 1983 May 15;212(2):417–426. [PMC free article] [PubMed]
  • Armston AE, Halestrap AP, Scott RD. The nature of the changes in liver mitochondrial function induced by glucagon treatment of rats. The effects of intramitochondrial volume, aging and benzyl alcohol. Biochim Biophys Acta. 1982 Sep 15;681(3):429–439. [PubMed]
  • Bryla J, Harris EJ, Plumb JA. The stimulatory effect of glucagon and dibutyryl cyclic AMP on ureogenesis and gluconeogenesis in relation to the mitochondrial ATP content. FEBS Lett. 1977 Aug 15;80(2):443–448. [PubMed]
  • CHANCE B, WILLIAMS GR. The respiratory chain and oxidative phosphorylation. Adv Enzymol Relat Subj Biochem. 1956;17:65–134. [PubMed]
  • Chisholm AB, Allan EH, Titheradge MA. Regulation of mitochondrial pyruvate carboxylation in isolated hepatocytes by acute insulin treatment. Biochem J. 1983 Aug 15;214(2):451–458. [PMC free article] [PubMed]
  • Corvera S, García-Sáinz JA. Vasopressin and angiotensin II stimulate ureogenesis through increased mitochondrial citrulline production. Life Sci. 1982 Nov 29;31(22):2493–2498. [PubMed]
  • Garrison JC, Borland MK. Regulation of mitochondrial pyruvate carboxylation and gluconeogenesis in rat hepatocytes via an alpha-adrenergic, adenosine 3':5'-monophosphate-independent mechanism. J Biol Chem. 1979 Feb 25;254(4):1129–1133. [PubMed]
  • Garrison JC, Haynes RC., Jr The hormonal control of gluconeogenesis by regulation of mitochondrial pyruvate carboxylation in isolated rat liver cells. J Biol Chem. 1975 Apr 25;250(8):2769–2777. [PubMed]
  • Halestrap AP. Stimulation of pyruvate transport in metabolizing mitochondria through changes in the transmembrane pH gradient induced by glucagon treatment of rats. Biochem J. 1978 Jun 15;172(3):389–398. [PMC free article] [PubMed]
  • Halestrap AP, Denton RM. Specific inhibition of pyruvate transport in rat liver mitochondria and human erythrocytes by alpha-cyano-4-hydroxycinnamate. Biochem J. 1974 Feb;138(2):313–316. [PMC free article] [PubMed]
  • Halestrap AP, Quinlan PT. The intramitochondrial volume measured using sucrose as an extramitochondrial marker overestimates the true matrix volume determined with mannitol. Biochem J. 1983 Aug 15;214(2):387–393. [PMC free article] [PubMed]
  • Haynes RC, Jr, Garrison JC, Yamazaki RK. Comparison of effects of glucagon and valinomycin on rat liver mitochondria and cells. Mol Pharmacol. 1974 May;10(3):381–388. [PubMed]
  • Hensgens HE, Verhoeven AJ, Meijer AJ. The relationship between intramitochondrial N-acetylglutamate and activity of carbamoyl-phosphate synthetase (ammonia). The effect of glucagon. Eur J Biochem. 1980;107(1):197–205. [PubMed]
  • Hunter DR, Haworth RA, Southard JH. Relationship between configuration, function, and permeability in calcium-treated mitochondria. J Biol Chem. 1976 Aug 25;251(16):5069–5077. [PubMed]
  • Jensen CB, Sistare FD, Hamman HC, Haynes RC., Jr Stimulation of mitochondrial functions by glucagon treatment. Evidence that effects are not artifacts of mitochondrial isolation. Biochem J. 1983 Mar 15;210(3):819–827. [PMC free article] [PubMed]
  • JOHNSON D, LARDY H. Substrate-selective inhibition of mitochondrial oxidations by enhanced tonicity. Nature. 1958 Mar 8;181(4610):701–702. [PubMed]
  • Lacey JH, Bradford NM, Joseph SK, McGivan JD. Increased activity of phosphate-dependent glutaminase in liver mitochondria as a result of glucagon treatment of rats. Biochem J. 1981 Jan 15;194(1):29–33. [PMC free article] [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • Martin AD, Allan EH, Titheradge MA. The stimulation of mitochondrial pyruvate carboxylation after dexamethasone treatment of rats. Biochem J. 1984 Apr 1;219(1):107–115. [PMC free article] [PubMed]
  • Meijer AJ, van Woerkom GM. Control of the rate of citrulline synthesis by short-term changes in N-acetylglutamate levels in isolated rat-liver mitochondria. FEBS Lett. 1978 Feb 1;86(1):117–121. [PubMed]
  • Mörikofer-Zwez S, Kunin AS, Walter P. Effects of calcium and sucrose on pyruvate carboxylase activity in intact rat liver mitochondria. J Biol Chem. 1973 Nov 10;248(21):7588–7594. [PubMed]
  • Nicholls DG, Lindberg O. Inhibited respiration and ATPase activity of rat liver mitochondria under conditions of matrix condensation. FEBS Lett. 1972 Sep 1;25(1):61–64. [PubMed]
  • Quinlan PT, Thomas AP, Armston AE, Halestrap AP. Measurement of the intramitochondrial volume in hepatocytes without cell disruption and its elevation by hormones and valinomycin. Biochem J. 1983 Aug 15;214(2):395–404. [PMC free article] [PubMed]
  • Rabier D, Briand P, Petit F, Parvy P, Kamoun P, Cathelineau L. Acute effects of glucagon on citrulline biosynthesis. Biochem J. 1982 Sep 15;206(3):627–631. [PMC free article] [PubMed]
  • Sambasivarao D, Sitaramam V. Studies on the non-linear osmotic pressure-volume relationship in mitochondria and entry of sucrose into the matrix space during centrifugation. Biochim Biophys Acta. 1983 Feb 17;722(2):256–270. [PubMed]
  • Siess EA. Influence of isolation media on the preservation of mitochondrial functions. Hoppe Seylers Z Physiol Chem. 1983 Mar;364(3):279–289. [PubMed]
  • Siess EA. Different actions of mono- and disaccharides on rat liver mitochondria. Hoppe Seylers Z Physiol Chem. 1983 Jul;364(7):835–838. [PubMed]
  • Siess EA, Brocks DG, Lattke HK, Wieland OH. Effect of glucagon on metabolite compartmentation in isolated rat liver cells during gluconeogenesis from lactate. Biochem J. 1977 Aug 15;166(2):225–235. [PMC free article] [PubMed]
  • Siess EA, Fahimi FM, Wieland OH. Evidence that glucagon stabilizes rather than activates mitochondrial functions in rat liver. Hoppe Seylers Z Physiol Chem. 1981 Dec;362(12):1643–1651. [PubMed]
  • Sitaramam V, Sarma MK. Gravitational field enhances permeability of biological membranes to sucrose: an experimental refutation of sucrose-space hypothesis. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3441–3445. [PMC free article] [PubMed]
  • Stewart PM, Walser M. Short term regulation of ureagenesis. J Biol Chem. 1980 Jun 10;255(11):5270–5280. [PubMed]
  • Stucki JW, Brawand F, Walter P. Regulation of pyruvate metabolim in rat-liver mitochondria by adenine nucleotides and fatty acids. Eur J Biochem. 1972 May;27(1):181–191. [PubMed]
  • Thomas AP, Halestrap AP. Computer stimulation of the effects of alpha-cyano-4-hydroxycinnamate on gluconeogenesis from L-lactate in rat liver cells. Biochem J. 1981 Sep 15;198(3):561–564. [PMC free article] [PubMed]
  • Titheradge MA, Coore HG. The mitochondrial pyruvate carrier, its exchange properties and its regulation by glucagon. FEBS Lett. 1976 Mar 15;63(1):45–50. [PubMed]
  • Titheradge MA, Coore HG. Hormonal regulation of liver mitochondrial pyruvate carrier in relation to gluconeogenesis and lipogenesis. FEBS Lett. 1976 Nov 15;72(1):73–78. [PubMed]
  • Titheradge MA, Haynes RC., Jr Glucagon treatment stimulates the oxidation of durohydroquinone by rat liver mitochondria. FEBS Lett. 1979 Oct 15;106(2):330–334. [PubMed]
  • Titheradge MA, Haynes RC., Jr The hormonal stimulation of ureogenesis in isolated hepatocytes through increases in mitochondrial ATP production. Arch Biochem Biophys. 1980 Apr 15;201(1):44–55. [PubMed]
  • Triebwasser KC, Freedland RA. The effect of glucagon on ureagenesis from ammonia by isolated rat hepatocytes. Biochem Biophys Res Commun. 1977 Jun 20;76(4):1159–1165. [PubMed]
  • Verhoeven AJ, Hensgens HE, Meijer AJ, Tager JM. On the nature of the stimulation by glucagon of citrulline synthesis in rat-liver mitochondria. FEBS Lett. 1982 Apr 19;140(2):270–272. [PubMed]
  • von Glutz G, Walter P. Regulation of pyruvate carboxylation by acetyl-CoA in rat liver mitochondria. FEBS Lett. 1976 Dec 31;72(2):299–303. [PubMed]
  • Wakat DK, Haynes RC., Jr Glucocorticoid-stimulated utilization of substrates in hepatic mitochondria. Arch Biochem Biophys. 1977 Dec;184(2):561–571. [PubMed]
  • Wanders RJ, Van Woerkom GM, Nooteboom RF, Meijer AJ, Tager JM. Relationship between the rate of citrulline synthesis and bulk changes in the intramitochondrial ATP/ADP ratio in rat-liver mitochondria. Eur J Biochem. 1981 Jan;113(2):295–302. [PubMed]
  • Yamazaki RK. Glucagon stimulation of mitochondrial respiration. J Biol Chem. 1975 Oct 10;250(19):7924–7930. [PubMed]
  • Yamazaki RK, Graetz GS. Glucagon stimulation of citrulline formation in isolated hepatic mitochondria. Arch Biochem Biophys. 1977 Jan 15;178(1):19–25. [PubMed]
  • Yamazaki RK, Haynes RC., Jr Dissociation of pyruvate dehydrogenase from the glucagon stimulation of pyruvate carboxylation in rat liver mitochondria. Arch Biochem Biophys. 1975 Feb;166(2):575–583. [PubMed]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

Links

  • Compound
    Compound
    PubChem Compound links
  • MedGen
    MedGen
    Related information in MedGen
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...