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J Clin Pathol. Dec 1987; 40(12): 1405–1412.
PMCID: PMC1141274

Monoclonal antibody L26: an antibody that is reactive with normal and neoplastic B lymphocytes in routinely fixed and paraffin wax embedded tissues.

Abstract

Formalin fixed and paraffin wax embedded tissue from 85 well characterised cases of non-Hodgkin's lymphoma and Hodgkin's disease were studied using the avidin-biotin-peroxidase complex technique. Among the non-Hodgkin's lymphomas all cases of B cell lymphoma were reactive with L26, a monoclonal antibody which is as yet an unclustered pan B cell reagent, with the exception of pre-B cell acute lymphoblastic leukaemia and malignant lymphoma plasmacytic. Eighteen well characterised cases of T cell lymphoma, selected to include tumours previously shown to exhibit cross reactivity with antibodies to fixation resistant B cell related antigens, were similarly studied. Neoplastic cells in all but one case were unstained by L26. Twenty seven cases of Hodgkin's disease were also examined. In five cases all Reed-Sternberg cells and their variants were strongly stained by L26; only a proportion of Reed-Sternberg cells and their variants were recognised in a further five cases. Monoclonal antibody L26 promises to be a valuable reagent for the diagnosis of malignant lymphoma in routinely fixed and paraffin wax embedded tissues. Its advantage lies in its sensitivity and greater B cell specificity than any of the B cell related reagents currently available for the study of malignant lymphoma in fixed tissues.

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Selected References

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  • Dalchau R, Fabre JW. Identification with a monoclonal antibody of a predominantly B lymphocyte-specific determinant of the human leukocyte common antigen. Evidence for structural and possible functional diversity of the human leukocyte common molecule. J Exp Med. 1981 Apr 1;153(4):753–765. [PMC free article] [PubMed]
  • West KP, Warford A, Fray L, Allen M, Campbell AC, Lauder I. The demonstration of B-cell, T-cell and myeloid antigens in paraffin sections. J Pathol. 1986 Oct;150(2):89–101. [PubMed]
  • Epstein AL, Marder RJ, Winter JN, Fox RI. Two new monoclonal antibodies (LN-1, LN-2) reactive in B5 formalin-fixed, paraffin-embedded tissues with follicular center and mantle zone human B lymphocytes and derived tumors. J Immunol. 1984 Aug;133(2):1028–1036. [PubMed]
  • Hall PA, D'Ardenne AJ, Butler MG, Habeshaw JR, Stansfeld AG. New marker of B lymphocytes, MB2: comparison with other lymphocyte subset markers active in conventionally processed tissue sections. J Clin Pathol. 1987 Feb;40(2):151–156. [PMC free article] [PubMed]
  • Epenetos AA, Bobrow LG, Adams TE, Collins CM, Isaacson PG, Bodmer WF. A monoclonal antibody that detects HLA-D region antigen in routinely fixed, wax embedded sections of normal and neoplastic lymphoid tissues. J Clin Pathol. 1985 Jan;38(1):12–17. [PMC free article] [PubMed]
  • Marder RJ, Variakojis D, Silver J, Epstein AL. Immunohistochemical analysis of human lymphomas with monoclonal antibodies to B cell and Ia antigens reactive in paraffin sections. Lab Invest. 1985 May;52(5):497–504. [PubMed]
  • Salter DM, Krajewski AS, Dewar AE. Immunohistochemical staining of non-Hodgkin's lymphoma with monoclonal antibodies specific for the leucocyte common antigen. J Pathol. 1985 Aug;146(4):345–353. [PubMed]
  • Norton AJ, Isaacson PG. Detailed phenotypic analysis of B-cell lymphoma using a panel of antibodies reactive in routinely fixed wax-embedded tissue. Am J Pathol. 1987 Aug;128(2):225–240. [PMC free article] [PubMed]
  • Ishii Y, Takami T, Yuasa H, Takei T, Kikuchi K. Two distinct antigen systems in human B lymphocytes: identification of cell surface and intracellular antigens using monoclonal antibodies. Clin Exp Immunol. 1984 Oct;58(1):183–192. [PMC free article] [PubMed]
  • Stashenko P, Nadler LM, Hardy R, Schlossman SF. Characterization of a human B lymphocyte-specific antigen. J Immunol. 1980 Oct;125(4):1678–1685. [PubMed]
  • Lukes RJ, Butler JJ. The pathology and nomenclature of Hodgkin's disease. Cancer Res. 1966 Jun;26(6):1063–1083. [PubMed]
  • Ramsay AD, Smith WJ, Earl HM, Souhami RL, Isaacson PG. T-cell lymphomas in adults: a clinicopathological study of eighteen cases. J Pathol. 1987 Jun;152(2):63–76. [PubMed]
  • Norton AJ, Isaacson PG. The diagnosis of malignant lymphoma using monoclonal antibodies reactive in routinely fixed wax embedded tissue. J Pathol. 1987 Mar;151(3):183–184. [PubMed]
  • Smith SH, Brown MH, Rowe D, Callard RE, Beverley PC. Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1. Immunology. 1986 May;58(1):63–70. [PMC free article] [PubMed]
  • Norton AJ, Ramsay AD, Smith SH, Beverley PC, Isaacson PG. Monoclonal antibody (UCHL1) that recognises normal and neoplastic T cells in routinely fixed tissues. J Clin Pathol. 1986 Apr;39(4):399–405. [PMC free article] [PubMed]
  • Hansmann ML, Wacker HH, Radzun HJ. Paragranuloma is a variant of Hodgkin's disease with predominance of B-cells. Virchows Arch A Pathol Anat Histopathol. 1986;409(2):171–181. [PubMed]
  • Stein H, Mason DY, Gerdes J, O'Connor N, Wainscoat J, Pallesen G, Gatter K, Falini B, Delsol G, Lemke H, et al. The expression of the Hodgkin's disease associated antigen Ki-1 in reactive and neoplastic lymphoid tissue: evidence that Reed-Sternberg cells and histiocytic malignancies are derived from activated lymphoid cells. Blood. 1985 Oct;66(4):848–858. [PubMed]
  • Timens W, Visser L, Poppema S. Nodular lymphocyte predominance type of Hodgkin's disease is a germinal center lymphoma. Lab Invest. 1986 Apr;54(4):457–461. [PubMed]
  • Pinkus GS, Said JW. Hodgkin's disease, lymphocyte predominance type, nodular--a distinct entity? Unique staining profile for L&H variants of Reed-Sternberg cells defined by monoclonal antibodies to leukocyte common antigen, granulocyte-specific antigen, and B-cell-specific antigen. Am J Pathol. 1985 Jan;118(1):1–6. [PMC free article] [PubMed]
  • Stein H, Gerdes J, Lemke H, Mason DY. Evidence of Sternberg-Reed cells being derived from activated lymphocytes. Haematol Blood Transfus. 1985;29:441–444. [PubMed]
  • Day-case surgery. Lancet. 1986 Jan 11;1(8472):78–78. [PubMed]
  • Weiss LM, Strickler JG, Hu E, Warnke RA, Sklar J. Immunoglobulin gene rearrangements in Hodgkin's disease. Hum Pathol. 1986 Oct;17(10):1009–1014. [PubMed]
  • Griesser H, Feller A, Lennert K, Minden M, Mak TW. Rearrangement of the beta chain of the T cell antigen receptor and immunoglobulin genes in lymphoproliferative disorders. J Clin Invest. 1986 Nov;78(5):1179–1184. [PMC free article] [PubMed]

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