pmc logo image
Logo of biochemjBJ Latest papers and much more!
Journal List > Biochem J > v.304(Pt 3); Dec 15, 1994

Formats:
Biochem J. 1994 December 15; 304(Pt 3): 687–692.
PMCID: PMC1137389
Copyright notice
Purification, cDNA cloning and heterologous expression of the human mitochondrial NADP(+)-dependent malic enzyme.
G Loeber, I Maurer-Fogy, and R Schwendenwein
Bender & Co., Vienna, Austria.
Small right arrow pointing to: This article has been cited by other articles in PMC.
Abstract
Mitochondrial NADP(+)-dependent malic enzyme (ME; EC 1.1.1.39) has been purified to homogeneity and characterized kinetically from bovine heart. Partial amino acid sequence information allowed amplification of a specific bovine cDNA, which was used to isolate a full-length human cDNA of this isoform of ME. The cDNA is 1930 bp long and codes for a protein of 604 amino acids. Comparison of the amino acid sequence of this isoform with published sequences of other human ME isoforms shows stretches of homology interrupted by larger regions with significant differences. The human protein has been expressed in Escherichia coli, and the recombinant human protein has the same kinetic properties as the corresponding protein purified from bovine heart. Northern blot analysis showed a strong tissue-specific transcription with a predominantly high expression-rate in organs with a low division-rate.
Full text
Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.2M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.
 
icon of scanned page 687
687
icon of scanned page 688
688
icon of scanned page 689
689
icon of scanned page 690
690
icon of scanned page 691
691
icon of scanned page 692
692
 
Images in this article
Figure 1
Figure 1

Figure 1
on p.689
Figure 4
Figure 4

Figure 4
on p.690
Click on the image to see a larger version.
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
  • Bagchi S, Wise LS, Brown ML, Bregman D, Sul HS, Rubin CS. Structure and expression of murine malic enzyme mRNA. Differentiation-dependent accumulation of two forms of malic enzyme mRNA in 3T3-L1 cells. J Biol Chem. 1987 Feb 5;262(4):1558–1565. [PubMed]
  • Benton WD, Davis RW. Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977 Apr 8;196(4286):180–182. [PubMed]
  • Biegniewska A, Skorkowski EF. Mitochondrial NADP-dependent malic enzyme of cod heart. Rate of forward and reverse reaction. Comp Biochem Physiol B. 1987;86(4):731–735. [PubMed]
  • Chang GG, Wang JK, Huang TM, Lee HJ, Chou WY, Meng CL. Purification and characterization of the cytosolic NADP(+)-dependent malic enzyme from human breast cancer cell line. Eur J Biochem. 1991 Dec 5;202(2):681–688. [PubMed]
  • Church GM, Gilbert W. Genomic sequencing. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991–1995. [PubMed]
  • Dozin B, Magnuson MA, Nikodem VM. Tissue-specific regulation of two functional malic enzyme mRNAs by triiodothyronine. Biochemistry. 1985 Sep 24;24(20):5581–5586. [PubMed]
  • Feinberg AP, Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. [PubMed]
  • Frenkel R. Regulation and physiological functions of malic enzymes. Curr Top Cell Regul. 1975;9:157–181. [PubMed]
  • Kulkarni G, Cook PF, Harris BG. Cloning and nucleotide sequence of a full-length cDNA encoding Ascaris suum malic enzyme. Arch Biochem Biophys. 1993 Jan;300(1):231–237. [PubMed]
  • Lin RC, Davis EJ. Malic enzymes of rabbit heart mitochondria. Separation and comparison of some characteristics of a nicotinamide adenine dinucleotide-preferring and a nicotinamide adenine dinucleotide phosphate-specific enzyme. J Biol Chem. 1974 Jun 25;249(12):3867–3875. [PubMed]
  • Loeber G, Infante AA, Maurer-Fogy I, Krystek E, Dworkin MB. Human NAD(+)-dependent mitochondrial malic enzyme. cDNA cloning, primary structure, and expression in Escherichia coli. J Biol Chem. 1991 Feb 15;266(5):3016–3021. [PubMed]
  • Loeber G, Dworkin MB, Infante A, Ahorn H. Characterization of cytosolic malic enzyme in human tumor cells. FEBS Lett. 1994 May 16;344(2-3):181–186. [PubMed]
  • Magnuson MA, Morioka H, Tecce MF, Nikodem VM. Coding nucleotide sequence of rat liver malic enzyme mRNA. J Biol Chem. 1986 Jan 25;261(3):1183–1186. [PubMed]
  • Mandella RD, Sauer LA. The mitochondrial malic enzymes. I. Submitochondrial localization and purification and properties of the NAD(P)+-dependent enzyme from adrenal cortex. J Biol Chem. 1975 Aug 10;250(15):5877–5884. [PubMed]
  • McKeehan WL. Glycolysis, glutaminolysis and cell proliferation. Cell Biol Int Rep. 1982 Jul;6(7):635–650. [PubMed]
  • Nagel WO, Dauchy RT, Sauer LA. Mitochondrial malic enzymes. An association between NAD(P)+-dependent malic enzyme and cell renewal in Sprague-Dawley rat tissues. J Biol Chem. 1980 May 10;255(9):3849–3854. [PubMed]
  • Petty KJ, Desvergne B, Mitsuhashi T, Nikodem VM. Identification of a thyroid hormone response element in the malic enzyme gene. J Biol Chem. 1990 May 5;265(13):7395–7400. [PubMed]
  • Rothermel BA, Nelson T. Primary structure of the maize NADP-dependent malic enzyme. J Biol Chem. 1989 Nov 25;264(33):19587–19592. [PubMed]
  • Saito T, Tomita K. Malic enzyme activity in heart. J Biochem. 1972 Oct;72(4):807–815. [PubMed]
  • Satterlee J, Hsu RY. Duck liver malic enzyme: sequence of a tryptic peptide containing the cysteine residue labeled by the substrate analog bromopyruvate. Biochim Biophys Acta. 1991 Sep 20;1079(3):247–252. [PubMed]
  • Sauer LA. An NAD- and NADP-dependent malic enzyme with regulatory properties in rat liver and adrenal cortex mitochondrial fractions. Biochem Biophys Res Commun. 1973 Jan 23;50(2):524–531. [PubMed]
  • Sauer LA, Dauchy RT. Identification and properties of the nicotinamide adenine dinucleotide (phosphate)+-dependent malic enzyme in mouse ascites tumor mitochondria. Cancer Res. 1978 Jun;38(6):1751–1756. [PubMed]
  • Simpson ER, Estabrook RW. Mitochondrial malic enzyme: the source of reduced nicotinamide adenine dinucleotide phosphate for steroid hydroxylation in bovine adrenal cortex mitochondria. Arch Biochem Biophys. 1969 Jan;129(1):384–395. [PubMed]
  • Taroni F, Gellera C, Di Donato S. Evidence for two distinct mitochondrial malic enzymes in human skeletal muscle: purification and properties of the NAD(P)+-dependent enzyme. Biochim Biophys Acta. 1987 Dec 18;916(3):446–454. [PubMed]
  • von Heijne G. Mitochondrial targeting sequences may form amphiphilic helices. EMBO J. 1986 Jun;5(6):1335–1342. [PubMed]
  • Yanisch-Perron C, Vieira J, Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. [PubMed]
  • Zelewski M, Swierczyński J. Malic enzyme in human liver. Intracellular distribution, purification and properties of cytosolic isozyme. Eur J Biochem. 1991 Oct 15;201(2):339–345. [PubMed]
  • Zołnierowicz S, Swierczyński J, Zelewski L. Purification and properties of the NAD(P)-dependent malic enzyme from human placental mitochondria. Biochem Med Metab Biol. 1988 Apr;39(2):208–216. [PubMed]
Articles from Biochemical Journal are provided here courtesy of
The Biochemical Society

PubMed articles by these authors