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Biochem J. Jan 15, 1990; 265(2): 321–326.
PMCID: PMC1136890

The epsilon-subunit of ATP synthase from bovine heart mitochondria. Complementary DNA sequence, expression in bovine tissues and evidence of homologous sequences in man and rat.


The epsilon-subunit of ATP synthase from bovine heart mitochondria is assembled into the extrinsic membrane sector, F1-ATPase. The mature protein is 50 amino acid residues in length and its function is unknown. It is a nuclear gene product that is imported into the organelle. A mixture of 64 oligonucleotides 17 bases long, designed on the basis of the known protein sequence, was synthesized and used as a hybridization probe to isolate a cognate cDNA clone from a bovine library. The DNA sequence of this clone was determined, and the protein sequence of the epsilon-subunit deduced from it agrees exactly with that determined by direct sequence analysis of the protein isolated from bovine hearts. The bovine cDNA was used as a hybridization probe to examine the expression of the epsilon-subunit in various bovine tissues. mRNAs related to the cDNA are found in all of these tissues, and no evidence was obtained of the presence of mRNAs for the epsilon-subunit with similar coding sequences and dissimilar 3' non-coding regions. By hybridization experiments with digests of DNA from cow, man and rat it has been shown that sequences related to the bovine cDNA are present in the genomes of all three species. More than one related sequence was detected in all cases, indicating the presence in all three genomes of more than one gene and/or pseudogenes.

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Selected References

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  • Adrian GS, McCammon MT, Montgomery DL, Douglas MG. Sequences required for delivery and localization of the ADP/ATP translocator to the mitochondrial inner membrane. Mol Cell Biol. 1986 Feb;6(2):626–634. [PMC free article] [PubMed]
  • Arends H, Sebald W. Nucleotide sequence of the cloned mRNA and gene of the ADP/ATP carrier from Neurospora crassa. EMBO J. 1984 Feb;3(2):377–382. [PMC free article] [PubMed]
  • Baker A, Leaver CJ. Isolation and sequence analysis of a cDNA encoding the ATP/ADP translocator of Zea mays L. Nucleic Acids Res. 1985 Aug 26;13(16):5857–5867. [PMC free article] [PubMed]
  • Battini R, Ferrari S, Kaczmarek L, Calabretta B, Chen ST, Baserga R. Molecular cloning of a cDNA for a human ADP/ATP carrier which is growth-regulated. J Biol Chem. 1987 Mar 25;262(9):4355–4359. [PubMed]
  • Biggin MD, Gibson TJ, Hong GF. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963–3965. [PMC free article] [PubMed]
  • Breen GA. Bovine liver cDNA clones encoding a precursor of the alpha-subunit of the mitochondrial ATP synthase complex. Biochem Biophys Res Commun. 1988 Apr 15;152(1):264–269. [PubMed]
  • Catterall WA, Pedersen PL. Adenosine triphosphatase from rat liver mitochondria. I. Purification, homogeneity, and physical properties. J Biol Chem. 1971 Aug 25;246(16):4987–4994. [PubMed]
  • Catterall WA, Coty WA, Pedersen PL. Adenosine triphosphatase from rat liver mitochondria. 3. Subunit composition. J Biol Chem. 1973 Nov 10;248(21):7427–7431. [PubMed]
  • Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. [PubMed]
  • Cozens AL, Runswick MJ, Walker JE. DNA sequences of two expressed nuclear genes for human mitochondrial ADP/ATP translocase. J Mol Biol. 1989 Mar 20;206(2):261–280. [PubMed]
  • Dyer MR, Gay NJ, Walker JE. DNA sequences of a bovine gene and of two related pseudogenes for the proteolipid subunit of mitochondrial ATP synthase. Biochem J. 1989 May 15;260(1):249–258. [PMC free article] [PubMed]
  • Gait MJ, Matthes HW, Singh M, Sproat BS, Titmas RC. Rapid synthesis of oligodeoxyribonucleotides. VII. Solid phase synthesis of oligodeoxyribonucleotides by a continuous flow phosphotriester method on a kieselguhr-polyamide support. Nucleic Acids Res. 1982 Oct 25;10(20):6243–6254. [PMC free article] [PubMed]
  • Gay NJ, Walker JE. Molecular cloning of a bovine cathepsin. Biochem J. 1985 Feb 1;225(3):707–712. [PMC free article] [PubMed]
  • Gay NJ, Walker JE. Two genes encoding the bovine mitochondrial ATP synthase proteolipid specify precursors with different import sequences and are expressed in a tissue-specific manner. EMBO J. 1985 Dec 16;4(13A):3519–3524. [PMC free article] [PubMed]
  • Houldsworth J, Attardi G. Two distinct genes for ADP/ATP translocase are expressed at the mRNA level in adult human liver. Proc Natl Acad Sci U S A. 1988 Jan;85(2):377–381. [PMC free article] [PubMed]
  • Messing J, Vieira J. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene. 1982 Oct;19(3):269–276. [PubMed]
  • MITCHELL P. Coupling of phosphorylation to electron and hydrogen transfer by a chemi-osmotic type of mechanism. Nature. 1961 Jul 8;191:144–148. [PubMed]
  • Neckelmann N, Li K, Wade RP, Shuster R, Wallace DC. cDNA sequence of a human skeletal muscle ADP/ATP translocator: lack of a leader peptide, divergence from a fibroblast translocator cDNA, and coevolution with mitochondrial DNA genes. Proc Natl Acad Sci U S A. 1987 Nov;84(21):7580–7584. [PMC free article] [PubMed]
  • Pilkington SJ, Walker JE. Mitochondrial NADH-ubiquinone reductase: complementary DNA sequences of import precursors of the bovine and human 24-kDa subunit. Biochemistry. 1989 Apr 18;28(8):3257–3264. [PubMed]
  • Powell SJ, Medd SM, Runswick MJ, Walker JE. Two bovine genes for mitochondrial ADP/ATP translocase expressed differences in various tissues. Biochemistry. 1989 Jan 24;28(2):866–873. [PubMed]
  • Proudfoot NJ, Brownlee GG. 3' non-coding region sequences in eukaryotic messenger RNA. Nature. 1976 Sep 16;263(5574):211–214. [PubMed]
  • Runswick MJ, Powell SJ, Nyren P, Walker JE. Sequence of the bovine mitochondrial phosphate carrier protein: structural relationship to ADP/ATP translocase and the brown fat mitochondria uncoupling protein. EMBO J. 1987 May;6(5):1367–1373. [PMC free article] [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]
  • Staden R. Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing. Nucleic Acids Res. 1982 Aug 11;10(15):4731–4751. [PMC free article] [PubMed]
  • Staden R. Computer methods to locate signals in nucleic acid sequences. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):505–519. [PMC free article] [PubMed]
  • Tzagoloff A. Assembly of the mitochondrial membrane system. I. Characterization of some enzymes of the inner membrane of yeast mitochondria. J Biol Chem. 1969 Sep 25;244(18):5020–5026. [PubMed]
  • Velours J, Arselin de Chateaubodeau G, Galante M, Guérin B. Subunit 4 of ATP synthase (F0F1) from yeast mitochondria. Purification, amino-acid composition and partial N-terminal sequence. Eur J Biochem. 1987 May 4;164(3):579–584. [PubMed]
  • Walker JE, Runswick MJ, Saraste M. Subunit equivalence in Escherichia coli and bovine heart mitochondrial F1F0 ATPases. FEBS Lett. 1982 Sep 20;146(2):393–396. [PubMed]
  • Walker JE, Fearnley IM, Gay NJ, Gibson BW, Northrop FD, Powell SJ, Runswick MJ, Saraste M, Tybulewicz VL. Primary structure and subunit stoichiometry of F1-ATPase from bovine mitochondria. J Mol Biol. 1985 Aug 20;184(4):677–701. [PubMed]
  • Walker JE, Gay NJ, Powell SJ, Kostina M, Dyer MR. ATP synthase from bovine mitochondria: sequences of imported precursors of oligomycin sensitivity conferral protein, factor 6, and adenosinetriphosphatase inhibitor protein. Biochemistry. 1987 Dec 29;26(26):8613–8619. [PubMed]
  • Walker JE, Runswick MJ, Poulter L. ATP synthase from bovine mitochondria. The characterization and sequence analysis of two membrane-associated sub-units and of the corresponding cDNAs. J Mol Biol. 1987 Sep 5;197(1):89–100. [PubMed]
  • Walker JE, Powell SJ, Viñas O, Runswick MJ. ATP synthase from bovine mitochondria: complementary DNA sequence of the import precursor of a heart isoform of the alpha subunit. Biochemistry. 1989 May 30;28(11):4702–4708. [PubMed]

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