• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of biochemjBJ Latest papers and much more!
Biochem J. Jan 1, 1995; 305(Pt 1): 81–85.
PMCID: PMC1136432

Cloning and functional expression of a Na(+)-dependent phosphate co-transporter from human kidney: cDNA cloning and functional expression.

Abstract

A cDNA clone encoding a protein 69% identical in amino acid sequence with that of the Na/P(i) co-transporter NaP(i)-1 was isolated from a human kidney cDNA library. The DNA sequence was identical with that of NPT-1 cDNA published by Chong, Kristjansson, Zoghbi and Hughe (1993) (Genomics, 18, 355-359). In the present study, we have characterized the function of the encoded protein and the tissue distribution of its mRNA. Injection of RNA transcribed from NPT-1 into Xenopus oocytes resulted in expression of Na/P(i) co-transport activity showing a high affinity for P(i) transport (Km 0.29 mM). Kinetic characterization ([P(i)], [Na+]) demonstrated that the expressed transport activity has properties similar to those displayed by oocytes injected with human kidney poly(A)+ RNA. Northern blotting demonstrated that NPT-1 mRNA is expressed in renal cortex, liver and brain but not in other tissues. Hybrid depletion with antisense oligonucleotides to NaP(i)-3 and NPT-1 completely inhibited poly(A)+ RNA-induced Na(+)-dependent P(i) uptake in oocytes. These findings indicate that two high-affinity Na/P(i) cotransporters (NaP(i)-3 and NPT-1) are present in human kidney cortex.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (864K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Werner A, Moore ML, Mantei N, Biber J, Semenza G, Murer H. Cloning and expression of cDNA for a Na/Pi cotransport system of kidney cortex. Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9608–9612. [PMC free article] [PubMed]
  • Magagnin S, Werner A, Markovich D, Sorribas V, Stange G, Biber J, Murer H. Expression cloning of human and rat renal cortex Na/Pi cotransport. Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):5979–5983. [PMC free article] [PubMed]
  • Sorribas V, Markovich D, Hayes G, Stange G, Forgo J, Biber J, Murer H. Cloning of a Na/Pi cotransporter from opossum kidney cells. J Biol Chem. 1994 Mar 4;269(9):6615–6621. [PubMed]
  • Brunette MG, Chan M, Maag U, Béliveau R. Phosphate uptake by superficial and deep nephron brush border membranes. Effect of the dietary phosphate and parathyroid hormone. Pflugers Arch. 1984 Apr;400(4):356–362. [PubMed]
  • Bindels RJ, van den Broek LA, van Os CH. Effect of pH on the kinetics of Na+-dependent phosphate transport in rat renal brush-border membranes. Biochim Biophys Acta. 1987 Feb 12;897(1):83–92. [PubMed]
  • Walker JJ, Yan TS, Quamme GA. Presence of multiple sodium-dependent phosphate transport processes in proximal brush-border membrane. Am J Physiol. 1987 Feb;252(2 Pt 2):F226–F231. [PubMed]
  • Tenenhouse HS, Klugerman AH, Neal JL. Effect of phosphonoformic acid, dietary phosphate and the Hyp mutation on kinetically distinct phosphate transport processes in mouse kidney. Biochim Biophys Acta. 1989 Sep 4;984(2):207–213. [PubMed]
  • Chong SS, Kristjansson K, Zoghbi HY, Hughes MR. Molecular cloning of the cDNA encoding a human renal sodium phosphate transport protein and its assignment to chromosome 6p21.3-p23. Genomics. 1993 Nov;18(2):355–359. [PubMed]
  • Miyamoto K, Hase K, Taketani Y, Minami H, Oka T, Nakabou Y, Hagihira H. Diabetes and glucose transporter gene expression in rat small intestine. Biochem Biophys Res Commun. 1991 Dec 31;181(3):1110–1117. [PubMed]
  • Miyamoto K, Hase K, Taketani Y, Minami H, Oka T, Nakabou Y, Hagihira H. Developmental changes in intestinal glucose transporter mRNA levels. Biochem Biophys Res Commun. 1992 Mar 16;183(2):626–631. [PubMed]
  • Contreras R, Cheroutre H, Degrave W, Fiers W. Simple, efficient in vitro synthesis of capped RNA useful for direct expression of cloned eukaryotic genes. Nucleic Acids Res. 1982 Oct 25;10(20):6353–6362. [PMC free article] [PubMed]
  • Surin BP, Jans DA, Fimmel AL, Shaw DC, Cox GB, Rosenberg H. Structural gene for the phosphate-repressible phosphate-binding protein of Escherichia coli has its own promoter: complete nucleotide sequence of the phoS gene. J Bacteriol. 1984 Mar;157(3):772–778. [PMC free article] [PubMed]
  • Ghishan FK, Rebeiz R, Honda T, Nakagawa N. Characterization and expression of a novel Na(+)-inorganic phosphate transporter at the liver plasma membrane of the rat. Gastroenterology. 1993 Aug;105(2):519–526. [PubMed]
  • Wehrle JP, Pedersen PL. Characteristics of phosphate uptake by Ehrlich ascites tumor cells. J Biol Chem. 1982 Aug 25;257(16):9698–9703. [PubMed]
  • Biber J, Custer M, Werner A, Kaissling B, Murer H. Localization of NaPi-1, a Na/Pi cotransporter, in rabbit kidney proximal tubules. II. Localization by immunohistochemistry. Pflugers Arch. 1993 Aug;424(3-4):210–215. [PubMed]
  • Custer M, Meier F, Schlatter E, Greger R, Garcia-Perez A, Biber J, Murer H. Localization of NaPi-1, a Na-Pi cotransporter, in rabbit kidney proximal tubules. I. mRNA localization by reverse transcription/polymerase chain reaction. Pflugers Arch. 1993 Aug;424(3-4):203–209. [PubMed]
  • Delisle MC, Boyer C, Vachon V, Giroux S, Béliveau R. Immunodetection and characterization of proteins implicated in renal sodium/phosphate cotransport. Biochim Biophys Acta. 1994 Mar 23;1190(2):289–296. [PubMed]
  • Brunette MG, Chan M, Maag U, Béliveau R. Phosphate uptake by superficial and deep nephron brush border membranes. Effect of the dietary phosphate and parathyroid hormone. Pflugers Arch. 1984 Apr;400(4):356–362. [PubMed]
  • Werner A, Kempson SA, Biber J, Murer H. Increase of Na/Pi-cotransport encoding mRNA in response to low Pi diet in rat kidney cortex. J Biol Chem. 1994 Mar 4;269(9):6637–6639. [PubMed]
  • Tenenhouse HS, Henry HL. Protein kinase activity and protein kinase inhibitor in mouse kidney: effect of the X-linked Hyp mutation and vitamin D status. Endocrinology. 1985 Nov;117(5):1719–1726. [PubMed]
  • Scriver CR, Tenenhouse HS. X-linked hypophosphataemia: a homologous phenotype in humans and mice with unusual organ-specific gene dosage. J Inherit Metab Dis. 1992;15(4):610–624. [PubMed]
  • Tenenhouse HS, Werner A, Biber J, Ma S, Martel J, Roy S, Murer H. Renal Na(+)-phosphate cotransport in murine X-linked hypophosphatemic rickets. Molecular characterization. J Clin Invest. 1994 Feb;93(2):671–676. [PMC free article] [PubMed]
  • Kos CH, Tihy F, Econs MJ, Murer H, Lemieux N, Tenenhouse HS. Localization of a renal sodium-phosphate cotransporter gene to human chromosome 5q35. Genomics. 1994 Jan 1;19(1):176–177. [PubMed]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...