|
|
Biochem J. 1990 February 1; 265(3): 659–665. | PMCID: PMC1133685 |
Modulation of fibroblast proliferation by oxygen free radicals. G A Murrell, M J Francis, and L Bromley Nuffield Department of Orthopaedic Surgery, University of Oxford, U.K. Abstract The major unexplained phenomenon in fibrotic conditions is an increase in replicating fibroblasts. In this report we present evidence that oxygen free radicals can both stimulate and inhibit proliferation of cultured human fibroblasts, and that fibroblasts themselves release superoxide (O2.-) free radicals. Fibroblasts released O2.- in concentrations which stimulated proliferation, a finding confirmed by a dose-dependent inhibition of proliferation by free radical scavengers. Oxygen free radicals released by a host of agents may thus provide a very fast, specific and sensitive trigger for fibroblast proliferation. Prolonged stimulation may result in fibrosis, and agents which inhibit free radical release may have a role in the prevention of fibrosis. Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.2M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References. Images in this article Click on the image to see a larger version. These references are in PubMed. This may not be the complete list of references from this article. - Ager A, Gordon JL. Differential effects of hydrogen peroxide on indices of endothelial cell function. J Exp Med. 1984 Feb 1;159(2):592–603. [PubMed]
- Ager A, Wenham DJ, Gordon JL. Stimulation of endothelial cells by protease activity in commercial preparations of xanthine oxidase. Thromb Res. 1984 Jul 1;35(1):43–52. [PubMed]
- Brickley-Parsons D, Glimcher MJ, Smith RJ, Albin R, Adams JP. Biochemical changes in the collagen of the palmar fascia in patients with Dupuytren's disease. J Bone Joint Surg Am. 1981 Jun;63(5):787–797. [PubMed]
- Bruder G, Heid HW, Jarasch ED, Mather IH. Immunological identification and determination of xanthine oxidase in cells and tissues. Differentiation. 1983;23(3):218–225. [PubMed]
- Burkhardt H, Schwingel M, Menninger H, Macartney HW, Tschesche H. Oxygen radicals as effectors of cartilage destruction. Direct degradative effect on matrix components and indirect action via activation of latent collagenase from polymorphonuclear leukocytes. Arthritis Rheum. 1986 Mar;29(3):379–387. [PubMed]
- Gabbiani G, Majno G. Dupuytren's contracture: fibroblast contraction? An ultrastructural study. Am J Pathol. 1972 Jan;66(1):131–146. [PubMed]
- Greenwald RA, Moy WW. Effect of oxygen-derived free radicals on hyaluronic acid. Arthritis Rheum. 1980 Apr;23(4):455–463. [PubMed]
- Jones CE, Crowell JW, Smith EE. Significance of increased blood uric acid following extensive hemorrhage. Am J Physiol. 1968 Jun;214(6):1374–1377. [PubMed]
- MACCALLUM P, HUESTON JT. The pathology of Dupuytren's contracture. Aust N Z J Surg. 1962 May;31:241–253. [PubMed]
- Matsubara T, Ziff M. Superoxide anion release by human endothelial cells: synergism between a phorbol ester and a calcium ionophore. J Cell Physiol. 1986 May;127(2):207–210. [PubMed]
- McCord JM. Oxygen-derived free radicals in postischemic tissue injury. N Engl J Med. 1985 Jan 17;312(3):159–163. [PubMed]
- McKelvey TG, Höllwarth ME, Granger DN, Engerson TD, Landler U, Jones HP. Mechanisms of conversion of xanthine dehydrogenase to xanthine oxidase in ischemic rat liver and kidney. Am J Physiol. 1988 May;254(5 Pt 1):G753–G760. [PubMed]
- Meikle MC, Heath JK, Hembry RM, Reynolds JJ. Rabbit cranial suture fibroblasts under tension express a different collagen phenotype. Arch Oral Biol. 1982;27(7):609–613. [PubMed]
- Mello Filho AC, Hoffmann ME, Meneghini R. Cell killing and DNA damage by hydrogen peroxide are mediated by intracellular iron. Biochem J. 1984 Feb 15;218(1):273–275. [PubMed]
- Moorhouse PC, Grootveld M, Halliwell B, Quinlan JG, Gutteridge JM. Allopurinol and oxypurinol are hydroxyl radical scavengers. FEBS Lett. 1987 Mar 9;213(1):23–28. [PubMed]
- Murrell GA, Francis MJ, Bromley L. Free radicals and Dupuytren's contracture. Br Med J (Clin Res Ed). 1987 Nov 28;295(6610):1373–1375. [PubMed]
- Oreffo RO, Francis JA, Triffitt JT. Vitamin A effects on UMR 106 osteosarcoma cells are not mediated by specific cytosolic receptors. Biochem J. 1985 Dec 1;232(2):599–603. [PubMed]
- Parks DA, Williams TK, Beckman JS. Conversion of xanthine dehydrogenase to oxidase in ischemic rat intestine: a reevaluation. Am J Physiol. 1988 May;254(5 Pt 1):G768–G774. [PubMed]
- Phan SH, Fantone JC. Inhibition of bleomycin-induced pulmonary fibrosis by lipopolysaccharide. Lab Invest. 1984 May;50(5):587–591. [PubMed]
- Puzas JE, Brand JS. The effect of bone cell stimulatory factors can be measured with thymidine incorporation only under specific conditions. Calcif Tissue Int. 1986 Aug;39(2):104–108. [PubMed]
- Ratych RE, Chuknyiska RS, Bulkley GB. The primary localization of free radical generation after anoxia/reoxygenation in isolated endothelial cells. Surgery. 1987 Aug;102(2):122–131. [PubMed]
- Thornalley P, Wolff S, Crabbe J, Stern A. The autoxidation of glyceraldehyde and other simple monosaccharides under physiological conditions catalysed by buffer ions. Biochim Biophys Acta. 1984 Feb 14;797(2):276–287. [PubMed]
- Tritsch GL, Niswander PW. Purine catabolism as a source of superoxide in macrophages. Ann N Y Acad Sci. 1985;451:279–290. [PubMed]
- Wach F, Hein R, Adelmann-Grill BC, Krieg T. Inhibition of fibroblast chemotaxis by superoxide dismutase. Eur J Cell Biol. 1987 Aug;44(1):124–127. [PubMed]
- Weening RS, Wever R, Roos D. Quantitative aspects of the production of superoxide radicals by phagocytizing human granulocytes. J Lab Clin Med. 1975 Feb;85(2):245–252. [PubMed]
|
This article has been 