• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of biochemjBJ Latest papers and much more!
Biochem J. Dec 1, 1992; 288(Pt 2): 545–551.
PMCID: PMC1132045

Erythroid expression and DNAaseI-hypersensitive sites of the carbonic anhydrase 1 gene.

Abstract

The carbonic anhydrase 1 gene is expressed in adult human and mouse erythroid cells and colon epithelia from two distinct promoters. We have explored the erythroid promoter for cis-acting sequences involved in transcription using DNAaseI as a probe. Two DNAaseI-hypersensitive sites (DHS-1 and DHS-2) have been identified in the distal erythroid promoter in CA1-expressing erythroleukaemic cells. These sites are present at low levels in K562 cells, which have a foetal/embryonic phenotype and do not express CA1. DHS-1 and DHS-2 are not present in non-erythroid cells, including colon cells, which express CA1 from the proximal colon promoter. DHS-1 and DHS-2 were also generated in an heterologous CA1 gene containing 5 kb of erythroid promoter sequence after transfection into erythroid cells, including K562 cells. These transfection studies showed that both this fragment, and an abbreviated 817 bp promoter fragment which contains only DHS-1, were sufficient to confer erythroid-specific expression to a reporter gene. These promoters were active in cell lines expressing CA1 and in K562 cells. This latter observation implies that a developmental repressor factor is both present in K562 cells and binds to a cis-acting sequence that is absent from the sequence 5 kb upstream of the erythroid transcription start site.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (2.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Abbott C. Characterization of mouse-hamster somatic cell hybrids by PCR: a panel of mouse-specific primers for each chromosome. Mamm Genome. 1992;2(2):106–109. [PubMed]
  • Antoniou M, deBoer E, Habets G, Grosveld F. The human beta-globin gene contains multiple regulatory regions: identification of one promoter and two downstream enhancers. EMBO J. 1988 Feb;7(2):377–384. [PMC free article] [PubMed]
  • Beechey C, Tweedie S, Spurr N, Ball S, Peters J, Edwards Y. Mapping of mouse carbonic anhydrase-3, Car-3: another locus in the homologous region of mouse chromosome 3 and human chromosome 8. Genomics. 1990 Apr;6(4):692–696. [PubMed]
  • Blom van Assendelft G, Hanscombe O, Grosveld F, Greaves DR. The beta-globin dominant control region activates homologous and heterologous promoters in a tissue-specific manner. Cell. 1989 Mar 24;56(6):969–977. [PubMed]
  • Brady HJ, Sowden JC, Edwards M, Lowe N, Butterworth PH. Multiple GF-1 binding sites flank the erythroid specific transcription unit of the human carbonic anhydrase I gene. FEBS Lett. 1989 Nov 6;257(2):451–456. [PubMed]
  • Brady HJ, Edwards M, Linch DC, Knott L, Barlow JH, Butterworth PH. Expression of the human carbonic anhydrase I gene is activated late in fetal erythroid development and regulated by stage-specific trans-acting factors. Br J Haematol. 1990 Sep;76(1):135–142. [PubMed]
  • Brady HJ, Lowe N, Sowden JC, Edwards M, Butterworth PH. The human carbonic anhydrase I gene has two promoters with different tissue specificities. Biochem J. 1991 Aug 1;277(Pt 3):903–905. [PMC free article] [PubMed]
  • Brownson C, Isenberg H, Brown W, Salmons S, Edwards Y. Changes in skeletal muscle gene transcription induced by chronic stimulation. Muscle Nerve. 1988 Nov;11(11):1183–1189. [PubMed]
  • Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. [PubMed]
  • Cordingley MG, Riegel AT, Hager GL. Steroid-dependent interaction of transcription factors with the inducible promoter of mouse mammary tumor virus in vivo. Cell. 1987 Jan 30;48(2):261–270. [PubMed]
  • Donovan-Peluso M, Acuto S, Swanson M, Dobkin C, Bank A. Expression of human gamma-globin genes in human erythroleukemia (K562) cells. J Biol Chem. 1987 Dec 15;262(35):17051–17057. [PubMed]
  • Edwards YH, Sakoda S, Schon E, Povey S. The gene for human muscle-specific phosphoglycerate mutase, PGAM2, mapped to chromosome 7 by polymerase chain reaction. Genomics. 1989 Nov;5(4):948–951. [PubMed]
  • Elgin SC. DNAase I-hypersensitive sites of chromatin. Cell. 1981 Dec;27(3 Pt 2):413–415. [PubMed]
  • Enver T, Brewer AC, Patient RK. Simian virus 40-mediated cis induction of the Xenopus beta-globin DNase I hypersensitive site. Nature. 1985 Dec 19;318(6047):680–683. [PubMed]
  • Enver T, Zhang JW, Anagnou NP, Stamatoyannopoulos G, Papayannopoulou T. Developmental programs of human erythroleukemia cells: globin gene expression and methylation. Mol Cell Biol. 1988 Nov;8(11):4917–4926. [PMC free article] [PubMed]
  • FOLEY GE, LAZARUS H, FARBER S, UZMAN BG, BOONE BA, MCCARTHY RE. CONTINUOUS CULTURE OF HUMAN LYMPHOBLASTS FROM PERIPHERAL BLOOD OF A CHILD WITH ACUTE LEUKEMIA. Cancer. 1965 Apr;18:522–529. [PubMed]
  • Fraser PJ, Curtis PJ. Specific pattern of gene expression during induction of mouse erythroleukemia cells. Genes Dev. 1987 Oct;1(8):855–861. [PubMed]
  • Fraser P, Cummings P, Curtis P. The mouse carbonic anhydrase I gene contains two tissue-specific promoters. Mol Cell Biol. 1989 Aug;9(8):3308–3313. [PMC free article] [PubMed]
  • Friend C, Scher W, Holland JG, Sato T. Hemoglobin synthesis in murine virus-induced leukemic cells in vitro: stimulation of erythroid differentiation by dimethyl sulfoxide. Proc Natl Acad Sci U S A. 1971 Feb;68(2):378–382. [PMC free article] [PubMed]
  • Gross DS, Garrard WT. Nuclease hypersensitive sites in chromatin. Annu Rev Biochem. 1988;57:159–197. [PubMed]
  • Grosveld FG, Lund T, Murray EJ, Mellor AL, Dahl HH, Flavell RA. The construction of cosmid libraries which can be used to transform eukaryotic cells. Nucleic Acids Res. 1982 Nov 11;10(21):6715–6732. [PMC free article] [PubMed]
  • Grosveld F, van Assendelft GB, Greaves DR, Kollias G. Position-independent, high-level expression of the human beta-globin gene in transgenic mice. Cell. 1987 Dec 24;51(6):975–985. [PubMed]
  • Hammer RE, Swift GH, Ornitz DM, Quaife CJ, Palmiter RD, Brinster RL, MacDonald RJ. The rat elastase I regulatory element is an enhancer that directs correct cell specificity and developmental onset of expression in transgenic mice. Mol Cell Biol. 1987 Aug;7(8):2956–2967. [PMC free article] [PubMed]
  • Jewell DA, Tu CK, Paranawithana SR, Tanhauser SM, LoGrasso PV, Laipis PJ, Silverman DN. Enhancement of the catalytic properties of human carbonic anhydrase III by site-directed mutagenesis. Biochemistry. 1991 Feb 12;30(6):1484–1490. [PubMed]
  • Kabat D, Sherton CC, Evans LH, Bigley R, Koler RD. Synthesis of erythrocyte-specific proteins in cultured friend leukemia cells. Cell. 1975 Jul;5(3):331–338. [PubMed]
  • Lönnerholm G. Histochemical localization of carbonic anhydrase in mammalian tissue. Ann N Y Acad Sci. 1984;429:369–381. [PubMed]
  • Lowe N, Brady HJ, Barlow JH, Sowden JC, Edwards M, Butterworth PH. Structure and methylation patterns of the gene encoding human carbonic anhydrase I. Gene. 1990 Sep 14;93(2):277–283. [PubMed]
  • Lowe N, Edwards YH, Edwards M, Butterworth PH. Physical mapping of the human carbonic anhydrase gene cluster on chromosome 8. Genomics. 1991 Aug;10(4):882–888. [PubMed]
  • Lozzio CB, Lozzio BB. Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome. Blood. 1975 Mar;45(3):321–334. [PubMed]
  • Lyons GE, Buckingham ME, Tweedie S, Edwards YH. Carbonic anhydrase III, an early mesodermal marker, is expressed in embryonic mouse skeletal muscle and notochord. Development. 1991 Jan;111(1):233–244. [PubMed]
  • Martin P, Papayannopoulou T. HEL cells: a new human erythroleukemia cell line with spontaneous and induced globin expression. Science. 1982 Jun 11;216(4551):1233–1235. [PubMed]
  • Ney PA, Sorrentino BP, McDonagh KT, Nienhuis AW. Tandem AP-1-binding sites within the human beta-globin dominant control region function as an inducible enhancer in erythroid cells. Genes Dev. 1990 Jun;4(6):993–1006. [PubMed]
  • Pevny L, Simon MC, Robertson E, Klein WH, Tsai SF, D'Agati V, Orkin SH, Costantini F. Erythroid differentiation in chimaeric mice blocked by a targeted mutation in the gene for transcription factor GATA-1. Nature. 1991 Jan 17;349(6306):257–260. [PubMed]
  • Piña B, Brüggemeier U, Beato M. Nucleosome positioning modulates accessibility of regulatory proteins to the mouse mammary tumor virus promoter. Cell. 1990 Mar 9;60(5):719–731. [PubMed]
  • Radice G, Costantini F. Tissue-specific DNase I hypersensitive sites in a foreign globin gene in transgenic mice. Nucleic Acids Res. 1986 Dec 22;14(24):9765–9780. [PMC free article] [PubMed]
  • Shapiro LH, Venta PJ, Tashian RE. Molecular analysis of G+C-rich upstream sequences regulating transcription of the human carbonic anhydrase II gene. Mol Cell Biol. 1987 Dec;7(12):4589–4593. [PMC free article] [PubMed]
  • Smithies O, Gregg RG, Boggs SS, Koralewski MA, Kucherlapati RS. Insertion of DNA sequences into the human chromosomal beta-globin locus by homologous recombination. Nature. 1985 Sep 19;317(6034):230–234. [PubMed]
  • Demmer LA, Birkenmeier EH, Sweetser DA, Levin MS, Zollman S, Sparkes RS, Mohandas T, Lusis AJ, Gordon JI. The cellular retinol binding protein II gene. Sequence analysis of the rat gene, chromosomal localization in mice and humans, and documentation of its close linkage to the cellular retinol binding protein gene. J Biol Chem. 1987 Feb 25;262(6):2458–2467. [PubMed]
  • Tashian RE. The carbonic anhydrases: widening perspectives on their evolution, expression and function. Bioessays. 1989 Jun;10(6):186–192. [PubMed]
  • Tsai SF, Martin DI, Zon LI, D'Andrea AD, Wong GG, Orkin SH. Cloning of cDNA for the major DNA-binding protein of the erythroid lineage through expression in mammalian cells. Nature. 1989 Jun 8;339(6224):446–451. [PubMed]
  • Tu CK, Silverman DN, Forsman C, Jonsson BH, Lindskog S. Role of histidine 64 in the catalytic mechanism of human carbonic anhydrase II studied with a site-specific mutant. Biochemistry. 1989 Sep 19;28(19):7913–7918. [PubMed]
  • Villeval JL, Testa U, Vinci G, Tonthat H, Bettaieb A, Titeux M, Cramer P, Edelman L, Rochant H, Breton-Gorius J, et al. Carbonic anhydrase I is an early specific marker of normal human erythroid differentiation. Blood. 1985 Nov;66(5):1162–1170. [PubMed]
  • Whitehead RH, Macrae FA, St John DJ, Ma J. A colon cancer cell line (LIM1215) derived from a patient with inherited nonpolyposis colorectal cancer. J Natl Cancer Inst. 1985 Apr;74(4):759–765. [PubMed]
  • Wright S, Rosenthal A, Flavell R, Grosveld F. DNA sequences required for regulated expression of beta-globin genes in murine erythroleukemia cells. Cell. 1984 Aug;38(1):265–273. [PubMed]
  • Zon LI, Tsai SF, Burgess S, Matsudaira P, Bruns GA, Orkin SH. The major human erythroid DNA-binding protein (GF-1): primary sequence and localization of the gene to the X chromosome. Proc Natl Acad Sci U S A. 1990 Jan;87(2):668–672. [PMC free article] [PubMed]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...