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Biochem J. Dec 15, 1992; 288(Pt 3): 721–726.
PMCID: PMC1131945

Modulation of aggrecan and link-protein synthesis in articular cartilage.

Abstract

The addition of serum or insulin-like growth factor-I (IGF-I) to the medium of explant cultures of bovine articular cartilage is known to stimulate the synthesis of aggrecan in a dose-dependent manner. The half-life of the pool of proteoglycan core protein was measured in adult articular cartilage cultured for 6 days in the presence and absence of 20 ng of IGF-I/ml and shown to be 24 min under both sets of conditions. The half-life of the mRNA pool coding for aggrecan was also determined and shown to be approx. 4 h in cartilage maintained in culture with or without IGF-I. The pool size of mRNA coding for aggrecan core protein increased 5-6-fold in cartilage explants maintained in culture in medium containing 20% (v/v) fetal-calf serum; however, in tissue maintained with medium containing IGF-I there was no increase in the cellular levels of this mRNA. This suggests that aggrecan synthesis is stimulated by IGF-I at the level of translation of mRNA coding for the core protein of this proteoglycan and that other growth factors are present in serum that stimulate aggrecan synthesis at the level of transcription of the core-protein gene. Inclusion of serum or IGF-I in the medium of cartilage explant cultures induced increases in the amounts of mRNA coding for type II collagen and link protein, whereas only serum enhanced the amount of mRNA for the core protein of decorin.

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Selected References

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  • Curtis AJ, Ng CK, Handley CJ, Robinson HC. Effect of insulin-like growth factor-I on the synthesis and distribution of link protein and hyaluronan in explant cultures of articular cartilage. Biochim Biophys Acta. 1992 Jun 29;1135(3):309–317. [PubMed]
  • Doege K, Hassell JR, Caterson B, Yamada Y. Link protein cDNA sequence reveals a tandemly repeated protein structure. Proc Natl Acad Sci U S A. 1986 Jun;83(11):3761–3765. [PMC free article] [PubMed]
  • Doege K, Sasaki M, Horigan E, Hassell JR, Yamada Y. Complete primary structure of the rat cartilage proteoglycan core protein deduced from cDNA clones. J Biol Chem. 1987 Dec 25;262(36):17757–17767. [PubMed]
  • Fisher LW, Termine JD, Young MF. Deduced protein sequence of bone small proteoglycan I (biglycan) shows homology with proteoglycan II (decorin) and several nonconnective tissue proteins in a variety of species. J Biol Chem. 1989 Mar 15;264(8):4571–4576. [PubMed]
  • Fort P, Marty L, Piechaczyk M, el Sabrouty S, Dani C, Jeanteur P, Blanchard JM. Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family. Nucleic Acids Res. 1985 Mar 11;13(5):1431–1442. [PMC free article] [PubMed]
  • Glisin V, Crkvenjakov R, Byus C. Ribonucleic acid isolated by cesium chloride centrifugation. Biochemistry. 1974 Jun 4;13(12):2633–2637. [PubMed]
  • Handley CJ, Lowther DA. Extracellular matrix metabolism by chondrocytes. III. Modulation of proteoglycan synthesis by extracellular levels of proteoglycan in cartilage cells in culture. Biochim Biophys Acta. 1977 Nov 7;500(1):132–139. [PubMed]
  • Hardingham TE. The role of link-protein in the structure of cartilage proteoglycan aggregates. Biochem J. 1979 Jan 1;177(1):237–247. [PMC free article] [PubMed]
  • Hascall VC, Handley CJ, McQuillan DJ, Hascall GK, Robinson HC, Lowther DA. The effect of serum on biosynthesis of proteoglycans by bovine articular cartilage in culture. Arch Biochem Biophys. 1983 Jul 1;224(1):206–223. [PubMed]
  • Kimura JH, Hardingham TE, Hascall VC. Assembly of newly synthesized proteoglycan and link protein into aggregates in cultures of chondrosarcoma chondrocytes. J Biol Chem. 1980 Aug 10;255(15):7134–7143. [PubMed]
  • Kimura JH, Caputo CB, Hascall VC. The effect of cycloheximide on synthesis of proteoglycans by cultured chondrocytes from the Swarm rat chondrosarcoma. J Biol Chem. 1981 May 10;256(9):4368–4376. [PubMed]
  • Leboy PS, Shapiro IM, Uschmann BD, Oshima O, Lin D. Gene expression in mineralizing chick epiphyseal cartilage. J Biol Chem. 1988 Jun 15;263(17):8515–8520. [PubMed]
  • Levenson RM, Nairn AC, Blackshear PJ. Insulin rapidly induces the biosynthesis of elongation factor 2. J Biol Chem. 1989 Jul 15;264(20):11904–11911. [PubMed]
  • Luyten FP, Hascall VC, Nissley SP, Morales TI, Reddi AH. Insulin-like growth factors maintain steady-state metabolism of proteoglycans in bovine articular cartilage explants. Arch Biochem Biophys. 1988 Dec;267(2):416–425. [PubMed]
  • Manzella JM, Rychlik W, Rhoads RE, Hershey JW, Blackshear PJ. Insulin induction of ornithine decarboxylase. Importance of mRNA secondary structure and phosphorylation of eucaryotic initiation factors eIF-4B and eIF-4E. J Biol Chem. 1991 Feb 5;266(4):2383–2389. [PubMed]
  • McQuillan DJ, Handley CJ, Robinson HC, Ng K, Tzaicos C, Brooks PR, Lowther DA. The relation of protein synthesis to chondroitin sulphate biosynthesis in cultured bovine cartilage. Biochem J. 1984 Dec 15;224(3):977–988. [PMC free article] [PubMed]
  • McQuillan DJ, Handley CJ, Robinson HC, Ng K, Tzaicos C. The relation of RNA synthesis to chondroitin sulphate biosynthesis in cultured bovine cartilage. Biochem J. 1986 Apr 15;235(2):499–505. [PMC free article] [PubMed]
  • McQuillan DJ, Handley CJ, Robinson HC. Control of proteoglycan biosynthesis. Further studies on the effect of serum on cultured bovine articular cartilage. Biochem J. 1986 Aug 1;237(3):741–747. [PMC free article] [PubMed]
  • McQuillan DJ, Handley CJ, Campbell MA, Bolis S, Milway VE, Herington AC. Stimulation of proteoglycan biosynthesis by serum and insulin-like growth factor-I in cultured bovine articular cartilage. Biochem J. 1986 Dec 1;240(2):423–430. [PMC free article] [PubMed]
  • Oegema TR, Jr, Kraft EL, Jourdian GW, Van Valen TR. Phosphorylation of chondroitin sulfate in proteoglycans from the swarm rat chondrosarcoma. J Biol Chem. 1984 Feb 10;259(3):1720–1726. [PubMed]
  • Piechaczyk M, Blanchard JM, Marty L, Dani C, Panabieres F, El Sabouty S, Fort P, Jeanteur P. Post-transcriptional regulation of glyceraldehyde-3-phosphate-dehydrogenase gene expression in rat tissues. Nucleic Acids Res. 1984 Sep 25;12(18):6951–6963. [PMC free article] [PubMed]
  • Schalkwijk J, Joosten LA, van den Berg WB, van Wyk JJ, van de Putte LB. Insulin-like growth factor stimulation of chondrocyte proteoglycan synthesis by human synovial fluid. Arthritis Rheum. 1989 Jan;32(1):66–71. [PubMed]
  • Su MW, Lee B, Ramirez F, Machado M, Horton W. Nucleotide sequence of the full length cDNA encoding for human type II procollagen. Nucleic Acids Res. 1989 Nov 25;17(22):9473–9473. [PMC free article] [PubMed]
  • Tesch GH, Handley CJ, Cornell HJ, Herington AC. Effects of free and bound insulin-like growth factors on proteoglycan metabolism in articular cartilage explants. J Orthop Res. 1992 Jan;10(1):14–22. [PubMed]
  • Thomas G, Martin-Pérez J, Siegmann M, Otto AM. The effect of serum, EGF, PGF2 alpha and insulin on S6 phosphorylation and the initiation of protein and DNA synthesis. Cell. 1982 Aug;30(1):235–242. [PubMed]
  • Towle CA, Mankin HJ, Avruch J, Treadwell BV. Insulin promoted decrease in the phosphorylation of protein synthesis initiation factor eIF-2. Biochem Biophys Res Commun. 1984 May 31;121(1):134–140. [PubMed]

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