• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jcinvestThe Journal of Clinical InvestigationCurrent IssueArchiveSubscriptionAbout the Journal
J Clin Invest. Sep 1983; 72(3): 1168–1171.
PMCID: PMC1129285

Transferable beta-lactamase. A new mechanism for in vitro penicillin resistance in Streptococcus faecalis.

Abstract

Although enterococci are relatively resistant to penicillin, the mechanism of resistance is largely unknown and enzymatic inactivation does not play a role. In this study, an isolate of Streptococcus faecalis was found to have beta lactamase activity resulting in complete inactivation of penicillin. With a high inoculum, this strain was resistant to greater than 1,000 micrograms/ml of penicillin. Penicillin resistance and beta lactamase activity were transferred by conjugation at a high frequency to an enterococcal laboratory recipient strain together with two plasmids of molecular size 34 X 10(6) and 56 X 10(6), thus demonstrating the emergence of plasmid-mediated penicillin resistance in the genus Streptococcus.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (570K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Facklam RR. Recognition of group D streptococcal species of human origin by biochemical and physiological tests. Appl Microbiol. 1972 Jun;23(6):1131–1139. [PMC free article] [PubMed]
  • Toala P, McDonald A, Wilcox C, Finland M. Susceptibility of group D streptococcus (enterococcus) to 21 antibiotics in vitro, with special reference to species differences. Am J Med Sci. 1969 Dec;258(6):416–430. [PubMed]
  • Georgopapadakou NH, Liu FY. Binding of beta-lactam antibiotics to penicillin-binding proteins of Staphylococcus aureus and Streptococcus faecalis: relation to antibacterial activity. Antimicrob Agents Chemother. 1980 Nov;18(5):834–836. [PMC free article] [PubMed]
  • Krogstad DJ, Pargwette AR. Defective killing of enterococci: a common property of antimicrobial agents acting on the cell wall. Antimicrob Agents Chemother. 1980 Jun;17(6):965–968. [PMC free article] [PubMed]
  • Robins-Brown RM, Gaspar MN, Ward JI, Wachsmuth IK, Koornhof HJ, Jacobs MR, Thornsberry C. Resistance mechanisms of multiply resistant pneumococci: antibiotic degradation studies. Antimicrob Agents Chemother. 1979 Mar;15(3):470–474. [PMC free article] [PubMed]
  • Parrillo JE, Borst GC, Mazur MH, Iannini P, Klempner MS, Moellering RC, Jr, Anderson SE. Endocarditis due to resistant viridans streptococci during oral penicillin chemoprophylaxis. N Engl J Med. 1979 Feb 8;300(6):296–300. [PubMed]
  • Lucas TJ. An evaluation of 12 methods for the demonstration of penicillinase. J Clin Pathol. 1979 Oct;32(10):1061–1065. [PMC free article] [PubMed]
  • Jacob AE, Hobbs SJ. Conjugal transfer of plasmid-borne multiple antibiotic resistance in Streptococcus faecalis var. zymogenes. J Bacteriol. 1974 Feb;117(2):360–372. [PMC free article] [PubMed]
  • Leblanc DJ, Lee LN. Rapid screening procedure for detection of plasmids in streptococci. J Bacteriol. 1979 Dec;140(3):1112–1115. [PMC free article] [PubMed]
  • Mederski-Samoraj BD, Murray BE. High-level resistance to gentamicin in clinical isolates of enterococci. J Infect Dis. 1983 Apr;147(4):751–757. [PubMed]
  • Moellering RC, Jr, Wennersten C, Weinberg AN. Synergy of penicillin and gentamicin against Enterococci. J Infect Dis. 1971 Dec;124 (Suppl):S207–S209. [PubMed]
  • Calderwood SA, Wennersten C, Moellering RC, Jr, Kunz LJ, Krogstad DJ. Resistance to six aminoglycosidic aminocyclitol antibiotics among enterococci: prevalence, evolution, and relationship to synergism with penicillin. Antimicrob Agents Chemother. 1977 Sep;12(3):401–405. [PMC free article] [PubMed]
  • Farrar WE, Jr, Gramling PK. Antistaphylococcal activity and beta-lactamase resistance of newer cephalosporins. J Infect Dis. 1976 Jun;133(6):691–695. [PubMed]
  • Sabath LD, Garner C, Wilcox C, Finland M. Effect of inoculum and of beta-lactamase on the anti-staphylococcal activity of thirteen penicillins and cephalosporins. Antimicrob Agents Chemother. 1975 Sep;8(3):344–349. [PMC free article] [PubMed]
  • Sykes RB, Matthew M. The beta-lactamases of gram-negative bacteria and their role in resistance to beta-lactam antibiotics. J Antimicrob Chemother. 1976 Jun;2(2):115–157. [PubMed]
  • Murray BE, Moellering RC., Jr Patterns and mechanisms of antibiotic resistance. Med Clin North Am. 1978 Sep;62(5):899–923. [PubMed]
  • Finland M. I. Prevalence of extrachromosomal drug resistance. Changes in asusceptibility of selected pathogenic bacteria to widely used antibiotics. Ann N Y Acad Sci. 1971 Jun 11;182:5–20. [PubMed]
  • Clewell DB. Plasmids, drug resistance, and gene transfer in the genus Streptococcus. Microbiol Rev. 1981 Sep;45(3):409–436. [PMC free article] [PubMed]
  • Engel HW, Soedirman N, Rost JA, van Leeuwen WJ, van Embden JD. Transferability of macrolide, lincomycin, and streptogramin resistances between group A, B, and D streptococci, Streptococcus pneumoniae, and Staphylococcus aureus. J Bacteriol. 1980 May;142(2):407–413. [PMC free article] [PubMed]

Articles from The Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...