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EMBO Rep. Apr 15, 2002; 3(4): 341–348.
PMCID: PMC1084057
Scientific Reports

Plk1 promotes nuclear translocation of human Cdc25C during prophase


The nuclear accumulation of active M-phase promoting factor (MPF) during prophase is thought to be essential for coordinating M-phase events in vertebrate cells. The protein phosphatase Cdc25C, an activator of MPF, enters the nucleus to keep MPF active in the nucleus during prophase. However, the molecular mechanisms that control nuclear translocation of Cdc25C during prophase are unknown. We show that phosphorylation of a serine residue (Ser198) in a nuclear export signal sequence of human Cdc25C occurs during prophase and promotes nuclear localization of Cdc25C. We also show that Polo-like kinase 1 (Plk1) is responsible for this phosphorylation and that constitutively active Plk1 promotes nuclear localization of Cdc25C. Remarkably, a mutant Cdc25C in which Ser198 is replaced by alanine remains in the cytoplasm when wild-type Cdc25C accumulates in the nucleus during prophase. These results suggest that Plk1 phosphorylates Cdc25C on Ser198 and regulates nuclear translocation of Cdc25C during prophase.


In eukaryotic cells, initiation of mitosis requires the activation of M-phase promoting factor (MPF), the complex of a cyclin-dependent kinase Cdc2 and the B-type cyclin (for reviews, see Nurse, 1990; Hunt, 1991). The kinase activity of Cdc2/cyclin-B is controlled by phosphorylation of Cdc2 and accumulation of cyclin B protein. During S and G2 phases, B-type cyclins accumulate and bind to Cdc2 to form heterodimers. Cyclin B facilitates the inhibitory phosphorylation of Cdc2 at Thr14 and Tyr15, which are catalyzed by Wee1, Mik1 and Myt1 kinases. At the end of G2, abrupt dephosphorylation of these sites by Cdc25 triggers the activation of Cdc2/cyclin-B (for reviews, see Coleman and Dunphy, 1994; Morgan, 1995; Nigg, 2001). In addition to cyclin B accumulation and phosphorylation of Cdc2, intracellular localization of cyclin B1 is also regulated during the progression of the cell cycle (for reviews, see Pines, 1999; Yang and Kornbluth, 1999). In interphase, cyclin B1 localizes to the cytoplasm due to its NES-dependent nuclear export (Hagting et al., 1998; Toyoshima et al., 1998; Yang et al., 1998). During prophase, cyclin B1 becomes phosphorylated, and the phophorylation causes nuclear translocation of cyclin B1 (Li et al., 1997; Yang et al., 1998; Hagting et al., 1999; Toyoshima-Morimoto et al., 2001; Yang et al., 2001). The nuclear accumulation of active MPF during prophase is thought to be important for initiating and coordinating M-phase events in vertebrate cells (Heald et al., 1993; Li et al., 1997; Pines, 1999; Yang and Kornbluth, 1999; Takizawa and Morgan, 2000).

However, because Wee1 is constantly nuclear (Heald et al., 1993), the nuclear MPF is inactivated unless the activator phosphatase Cdc25C counteracts the action of Wee1 (Seki et al., 1992; Heald et al., 1993; Dalal et al., 1999). Recent studies have shown that Cdc25C is cytoplasmic during interphase and accumulates in the nucleus during prophase (Seki et al., 1992; Heald et al., 1993; Dalal et al., 1999). The activity of Cdc25C is increased during G2–M phase, and this activation correlates with its hyperphosphorylation in the N-terminal portion (Izumi et al., 1992; Kumagai and Dunphy, 1992; Hoffmann et al., 1993; Izumi and Maller, 1993), which may in part be attributed to an auto-amplification loop (Hoffmann et al., 1993; Izumi and Maller, 1993; Strausfeld et al., 1994) involving Cdc2/cyclin-B. Therefore, the nuclear accumulation of Cdc25C may play an essential role in maintaining the activity of Cdc2/cyclin-B1 in the nucleus during prophase by counteracting the inhibitory activity of Wee1.

It has been shown that human Cdc25C is phosphorylated on Ser216 during interphase (Peng et al., 1997) and that this phosphorylation creates a binding site for 14-3-3 proteins (Peng et al., 1997; Kumagai et al., 1998a), which are known to bind to a phospho-Ser residue in a number of proteins and regulate their functions. C-TAK1 has been shown to be one of the kinases responsible for this phosphorylation (Peng et al., 1998). Checkpoint kinases Chk1 and Chk2 have also been shown to catalyze this phosphorylation (Furnari et al., 1997; Peng et al., 1997; Sanchez et al., 1997; Kumagai et al., 1998b; Matsuoka et al., 1998; Blasina et al., 1999). Several reports suggested that the cytoplasmic localization of Cdc25C in interphase is maintained by binding to 14-3-3 proteins (Dalal et al., 1999; Kumagai and Dunphy, 1999; Yang et al., 1999; Zeng and Piwnica-Worms, 1999) and that dephosphorylation of Ser216 occurs during M phase and blocks the interaction between Cdc25C and 14-3-3 proteins (Peng et al., 1997; Kumagai et al., 1998a). Most recently, several groups identified a nuclear export signal (NES) sequence in both Xenopus and human Cdc25C and showed that Cdc25C is exported from the nucleus by the NES-dependent transport mechanism (Kumagai and Dunphy, 1999; Yang et al., 1999; Graves et al., 2001). Thus, if Cdc25C is dissociated from 14-3-3 proteins, it should still remain in the cytoplasm. How nuclear translocation of Cdc25C during prophase is caused has not been defined. In this study, we show that phosphorylation of a serine residue (Ser198) in the NES sequence of human Cdc25C occurs during prophase and that this phosphorylation is necessary for the nuclear localization of Cdc25C. We also show that Polo-like kinase 1 (Plk1) (for reviews, see Glover et al., 1998; Nigg, 1998) is responsible for this phosphorylation. As Plk1 has been shown previously to phosphorylate cyclin B1 and induce its nuclear entry during prophase (Toyoshima-Morimoto et al., 2001), it could regulate simultaneity of nuclear entry of Cdc2/cyclin-B1 and its activator Cdc25C and thus act as an important coordinator for intracellular localization of active MPF during G2–M transition.


To examine the involvement of 14-3-3 proteins in the cytoplasmic localization of Cdc25C in interphase, we examined intracelluler localization of a mutant form of Cdc25C, in which Ser216 is replaced by Ala to eliminate the association with 14-3-3 proteins (S216A-Cdc25C) in HeLa cells. The S216A-Cdc25C, like wild-type (WT) Cdc25C, still largely localized to the cytoplasm (Figure (Figure1,1, S216A). After treatment with leptomycin B (LMB), a specific inhibitor of the NES receptor CRM1, WT-Cdc25C became localized to the nucleus, and S216A-Cdc25C strongly accumulated in the nucleus (data not shown). These results suggest that human Cdc25C, in interphase cells, is exported from the nucleus by an NES-mediated transport mechanism, which is independent of 14-3-3 proteins, and that the 14-3-3 binding may ensure cytoplasmic localization of Cdc25C by inhibiting its nuclear import, as described previously for the Xenopus system (Kumagai and Dunphy, 1999; Yang et al., 1999). We found five putative NES sequences (99-LDSSGLQEVHL-109, 190-ISDELMEFSL-199, 256-LCLKKTVSL-264, 318-FQGLIEKFYV-327 and 337-LGGHIQGALNL-347) in human Cdc25C. When HA-tagged mutant forms of human Cdc25C, in which two C-terminal hydrophobic residues in each NES sequence, in addition to Ser216, were replaced by Ala (107,109,216A-, 197,199,216A-, 262,264,216A-, 325,327,216A- and 345,347.216A-Cdc25C) were expressed in HeLa cells, only the 197,199,216A-Cdc25C accumulated in the nucleus (Figure (Figure1;1; data not shown). A mutant 197,199A-Cdc25C, in which Ser216 is not mutated, localized, but did not accumulate, to the nucleus (Figure (Figure1,1, 197,199A). When a fusion protein between GST and a polypeptide corresponding to residues 173–206 of Cdc25C [GST–Cdc25C (173–206)] was injected into the nucleus, it was exported from the nucleus in an LMB-sensitive manner (data not shown). These results indicate that the amino-acid sequence of residues 190–199 of Cdc25C (see Figure Figure2A)2A) is a functional NES. A recent report identified, using different approaches, the same portion as an NES of human Cdc25C (Graves et al., 2001).

figure kvf06901
Fig. 1. Serine phosphorylation in the NES sequence of human Cdc25C causes its nuclear localization. Indicated forms of HA-Cdc25C were expressed in HeLa cells. More than 200 cells were examined in each condition, and representative images are shown in ...
figure kvf06902
Fig. 2. Plk1 phosphorylates Ser198 of Cdc25C. (A) NES sequences of cyclin B1 (human) and Cdc25C (human). Important hydrophobic residues in NES are boxed. (B) The kinase activities of recombinant His-tagged Plk1, which was expressed in and purified from ...

Cyclin B1 has been shown to be exported from the nucleus by its NES-dependent mechanism in interphase cells (Hagting et al., 1998; Toyoshima et al., 1998; Yang et al., 1998), and phosphoylation of a serine residue (Ser147), which resides in the middle of the NES sequence (see Figure Figure2A),2A), inactivates the NES and promotes nuclear translocation of cyclin B1 during prophase (Toyoshima-Morimoto et al., 2001; Yang et al., 2001). As two serine residues (191 and 198) reside in the middle of the NES sequence of human Cdc25C, we hypothesized that phosphorylation of these serines might inactivate the NES and promote nuclear localization of Cdc25C. We constructed a mutant form of Cdc25C (S191,198E), in which both serines were replaced by Glu to mimic phosphorylation. This mutant Cdc25C became localized to the nucleus (Figure (Figure1,1, S191,198E). The nuclear localization was enhanced when Ser216 was further replaced by Ala (Figure (Figure1,1, S191,198E,S216A). Thus, it is likely that phosphorylation of Ser191 and/or Ser198 may inactivate the NES and promote the nuclear localization of Cdc25C.

Previous reports have shown that Plx1, a Xenopus homolog of Plk1, phosphorylates and activates Cdc25C (Kumagai and Dunphy, 1996). However, the phosphorylation sites have not been determined, and whether Plx1 or Plk1 regulates subcellular localization of Cdc25C has not been examined. We reported previously that Plk1 is a kinase that phosphorylates cyclin B1 on Ser147 (Toyoshima-Morimoto et al., 2001) (Figure (Figure2A).2A). The NES sequence of cyclin B1 and that of Cdc25C are both rich in hydrophobic and acidic amino-acid residues (Figure (Figure2A).2A). Our preliminary data suggest that serine residues present in such sequences could be good substrates for Plk1 (H. Nakajima, F. Toyoshima-Morimoto and E. Nishida, unpublished data). We tested whether Plk1 phosphorylates human Cdc25C on Ser191 and/or Ser198. Because Plk1 phosphorylates Cdc25C on multiple sites in the full-length Cdc25C (data not shown), we used GST–Cdc25C(173–206) as a substrate here. Recombinant His-Plk1, which was expressed in, and purified from, bacteria, phosphorylated WT-Cdc25C (173–206) but not S191,198A-Cdc25C (173–206) (Figure (Figure2B,2B, upper). S191A-Cdc25C (173–206) was phosphorylated well, but S198A-Cdc25C (173–206) was scarcely phosphorylated (Figure (Figure2B,2B, upper panel). HA-tagged Plk1, which was expressed in COS cells and purified by immunoprecipitation with anti-HA-antibody, also showed essentially the same substrate specificity (Figure (Figure2B,2B, lower panel). Moreover, His-Plk1 phosphorylated a synthetic peptide corresponding to residues 188–201 (EEISDELMEFSLKD) of Cdc25C (OVA-NES) but not a phosphopeptide (EEISDELMEF-phosphoS198-LKD, OVA-P-NES) (Figure (Figure2C).2C). To examine whether Plk1 phosphorylates full-length Cdc25C on Ser198, we produced anti-phosphoSer198 Cdc25C antibody by immunizing rabbits with the phosphoS198-NES peptide (Figure (Figure2C).2C). This antibody reacted with bacterially produced GST–WT-Cdc25C (full length), but not with GST–S198A-Cdc25C (full length), only after pre-incubation with His-Plk1 and ATP (Figure (Figure2D).2D). Taken together, these results show that Plk1 directly phosphorylates Cdc25C on Ser198.

We then examined the activity of endogenous Plk1 in synchronized HeLa cells. The kinase activity of endogenous Plk1, which was immunoprecipitated with anti-Plk1 antibody, toward WT-Cdc25C (173–206) was increased during G2–M phase, 8–10 h after release from a double thymidine block (Figure (Figure3A,3A, fourth row). Immunoprecipitated Plk1 hardly phosphorylated S198A-Cdc25C (173–206) (Figure (Figure3A,3A, fifth row). This profile coincided with the kinase activity of total lysates toward WT-Cdc25C and S198A-Cdc25C (173–206) (Figure (Figure3A,3A, first and second rows, respectively). The WT-Cdc25C (173–206)-phosphorylating activity in the extracts from M-phase HeLa cells was decreased markedly by the immunodepletion treatment with increasing amounts of anti-Plk1 antibody, but it was not decreased by the treatment with control IgG (Figure (Figure3B).3B). Bacterially produced His-Plk1 protein restored the activity of the Plk1-immunodepleted extract (see Supplementary figure 1 avaliable at EMBO reports Online). These results suggest that Plk1 is a major kinase that phosphorylates Cdc25C on Ser198 during G2–M transition.

figure kvf06903
Fig. 3. Plk1 phosphorylates Cdc25C on Ser198 during G2–M phase in HeLa cells. (A) HeLa cells were arrested at the G1–S boundary by a double thymidine block, and cell extracts were prepared at the indicated times after release. The kinase ...

To test whether phosphorylation of Ser198 could promote nuclear localization of Cdc25C, we examined the localization of S198E-Cdc25C. As shown in Figure Figure1,1, S198E-Cdc25C became localized to the nucleus. The nuclear localization was enhanced when Ser216 was further replaced by Ala (Figure (Figure1,1, S198E,S216A). Thus, phosphorylation of Ser198 could stimulate nuclear localization of Cdc25C.

Our results suggest that Plk1 phosphorylates Cdc25C on Ser198 during prophase and promotes its nuclear localization. To test this further, we expressed Plk1 with Cdc25C in HeLa cells. When co-expressed with WT-Plk1, both WT-Cdc25C and S216A-Cdc25C remained in the cytoplasm (Figure (Figure4A,4A, lanes 2 and 5). Co-expression of SDTD-Plk1, a constitutively active form of Plk1 (Qian et al., 1999), stimulated nuclear localization of S216A-Cdc25C but not WT-Cdc25C (Figure (Figure4A,4A, lanes 3 and 6). This supports the above idea. However, as the stimulatory effect was not very strong, we then examined whether Cdc2/cyclin-B1, which is able to phosphorylate Cdc25C (Hoffmann et al., 1993; Izumi and Maller, 1993; Strausfeld et al., 1994), affects nuclear localization of Cdc25C, by co-expressing cyclin B1 with Plk1. Because a high-level expression of cyclin B1 together with Cdc25C strongly induced premature mitosis (data not shown), we expressed cyclin B1 at a very low level. Expression of cyclin B1 alone or cycin B1 plus WT-Plk1 did not induce nuclear translocation of WT-Cdc25C or S216A-Cdc25C (Figure (Figure4A,4A, lanes 7, 8, 10 and 11; B, top panel). However, expression of cyclin B1 plus SDTD-Plk1 stimulated significantly nuclear localization of WT-Cdc25C (Figure (Figure4A,4A, lane 9). Expression of S216A-Cdc25C together with this low level of cyclin B1 and SDTD-Plk1 still induced a premature mitosis rather strongly under the condition (~15%, data not shown). But, under this condition also, in ~40% of the cells that were not in mitosis, S216A-Cdc25C localized to the nucleus (Figure (Figure4A,4A, lane 12; B, middle panel). This stimulation of the nuclear localization of Cdc25C was significantly reduced when Ser191 and Ser198 of Cdc25C were replaced by Ala (Figure (Figure4A,4A, lane 13; B, bottom panel). These results support our idea that Plk1 phosphorylates Cdc25C on Ser198 and stimulates its nuclear translocation during prophase. The protein level of cyclin B1 increases during G2 phase, and the increased cyclin B1 and/or Cdc2/cyclin-B1 complex may help to stimulate nuclear entry of Cdc25C. The molecular mechanism, however, is not known at present.

figure kvf06904
Fig. 4. Constitutively active Plk1 causes nuclear translocation of Cdc25C. (A) The indicated forms of Myc-tagged Cdc25C, HA-tagged Plk1 (vector, WT or SDTD) and/or untagged cyclin B1 were expressed in HeLa cells. Fifteen hours after transfection, ...

To examine whether phosphorylation of Cdc25C on Ser198 occurs in vivo, we produced and purified the anti-phosphoSer198 Cdc25C antibody (see Figure Figure2D).2D). The affinity-purified antibody (α-phosphoS198) reacted with OVA-phosphoS198-NES but not with OVA-NES (Figure (Figure5A).5A). It reacted strongly with a 55-kDa polypeptide, which co-migrated on SDS–PAGE with a lower, major band of Cdc25C (Figure (Figure5B,5B, arrow), in the M-phase extracts but not in the S-phase extracts from synchronized HeLa cells (Figure (Figure5B,5B, IB; α-phosphoS198). In addition, α-phosphoS198 immunoprecipitated the 55-kDa polypeptide of Cdc25C from M-phase extracts but not from S-phase extracts (Figure (Figure5C),5C), confirming that this antibody recognizes the Ser198-phosphorylated Cdc25C but not the Ser198-unphosphorylated Cdc25C. In synchronized HeLa cells, the phosphoSer198 Cdc25C appeared during G2–M transition, peaking at M phase, and then disappeared in anaphase/G1 phase (Figure (Figure5D,5D, IB; α-phosphoS198). This profile correlated with that of the kinase activity of immunoprecipitated endogenous Plk1 toward Cdc25C (173–206) (Figure (Figure5D,5D, lower graph). These results indicate that phosphorylation of Cdc25C on Ser198, which correlates with the activity of Plk1, occurs during G2–M phase in vivo. However, this antibody hardly recognized the slowly migrating form of Cdc25C that appeared during M phase in this type of experiment (Figure (Figure5D,5D, arrowhead). Because the slowly migrating form of Cdc25C appeared during M phase as a minor population due to the inefficiency of the synchronization by a double thymidine block, we expressed Myc-tagged WT-Cdc25C and S198A-Cdc25C in HeLa cells, arrested the cells at M phase by nocodazole treatment and then immunoprecipitated Cdc25C. The anti-phosphoSer198 Cdc25C antibody strongly reacted with the slowly migrating form of WT-Cdc25C (Figure (Figure5E,5E, lane 1) but not with that of S198A-Cdc25C (Figure (Figure5E,5E, lane 3). When the immunoprecipitates were treated with protein phosphatase PP1, they were no longer recognized by the antibody (Figure (Figure5E,5E, lanes 2 and 4, top row). These results suggest that the slowly migrating form of Cdc25C, as well as the unshifted form of Cdc25C, is phosphorylated on Ser198 during M phase and that this phosphorylation does not affect the mobility of Cdc25C on SDS–polyacrylamide gels.

figure kvf06905afigure kvf06905b
Fig. 5. Phosphorylation of Ser198 of Cdc25C occurs during G2–M phase in vivo and is necessary for its nuclear translocation. (A) Indicated amounts of OVA-NES and OVA-phosphoS198-NES were immunoblotted with the affinity-purified anti-phosphoSer198 ...

To test whether the phosphorylation of Ser198 of Cdc25C is necessary for its nuclear translocation during prophase, we co-expressed HA-tagged S198A-Cdc25C with Myc-tagged WT-Cdc25C in HeLa cells and examined their localization in prophase cells. S198A-Cdc25C remained in the cytoplasm, whereas WT-Cdc25C localized to the nucleus in prophase cells (Figure (Figure5F).5F). This indicates that the phosphorylation of Ser198 of Cdc25C during G2–M phase is necessary for its nuclear translocation.

Our results show that Plk1 phosphorylates Cdc25C on Ser198 during G2–M phase and that this phosphorylation is necessary for the nuclear translocation of Cdc25C during prophase. Our previous study showed that Plk1 phosphorylates cyclin B1 and causes its nuclear translocation during prophase (Toyoshima-Morimoto et al., 2001). Therefore, Plk1 regulates the timing of nuclear entry of both Cdc2/cyclin B1 and its activator Cdc25C. Nuclear Cdc25C plays a role in keeping nuclear MPF active by counteracting the inhibitory activity of Wee1, which is constitutively nuclear. However, as a recent report has demonstrated that Cdc25C is not necessarily essential in mice (Chen et al., 2001), Plk1 might also regulate the subcellular localization of Cdc25A and/or Cdc25B, both of which are believed to compensate for loss of Cdc25C in mice. Thus, our results suggest that Plk1 acts as an important coordinator for intracellular localization of active MPF by directing simultaneity of nuclear entry of MPF and its activator, which may be essential for coordinating M-phase events.


Mutagenesis, transfections and nocodazole treatment

Mutagenesis was performed by using the QuikChange Site-Directed Mutagenesis Kit (Stratagene). HeLa cells were transfected by using Fugene6 Transfection Reagent (Boehringer Mannheim). To arrest cells in M phase, cells were treated with 250 ng/ml nocodazole for 20 h at 18 h after transfection.

Protein preparation

Recombinant GST–Cdc25C (173–206) and GST–Cdc25C (full length) proteins were prepared by using pGEX-6P1 (Pharmacia), expressed in Escherichia coli, and purified by glutathione–Sepharose 4B (Pharmacia). His-tagged Plk1 was prepared as described previously (Toyoshima-Morimoto et al., 2001).

Immunoprecipitation, immunoblotting and cell staining

Cells were lysed in homogenizing buffer (20 mM Tris pH 7.5, 60 mM 2-glycerophosphate, 10 mM MgCl2, 10 mM NaF, 0.1% NP-40, 1 mM DTT, 1 mM Na3VO4, 1 mM PMSF, 2 µg/ml aprotinin, 1 µM okadaic acid) and centrifuged at 20 000 g for 15 min. The cell extracts were subjected to immunoblotting with anti-Plk1 antibody (Zymed) and anti-Cdc25C antibody (Santa Cruz). Endogenous Plk1 or HA-tagged Plk1 was immunoprecipitated with anti-Plk1 (Zymed) or anti-HA antibody (Santa Cruz) coupled to protein A–Sepharose or protein G–Sepharose (Pharmacia). The immunoprecipitates were further washed with homogenizing buffer and subjected to kinase assays as described below. Immunofluorescent cell staining with anti-HA (Santa Cruz or Clontech) and anti-Myc (Santa Cruz) antibodies was performed as described previously (Toyoshima et al., 1998).

Kinase assays

The cell extracts were mixed with 0.5 µg substrate, 100 µM ATP, 15 mM MgCl2 and 3 µCi [γ-32P]ATP in a final volume of 15 µl and incubated for 40 min at 25°C. Histone H1 kinase assay was conducted as described previously (Toyoshima et al., 1998). In the kinase assays for His-tagged Plk1 or immunoprecipitates, 0.1–1 µg His-tagged Plk1 or immunoprecipitates were mixed with substrate (0.3–1 µg), 50 µM ATP and 15 mM MgCl2 in a final volume of 15 µl and incubated for 20 min at 30°C in the presence or absence of 3 µCi [γ-32P]ATP. The reactions were stopped by addition of Laemmli’s sample buffer and boiling.

Treatment with protein phosphatase PP1

Myc-tagged Cdc25C was immunoprecipitated with anti-Myc antibody (Santa Cruz) from M-phase-arrested HeLa cells, as described above. Immunoprecipitates were washed with PP1 buffer (50 mM Tris pH 7.0, 0.1 mM EGTA, 5 mM DTT) and then incubated in 30 µl PP1 reaction buffer (50 mM Tris pH 7.0, 1 mM MnCl2, 0.01% Briji 35, 0.1 mM EDTA, 5 mM DTT) with 1 U PP1 (New England Biolabs) for 20 min at 30°C. The reactions were stopped by addition of Laemmli’s sample buffer and boiling.

Production of anti-Ser198-phosphorylated Cdc25C antibody (α-phosphoS198)

Anti-phosphoSer198 Cdc25C polyclonal antibody was produced in rabbits by immunizing them with a KLH-conjugated synthetic phosphopeptide corresponding to residues 188–201 of human Cdc25C (EEISDELMEF-phosphoS198-LKD). The serum was used at a 1000-fold dilution for the immunoblotting in Figure Figure5E.5E. The serum was diluted at 100-fold and loaded onto the GST–Cdc25C (full length)-conjugated glutathione–Sepharose 4B, and the unadsorbed fraction was used to detect in vitro phosphorylated GST–Cdc25C (full length) in Figure Figure2D.2D. To affinity purify the antibody, the serum was subjected to affinity chromatography on the phosphopeptide-conjugated cellulose. After several washes with TBS (20 mM Tris pH 7.5, 150 mM NaCl), proteins were eluted with 0.1 M glycine (pH 2.5), immediately neutralized by the addition of 1 M Tris pH 8.5, dialyzed against TBS and loaded onto the non-phosphopeptide (EEISDELMEFSLKD)-conjugated cellulose. The unadsorbed fractions were used for immunoblotting and immunoprecipitation. In immunoprecipitation from the extracts with α-phosphoS198, synchronized HeLa cells were lysed in lysis buffer (20 mM HEPES pH 7.5, 25 mM 2-glycerophosphate, 1.5 mM MgCl2, 2 mM EGTA, 150 mM NaCl, 50 mM NaF, 0.5% Triton X-100, 2 mM DTT, 1 mM Na3VO4, 1 mM PMSF, 2 µg/ml aprotinin) and centrifuged at 20 000 g for 15 min. α-PhosphoS198 coupled to protein A–Sepharose was added to the supernatant, and the immunoprecipitates were further washed three times with lysis buffer and then subjected to immunoblotting with anti-Cdc25C antibody.

Supplementary data

Supplementary data are available at EMBO reports Online.

Supplementary Material

Supplemetary figure 1: His-tagged Plk1 (0.1 or 0.5 g) was added to the Plk1-depleted M-phase extracts. Kinase activities toward GST-WT-Cdc25C (173-206) are shown:


We thank H. Nakajima for his technical assistance. This work was supported by grants from the Ministry of Education, Science and Culture of Japan to E.N.


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