• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of plntphysLink to Publisher's site
Plant Physiol. Jul 1992; 99(3): 817–821.
PMCID: PMC1080550

Brassica juncea Produces a Phytochelatin-Cadmium-Sulfide Complex 1

Abstract

Phytochelatins (PCs) are enzymically synthesized peptides produced in higher plants and some fungi upon exposure to heavy metals. We have examined PC production in the Se-tolerant wild mustard Brassica juncea and found that it produces two types of PC-Cd complexes with the same characteristics as those from fission yeast Schizosaccharomyces pombe, including a high molecular weight PC-Cd-sulfide form.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (779K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Abrahamson SL, Speiser DM, Ow DW. A gel electrophoresis assay for phytochelatins. Anal Biochem. 1992 Feb 1;200(2):239–243. [PubMed]
  • Dameron CT, Smith BR, Winge DR. Glutathione-coated cadmium-sulfide crystallites in Candida glabrata. J Biol Chem. 1989 Oct 15;264(29):17355–17360. [PubMed]
  • Fett WF, Dunn MF. Exopolysaccharides Produced by Phytopathogenic Pseudomonas syringae Pathovars in Infected Leaves of Susceptible Hosts. Plant Physiol. 1989 Jan;89(1):5–9. [PMC free article] [PubMed]
  • Grill E, Löffler S, Winnacker EL, Zenk MH. Phytochelatins, the heavy-metal-binding peptides of plants, are synthesized from glutathione by a specific gamma-glutamylcysteine dipeptidyl transpeptidase (phytochelatin synthase). Proc Natl Acad Sci U S A. 1989 Sep;86(18):6838–6842. [PMC free article] [PubMed]
  • Grill E, Winnacker EL, Zenk MH. Phytochelatins: the principal heavy-metal complexing peptides of higher plants. Science. 1985 Nov 8;230(4726):674–676. [PubMed]
  • Grill E, Winnacker EL, Zenk MH. Phytochelatins, a class of heavy-metal-binding peptides from plants, are functionally analogous to metallothioneins. Proc Natl Acad Sci U S A. 1987 Jan;84(2):439–443. [PMC free article] [PubMed]
  • Hamer DH. Metallothionein. Annu Rev Biochem. 1986;55:913–951. [PubMed]
  • Karin M. Metallothioneins: proteins in search of function. Cell. 1985 May;41(1):9–10. [PubMed]
  • Jackson PJ, Unkefer CJ, Doolen JA, Watt K, Robinson NJ. Poly(gamma-glutamylcysteinyl)glycine: its role in cadmium resistance in plant cells. Proc Natl Acad Sci U S A. 1987 Oct;84(19):6619–6623. [PMC free article] [PubMed]
  • Mehra RK, Garey JR, Butt TR, Gray WR, Winge DR. Candida glabrata metallothioneins. Cloning and sequence of the genes and characterization of proteins. J Biol Chem. 1989 Nov 25;264(33):19747–19753. [PubMed]
  • Mehra RK, Tarbet EB, Gray WR, Winge DR. Metal-specific synthesis of two metallothioneins and gamma-glutamyl peptides in Candida glabrata. Proc Natl Acad Sci U S A. 1988 Dec;85(23):8815–8819. [PMC free article] [PubMed]
  • Murasugi A, Wada C, Hayashi Y. Cadmium-binding peptide induced in fission yeast, Schizosaccharomyces pombe. J Biochem. 1981 Nov;90(5):1561–1564. [PubMed]
  • Murasugi A, Wada C, Hayashi Y. Purification and unique properties in UV and CD spectra of Cd-binding peptide 1 from Schizosaccharomyces pombe. Biochem Biophys Res Commun. 1981 Dec 15;103(3):1021–1028. [PubMed]
  • Murasugi A, Wada C, Hayashi Y. Occurrence of acid-labile sulfide in cadmium-binding peptide 1 from fission yeast. J Biochem. 1983 Feb;93(2):661–664. [PubMed]
  • Mutoh N, Hayashi Y. Isolation of mutants of Schizosaccharomyces pombe unable to synthesize cadystin, small cadmium-binding peptides. Biochem Biophys Res Commun. 1988 Feb 29;151(1):32–39. [PubMed]
  • Rabinowitz JC. Analysis of acid-labile sulfide and sulfhydryl groups. Methods Enzymol. 1978;53:275–277. [PubMed]
  • Rauser WE. Phytochelatins. Annu Rev Biochem. 1990;59:61–86. [PubMed]
  • Reese RN, Mehra RK, Tarbet EB, Winge DR. Studies on the gamma-glutamyl Cu-binding peptide from Schizosaccharomyces pombe. J Biol Chem. 1988 Mar 25;263(9):4186–4192. [PubMed]
  • Reese RN, White CA, Winge DR. Cadmium-Sulfide Crystallites in Cd-(gammaEC)(n)G Peptide Complexes from Tomato. Plant Physiol. 1992 Jan;98(1):225–229. [PMC free article] [PubMed]
  • Reese RN, Winge DR. Sulfide stabilization of the cadmium-gamma-glutamyl peptide complex of Schizosaccharomyces pombe. J Biol Chem. 1988 Sep 15;263(26):12832–12835. [PubMed]
  • Scheller HV, Huang B, Hatch E, Goldsbrough PB. Phytochelatin synthesis and glutathione levels in response to heavy metals in tomato cells. Plant Physiol. 1987 Dec;85(4):1031–1035. [PMC free article] [PubMed]
  • Steffens JC, Hunt DF, Williams BG. Accumulation of non-protein metal-binding polypeptides (gamma-glutamyl-cysteinyl)n-glycine in selected cadmium-resistant tomato cells. J Biol Chem. 1986 Oct 25;261(30):13879–13882. [PubMed]

Articles from Plant Physiology are provided here courtesy of American Society of Plant Biologists

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...