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Plant Physiol. 1992 April; 98(4): 1290–1297. | PMCID: PMC1080347 |
Interactions of Avocado (Persea americana) Cytochrome P-450 with Monoterpenoids David L. Hallahan, Jonathan H. A. Nugent, Beverly J. Hallahan, Glenn W. Dawson, Diane W. Smiley, Jevon M. West, and Roger M. Wallsgrove Biochemistry and Physiology Department, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Herts. AL5 2JQ, United Kingdom Insecticides and Fungicides Department, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Herts. AL5 2JQ, United Kingdom Department of Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom Abstract The microsomal fraction of avocado (Persea americana) mesocarp is a rich source of cytochrome P-450 active in the demethylation of xenobiotics. Cytochrome P-450 from this tissue has been purified and well characterized at the molecular level (DP O'Keefe, KJ Leto [1989] Plant Physiol 89: 1141-1149; KR Bozak, H Yu, R Sirevag, RE Christoffersen [1990] Proc Natl Acad Sci USA 87: 3904-3908). Despite this extensive characterization, the role of the enzyme in vivo was not established. Optical and electron paramagnetic resonance binding studies described here suggest that the monoterpenoids, nerol and geraniol, are substrates of avocado cytochrome P-450 (spectral dissociation constant of 7.2 and 35 micromolar, respectively). Avocado microsomes have been shown to catalyze the hydroxylation of these monoterpenoids, and both nerol and geraniol have been shown to inhibit the activity of avocado cytochrome P-450 toward the artificial substrate 7-ethoxycoumarin, with nerol a competitive inhibitor of this activity. Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.2M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References. These references are in PubMed. This may not be the complete list of references from this article. - Bozak KR, Yu H, Sirevåg R, Christoffersen RE. Sequence analysis of ripening-related cytochrome P-450 cDNAs from avocado fruit. Proc Natl Acad Sci U S A. 1990 May;87(10):3904–3908. [PMC free article] [PubMed]
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