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Plant Physiol. Nov 1984; 76(3): 658–663.
PMCID: PMC1064351

Nitrogen Metabolism of the Marine Microalga Chlorella autotrophica1


The levels of glutamine synthetase (GS) and glutamate dehydrogenase (GDH) in Chlorella autotrophica (clone 580) are strongly regulated by the nitrogen source and salt concentration of the medium. GS is present at high levels in NO3-grown cells, and at maximum levels in nitrogen-starved cells. However, the levels of GS in these cells are somewhat decreased by increasing salinity. Cells growing on NH4+ have high NADPH-GDH activity, the levels of which increase with increasing NH4+ supply, while GS decreases to a very low level under these conditions. Salinity intensifies the induction of NADPH-GDH activity in NH4+-grown cells. The levels of NADH-GDH are low in this alga, but present under all growth conditions. Methionine sulfoximine (MSX) has little effect on growth and nitrogen assimilation of the alga in the presence of NH4+.

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Selected References

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  • Ahmad I, Hellebust JA. Osmoregulation in the Extremely Euryhaline Marine Micro-Alga Chlorella autotrophica. Plant Physiol. 1984 Apr;74(4):1010–1015. [PMC free article] [PubMed]
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  • Lea PJ, Miflin BJ. The occurrence of glutamate synthase in algae. Biochem Biophys Res Commun. 1975 Jan 2;64(3):856–862. [PubMed]
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  • Molin WT, Cunningham TP, Bascomb NF, White LH, Schmidt RR. Light requirement for induction and continuous accumulation of an ammonium-inducible NADP-specific glutamate dehydrogenase in chlorella. Plant Physiol. 1981 Jun;67(6):1250–1254. [PMC free article] [PubMed]
  • Shatilov VR, Kretovich WL. Glutamate dehydrogenases from Chlorella: forms, regulation and properties. Mol Cell Biochem. 1977 May 3;15(3):201–212. [PubMed]
  • Sumar N, Casselton PJ, McNally SF, Stewart GR. Occurrence of Isóenzymes of Glutamine Synthetase in the Alga Chlorella kessleri. Plant Physiol. 1984 Feb;74(2):204–207. [PMC free article] [PubMed]
  • Talley DJ, White LH, Schmidt RR. Evidence for NADH- and NADPH-specific isozymes of glutamate dehydrogenase and the continuous inducibility of the NADPH-specific isozyme throughout the cell cycle of the eucaryote Chlorella. J Biol Chem. 1972 Dec 25;247(24):7927–7935. [PubMed]

Articles from Plant Physiology are provided here courtesy of American Society of Plant Biologists


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