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Plant Physiol. Jun 1990; 93(2): 829–832.
PMCID: PMC1062592

Expression of cab Genes in Douglas-Fir Is Not Strongly Regulated by Light 1

Abstract

Dark-grown Douglas-fir (Pseudotsuga menziesii [Mirb.] Franco) seedlings had approximately 30% of the major polypeptide of the light-harvesting chlorophyll a/b binding protein, 30% of cab mRNA, 54% of psbA mRNA, and 14% of total chlorophyll, in comparison with amounts in light-grown seedlings. Seedlings entrained under a 24-hour photoperiod of light and dark showed small diurnal fluctuations in cab and psbA mRNA levels and, when transferred to continuous conditions, no circadian rhythms in mRNA levels were apparent. These results suggest that regulation of cab gene expression in Douglas-fir differs from regulation in angiosperms, because in the latter, both light and circadian factors strongly influence the expression of cab genes.

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Selected References

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  • Arnon DI. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. [PMC free article] [PubMed]
  • Bedbrook JR, Link G, Coen DM, Bogorad L. Maize plastid gene expressed during photoregulated development. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3060–3064. [PMC free article] [PubMed]
  • Dunsmuir P, Smith SM, Bedbrook J. The major chlorophyll a/b binding protein of petunia is composed of several polypeptides encoded by a number of distinct nuclear genes. J Mol Appl Genet. 1983;2(3):285–300. [PubMed]
  • Feinberg AP, Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Simpson J, VAN Montagu M, Herrera-Estrella L. Photosynthesis-associated gene families: differences in response to tissue-specific and environmental factors. Science. 1986 Jul 4;233(4759):34–38. [PubMed]
  • Taylor WC. Transcriptional regulation by a circadian rhythm. Plant Cell. 1989 Feb;1(2):259–264. [PMC free article] [PubMed]
  • Yamamoto N, Matsuoka M, Kano-Murakami Y, Tanaka Y, Ohashi Y. Nucleotide sequence of a full length cDNA clone of light harvesting chlorophyll a/b binding protein gene from green dark-grown pine (Pinus tunbergii) seedling. Nucleic Acids Res. 1988 Dec 23;16(24):11829–11829. [PMC free article] [PubMed]

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