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Appl Microbiol. Mar 1965; 13(2): 232–235.
PMCID: PMC1058227

Evaluation of Live Tularemia Vaccine Prepared in a Chemically Defined Medium


A chemically defined medium was prepared which adequately supported growth of a vaccine strain of Pasteurella tularensis. This medium differed from those previously described in: (i) concentration of components, (ii) a requirement for calcium pantothenate to obtain increased growth, and (iii) a low initial pH. Varying the concentration of individual components up to 10 times the standard amount did not increase the viable population or affect dissociation. The vaccine strain grown in this chemically defined medium, although lower in viable population, appears to retain its identity and to be equal in potency to that prepared by the conventional method. This preliminary study indicates the potential utility of this medium as a basis for controlled studies of a live bacterial vaccine in terms of growth characteristics, dissociation, virulence, and immunogenicity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • EIGELSBACH HT, DOWNS CM. Prophylactic effectiveness of live and killed tularemia vaccines. I. Production of vaccine and evaluation in the white mouse and guinea pig. J Immunol. 1961 Oct;87:415–425. [PubMed]
  • MAGER J, TRAUB A, GROSSOWK ZN. Cultivation of Pasteurella tularensis in chemically defined media: effect of buffers and spermine. Nature. 1954 Oct 16;174(4433):747–748. [PubMed]
  • TRAUB A, MAGER J, GROSSOWICZ N. Studies on the nutrition of Pasteurella tularensis. J Bacteriol. 1955 Jul;70(1):60–69. [PMC free article] [PubMed]
  • NAGLE SC, Jr, ANDERSON RE, GARY ND. Chemically defined medium for the growth of Pasteurella tularensis. J Bacteriol. 1960 Apr;79:566–571. [PMC free article] [PubMed]
  • Downs CM, Coriell LL, Chapman SS, Klauber A. The Cultivation of Bacterium tularense in Embryonated Eggs. J Bacteriol. 1947 Jan;53(1):89–100. [PMC free article] [PubMed]

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