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Plant Physiol. May 1987; 84(1): 73–77.
PMCID: PMC1056530

Effects of Cold Hardening on the Regulation of Polyamine Levels in Wheat (Triticum aestivum L.) and Alfalfa (Medicago sativa L.) 1


When leaves of wheat (Triticum aestivum L.) are exposed to a cold hardening temperature, a major accumulation of putrescine (6-9 times) takes place. Spermidine accumulates to a lesser extent and, conversely, spermine decreases slightly. These variations are completely reversible when plants are returned to initial growing conditions. A similar response is obtained with crowns. During cold hardening, arginine decarboxylase activity remains near its initial level while a considerable loss of activity is observed in control plants. Ornithine decarboxylase and diamine oxidase activity levels are not substantially modified by the treatment. Alfalfa (Medicago sativa L.) also accumulates putrescine under low temperature stress, indicating that this phenomenon is not typical of cereals. The physiological significance of this accumulation of putrescine is still unexplained but the results obtained suggest the involvement of polyamines in the biochemical processes of cold hardening.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Dai YR, Kaur-Sawhney R, Galston AW. Promotion by gibberellic Acid of polyamine biosynthesis in internodes of light-grown dwarf peas. Plant Physiol. 1982 Jan;69(1):103–106. [PMC free article] [PubMed]
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  • Siminovitch D, Rheaume B, Pomeroy K, Lepage M. Phospholipid, protein, and nucleic acid increases in protoplasm and membrane structures associated with development of extreme freezing resistance in black locust tree cells. Cryobiology. 1968 Nov-Dec;5(3):202–225. [PubMed]
  • Slocum RD, Kaur-Sawhney R, Galston AW. The physiology and biochemistry of polyamines in plants. Arch Biochem Biophys. 1984 Dec;235(2):283–303. [PubMed]
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Articles from Plant Physiology are provided here courtesy of American Society of Plant Biologists


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