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Journal List > Plant Physiol > v.89(1); Jan 1989

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Plant Physiol. 1989 January; 89(1): 381–385.
PMCID: PMC1055847
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Immunogold Localization of Photosynthetic Fructose-1,6-Bisphosphatase in Pea Leaf Tissue 1
Rosario Hermoso, María R. de Felipe, Amparo Vivó, Ana Chueca, Juan J. Lázaro, and Julio L. Gorge
Department of Plant Biochemistry, Estación Experimental del Zaidín (CSIC), 18008 Granada, Spain
Department of Plant Biology, Instituto de Edafología y Biología Vegetal (CSIC), 28006 Madrid, Spain
1 Supported by a grant from Comisión Asesora de Investigación Científica y Técnica (Spain).
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Abstract
An enriched IgG serum fraction obtained from rabbits immunized against pea chloroplast fructose-1,6-bisphosphatase (FBPase) was used, coupled to colloidal gold (15 nanometer particles) goat anti-rabbit IgG, to analyze by electron microscopy the location of photosynthetic FBPase in pea (Pisum sativum L.) leaf ultrathin sections. In accordance with earlier biochemical studies on distribution of FBPase activity, the enzyme was visualized both in the stromal space and bound to the chloroplast membranes. Some gold particles also appear in the cytoplasm, which can be related to the presence in the cytosol of a high molecular weight precursor of this nuclear coded enzyme.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
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