Logo of plntphysLink to Publisher's site
Plant Physiol. Feb 1988; 86(2): 540–546.
PMCID: PMC1054520

Two Different Families of Hydroxyproline-Rich Glycoproteins in Melon Callus

Biochemical and Immunochemical Studies


Two different families of hydroxyproline-rich glycoproteins, HRGP1 and HRGP2, have been isolated from melon callus and separated by ion exchange chromatography on CM-sepharose. HRGP1 corresponds to an arabinogalactan protein. The sugar portion of HRGP1 accounts for 94% of the molecule and contains galactose (66%) and arabinose (34%); these residues are present as polysaccharide side chains attached to hydroxyproline. Hydroxyproline is the main amino acid residue (46%) of the protein moiety. The arabinogalactan protein nature of HRGP1 has been checked by its ability to positively react with the β-glucosyl Yariv antigen; the 3H-labeled deglycosylated HRGP1 also called HRP1 migrates upon electrophoresis as a single band of molecular weight 76,000. HRGP2 was fractionated by affinity chromatography on heparin-Ultrogel into three different glycoproteins, HRGP2a,2b and 2c. Two of these glycoproteins behave as polycations (HRGP2b and 2c) and are chemically distinct from HRGP2a. HRGP2b is the most abundant component and contains 41% protein and 50% sugar. Hydroxyproline, lysine, tyrosine, and arabinose are the most prominent residues of their respective moiety. The glycosylation pattern of hydroxyproline indicates that HRGP2b is related to and possibly a precursor of the wall HRGP; as in melon cell wall HRGP, Hyp-Ara3 predominates, and small amounts of a putative Hyp-Ara5 a hitherto unreported hyp-arabinoside, are recorded. The molecular weight of HRP2b, the protein portion of HRGP2b is 55,000 ± 5,000, as estimated after deglycosylation of the molecule with trifluoromethane sulfonic acid. Antibodies have been raised against HRGP2b and HRP2b. Immunodiffusion shows that each antigen (HRGP2b or HRP2b) reacts with its own IgG, and cross-reacts with the heterologous IgG, thereby indicating the presence of common (unglycosylated) and specific (glycosylated and deglycosylated) epitopes. The arabinogalactan protein HRGP1 is not recognized by either antibody and HRGP2b does not react with the Yariv antigen. Immunoprecipitation of 3H-labeled HRP1 and HRP2b in the presence of goat antirabbit IgG, followed by gel electrophoresis, allows to recover HRP2b only. Again, HRP2b is immunoprecipitated by the two antisera.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.8M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bonner WM, Laskey RA. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. [PubMed]
  • Cassab GI, Nieto-Sotelo J, Cooper JB, van Holst GJ, Varner JE. A developmentally regulated hydroxyproline-rich glycoprotein from the cell walls of soybean seed coats. Plant Physiol. 1985 Mar;77(3):532–535. [PMC free article] [PubMed]
  • Chrispeels MJ. Synthesis and secretion of hydroxyproline containing macromolecules in carrots. I. Kinetic analysis. Plant Physiol. 1969 Aug;44(8):1187–1193. [PMC free article] [PubMed]
  • Edge AS, Faltynek CR, Hof L, Reichert LE, Jr, Weber P. Deglycosylation of glycoproteins by trifluoromethanesulfonic acid. Anal Biochem. 1981 Nov 15;118(1):131–137. [PubMed]
  • Esquerré-Tugayé MT, Lafitte C, Mazau D, Toppan A, Touzé A. Cell Surfaces in Plant-Microorganism Interactions: II. Evidence for the Accumulation of Hydroxyproline-rich Glycoproteins in the Cell Wall of Diseased Plants as a Defense Mechanism. Plant Physiol. 1979 Aug;64(2):320–326. [PMC free article] [PubMed]
  • Heyns W, Bossyns D, Peeters B, Rombauts W. Study of a proline-rich polypeptide bound to the prostatic binding protein of rat ventral prostate. J Biol Chem. 1982 Jul 10;257(13):7407–7413. [PubMed]
  • Kumarasamy R, Symons RH. The tritium labeling of small amounts of protein for analysis by electrophoresis on sodium dodecyl sulfate--polyacrylamide slab gels. Anal Biochem. 1979 Jun;95(2):359–363. [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Lamport DT, Miller DH. Hydroxyproline arabinosides in the plant kingdom. Plant Physiol. 1971 Oct;48(4):454–456. [PMC free article] [PubMed]
  • Leach JE, Cantrell MA, Sequeira L. Hydroxyproline-rich bacterial agglutinin from potato : extraction, purification, and characterization. Plant Physiol. 1982 Nov;70(5):1353–1358. [PMC free article] [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • Mellon JE, Helgeson JP. Interaction of a hydroxyproline-rich glycoprotein from tobacco callus with potential pathogens. Plant Physiol. 1982 Aug;70(2):401–405. [PMC free article] [PubMed]
  • Muenzer J, Bildstein C, Gleason M, Carlson DM. Properties of proline-rich proteins from parotid glands of isoproterenol-treated rats. J Biol Chem. 1979 Jul 10;254(13):5629–5634. [PubMed]
  • Roby D, Toppan A, Esquerré-Tugayé MT. Cell surfaces in plant-microorganism interactions : v. Elicitors of fungal and of plant origin trigger the synthesis of ethylene and of cell wall hydroxyproline-rich glycoprotein in plants. Plant Physiol. 1985 Mar;77(3):700–704. [PMC free article] [PubMed]
  • Smith MA. Characterization of Carrot Cell Wall Protein : II. IMMUNOLOGICAL STUDY OF CELL WALL PROTEIN. Plant Physiol. 1981 Oct;68(4):964–968. [PMC free article] [PubMed]
  • Stuart DA, Varner JE. Purification and Characterization of a Salt-extractable Hydroxyproline-rich Glycoprotein from Aerated Carrot Discs. Plant Physiol. 1980 Nov;66(5):787–792. [PMC free article] [PubMed]
  • van Holst GJ, Varner JE. Reinforced Polyproline II Conformation in a Hydroxyproline-Rich Cell Wall Glycoprotein from Carrot Root. Plant Physiol. 1984 Feb;74(2):247–251. [PMC free article] [PubMed]
  • van Holst GJ, Clarke AE. Quantification of arabinogalactan-protein in plant extracts by single radial gel diffusion. Anal Biochem. 1985 Aug 1;148(2):446–450. [PubMed]
  • Ziemer MA, Mason A, Carlson DM. Cell-free translations of proline-rich protein mRNAs. J Biol Chem. 1982 Sep 25;257(18):11176–11180. [PubMed]

Articles from Plant Physiology are provided here courtesy of American Society of Plant Biologists


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • Compound
    PubChem Compound links
  • MedGen
    Related information in MedGen
  • PubMed
    PubMed citations for these articles
  • Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...