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Br J Ophthalmol. Mar 1980; 64(3): 164–169.
PMCID: PMC1039380

Corneal endothelial changes under induced intraocular pressure elevation: a scanning and transmission electron microscopic study in rabbits.


Intraocular pressure was artificially raised to 60--70 mmHg in 7 albino rabbits for periods of 15 minutes to 4 hours. The corneal endothelium of these eyes was studied by transmission and scanning electron microscopy. A correlation between exposure time to elevated IOP, clinical signs observed by slit-lamp examination, and extent of morphological damage is clearly shown. In eyes exposed to high pressure for 15 and 30 minutes corneas remained transparent and only minimal changes could be detected by SEM, which consisted of small areas of cell with unevenness of their surface, occasional cellular ruptures, and diminution of cilia and microvilli. After 1--2 hours of exposure small, solitary corneal opacifications appeared. In these eyes more severe morphological changes affecting larger areas were observed, with additional cellular blebbing, excariocytosis, cellular rupture, disintegration, and disappearance seen in SEM. Thin sections revealed swelling of mitochondria, disorganisation of endoplasmic reticulum, and the existence of myelin bodies. In eyes exposed for 3 and 4 hours to high IOP corneal haziness, implying stromal oedema, appeared. In these eyes the areas affected were larger, the extent of damage being more severe. Many areas were bare of endothelium, surrounded by scattered cellular debris, and showed cells with ballooning surfaces and multiple ruptures. Even in severe cellular damage cellular junctions appeared intact. It is assumed that endothelial cells are more sensitive to IOP elevation than the cellular junctions and that injury to the active pump system due to morphological damage is responsible for the resultant corneal oedema.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Ytteborg J, Dohlman CH. Corneal edema and intraocular pressure. II. Clinical results. Arch Ophthalmol. 1965 Oct;74(4):477–484. [PubMed]
  • Donn A. Cornea and sclera. Arch Ophthalmol. 1966 Feb;75(2):261–288. [PubMed]
  • Fischbarg J. Active and passive properties of the rabbit corneal endothelium. Exp Eye Res. 1973 May 10;15(5):615–638. [PubMed]
  • Kaye GI, Sibley RC, Hoefle FB. Recent studies on the nature and function of the corneal endothelial barrier. Exp Eye Res. 1973 May 10;15(5):585–613. [PubMed]
  • IRVINE AR., Jr The role of the endothelium in bullous keratopathy. AMA Arch Ophthalmol. 1956 Sep;56(3):338–351. [PubMed]
  • McCarey BE, Edelhauser HF, Van Horn DL. Functional and structural changes in the corneal edothelium during in vitro perfusion. Invest Ophthalmol. 1973 Jun;12(6):410–417. [PubMed]
  • Blümcke S, Morgenroth K., Jr The stereo ultrastructure of the external and internal surface of the cornea. J Ultrastruct Res. 1967 Jun;18(5):502–518. [PubMed]
  • Svedbergh B, Bill A. Scanning electron microscopic studies of the corneal endothelium in man and monkeys. Acta Ophthalmol (Copenh) 1972;50(3):321–336. [PubMed]
  • Doughman DJ, Van Horn D, Harris JE, Miller GE, Lindstrom R, Good RA. The ultrastructure of human organ-cultured cornea. I. Endothelium. Arch Ophthalmol. 1974 Dec;92(6):516–523. [PubMed]
  • Trenberth SM, Mishima S. The effect of ouabain on the rabbit corneal endothelium. Invest Ophthalmol. 1968 Feb;7(1):44–52. [PubMed]
  • Kaye GI, Mishima S, Cole JD, Kaye NW. Studies on the cornea. VII. Effects of perfusion with a Ca++-free medium on the corneal endothelium. Invest Ophthalmol. 1968 Feb;7(1):53–66. [PubMed]
  • Kaye GI, Hoefle FB, Donn A. Studies on the cornea. 8. Reversibility of the effects of in vitro perfusion of the rabbit corneal endothelium with calcium-free medium. Invest Ophthalmol. 1973 Feb;12(2):98–113. [PubMed]
  • KAYE GI, PAPPAS GD, DONN A, MALLETT N. Studies on the cornea. II. The uptake and transport of colloidal particles by the living rabbit cornea in vitro. J Cell Biol. 1962 Mar;12:481–501. [PMC free article] [PubMed]

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