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Arch Dis Child. Nov 1993; 69(5 Spec No): 483–487.
PMCID: PMC1029589

Enteral feeding of premature infants with Lactobacillus GG.


The objectives of this study were to determine whether or not the probiotic Lactobacillus GG can colonise the immature bowel of premature infants and if so, does colonisation result in a reduction of the size of the bowel reservoir of nosocomial pathogens such as enterobacteriaceae, enterococci, yeasts or staphylococci, and does colonisation with Lactobacillus GG have any effect on the clinical progress and outcome. Twenty preterm infants with a gestational age of 33 weeks or less who were resident on a neonatal unit were studied from the initiation of milk feeds until discharge. The infants were randomised to receive either milk feeds or milk feeds supplemented with Lactobacillus GG 10(8) colony forming units twice a day for two weeks. The clinical features of the two groups of infants were similar. Orally administered Lactobacillus GG was well tolerated and did colonise the bowel of premature infants. However, colonisation with Lactobacillus GG did not reduce the faecal reservoir of potential pathogens and there was no evidence that colonisation had any positive clinical benefit for this particular group of infants.

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Selected References

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  • Bennet R, Eriksson M, Nord CE, Zetterström R. Fecal bacterial microflora of newborn infants during intensive care management and treatment with five antibiotic regimens. Pediatr Infect Dis. 1986 Sep-Oct;5(5):533–539. [PubMed]
  • Sakata H, Yoshioka H, Fujita K. Development of the intestinal flora in very low birth weight infants compared to normal full-term newborns. Eur J Pediatr. 1985 Jul;144(2):186–190. [PubMed]
  • Hall MA, Cole CB, Smith SL, Fuller R, Rolles CJ. Factors influencing the presence of faecal lactobacilli in early infancy. Arch Dis Child. 1990 Feb;65(2):185–188. [PMC free article] [PubMed]
  • Goldmann DA, Leclair J, Macone A. Bacterial colonization of neonates admitted to an intensive care environment. J Pediatr. 1978 Aug;93(2):288–293. [PubMed]
  • Millar MR, MacKay P, Levene M, Langdale V, Martin C. Enterobacteriaceae and neonatal necrotising enterocolitis. Arch Dis Child. 1992 Jan;67(1 Spec No):53–56. [PMC free article] [PubMed]
  • Hoy C, Millar MR, MacKay P, Godwin PG, Langdale V, Levene MI. Quantitative changes in faecal microflora preceding necrotising enterocolitis in premature neonates. Arch Dis Child. 1990 Oct;65(10 Spec No):1057–1059. [PMC free article] [PubMed]
  • Fuller R. Probiotics in man and animals. J Appl Bacteriol. 1989 May;66(5):365–378. [PubMed]
  • Goldmann DA. The bacterial flora of neonates in intensive care-monitoring and manipulation. J Hosp Infect. 1988 Feb;11 (Suppl A):340–351. [PubMed]
  • Goldin BR, Gorbach SL, Saxelin M, Barakat S, Gualtieri L, Salminen S. Survival of Lactobacillus species (strain GG) in human gastrointestinal tract. Dig Dis Sci. 1992 Jan;37(1):121–128. [PubMed]
  • Silva M, Jacobus NV, Deneke C, Gorbach SL. Antimicrobial substance from a human Lactobacillus strain. Antimicrob Agents Chemother. 1987 Aug;31(8):1231–1233. [PMC free article] [PubMed]
  • Talarico TL, Casas IA, Chung TC, Dobrogosz WJ. Production and isolation of reuterin, a growth inhibitor produced by Lactobacillus reuteri. Antimicrob Agents Chemother. 1988 Dec;32(12):1854–1858. [PMC free article] [PubMed]
  • Reid G, Bruce AW, McGroarty JA, Cheng KJ, Costerton JW. Is there a role for lactobacilli in prevention of urogenital and intestinal infections? Clin Microbiol Rev. 1990 Oct;3(4):335–344. [PMC free article] [PubMed]
  • Juven BJ, Meinersmann RJ, Stern NJ. Antagonistic effects of lactobacilli and pediococci to control intestinal colonization by human enteropathogens in live poultry. J Appl Bacteriol. 1991 Feb;70(2):95–103. [PubMed]
  • Gorbach SL, Chang TW, Goldin B. Successful treatment of relapsing Clostridium difficile colitis with Lactobacillus GG. Lancet. 1987 Dec 26;2(8574):1519–1519. [PubMed]
  • Oksanen PJ, Salminen S, Saxelin M, Hämäläinen P, Ihantola-Vormisto A, Muurasniemi-Isoviita L, Nikkari S, Oksanen T, Pörsti I, Salminen E, et al. Prevention of travellers' diarrhoea by Lactobacillus GG. Ann Med. 1990 Feb;22(1):53–56. [PubMed]
  • Isolauri E, Juntunen M, Rautanen T, Sillanaukee P, Koivula T. A human Lactobacillus strain (Lactobacillus casei sp strain GG) promotes recovery from acute diarrhea in children. Pediatrics. 1991 Jul;88(1):90–97. [PubMed]
  • Bouhnik Y, Pochart P, Marteau P, Arlet G, Goderel I, Rambaud JC. Fecal recovery in humans of viable Bifidobacterium sp ingested in fermented milk. Gastroenterology. 1992 Mar;102(3):875–878. [PubMed]
  • Marteau P, Pochart P, Bouhnik Y, Zidi S, Goderel I, Rambaud JC. Survie, dans l'intestin grêle, de Lactobacillus acidophilus et Bifidobacterium sp, ingerés dans un lait fermenté. Une base rationnelle à l'utilisation de probiotiques chez l'homme. Gastroenterol Clin Biol. 1992;16(1):25–28. [PubMed]
  • Clements ML, Levine MM, Ristaino PA, Daya VE, Hughes TP. Exogenous lactobacilli fed to man - their fate and ability to prevent diarrheal disease. Prog Food Nutr Sci. 1983;7(3-4):29–37. [PubMed]
  • Smith HW. Survival of orally administered E. coli K 12 in alimentary tract of man. Nature. 1975 Jun 5;255(5508):500–502. [PubMed]
  • Reuman PD, Duckworth DH, Smith KL, Kagan R, Bucciarelli RL, Ayoub EM. Lack of effect of Lactobacillus on gastrointestinal bacterial colonization in premature infants. Pediatr Infect Dis. 1986 Nov-Dec;5(6):663–668. [PubMed]
  • Stansbridge EM, Walker V, Hall MA, Smith SL, Millar MR, Bacon C, Chen S. Effects of feeding premature infants with Lactobacillus GG on gut fermentation. Arch Dis Child. 1993 Nov;69(5 Spec No):488–492. [PMC free article] [PubMed]
  • Itoh K, Freter R. Control of Escherichia coli populations by a combination of indigenous clostridia and lactobacilli in gnotobiotic mice and continuous-flow cultures. Infect Immun. 1989 Feb;57(2):559–565. [PMC free article] [PubMed]

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