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Ann Rheum Dis. Apr 1977; 36(2): 121–129.
PMCID: PMC1006646

Chemical composition and swelling of normal and osteoarthrotic femoral head cartilage. I. Chemical composition.


Radiochemical and biochemical methods were used to characterize post-mortem and osteoarthrotic femoral head cartilage. Fixed charge density measurements were correlated with glycosaminoglycan content as estimated by uronic acid and hexosamine analyses. In post-mortem cartilage water content decreased from a maximum at the surface to a minimum in the deep zones. In the osteoarthrotic specimens water content was greatest in the middle zones. Glycosaminoglycan content increased with depth and in the osteoarthrotic specimens was reduced throughout the depth of the cartilage. With increasing degeneration there was an increase in water content and decrease in glycosaminoglycan content. The difference in the water content profile in osteoarthrotic cartilage was explained in terms of damage to the collagen network. In osteoarthrosis the latter is no longer capable of restraining the swelling pressure produced by the glycosaminoglycans and swelling is greatest in the midzones, where glycosaminoglycan content is highest.

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Selected References

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  • BITTER T, MUIR HM. A modified uronic acid carbazole reaction. Anal Biochem. 1962 Oct;4:330–334. [PubMed]
  • Bollet AJ, Nance JL. Biochemical Findings in Normal and Osteoarthritic Articular Cartilage. II. Chondroitin Sulfate Concentration and Chain Length, Water, and Ash Content. J Clin Invest. 1966 Jul;45(7):1170–1177. [PMC free article] [PubMed]
  • BOLLET AJ, HANDY JR, STURGILL BC. Chondroitin sulfate concentration and protein-polysaccharide composition of articular cartilage in osteoarthritis. J Clin Invest. 1963 Jun;42:853–859. [PMC free article] [PubMed]
  • Elson LA, Morgan WT. A colorimetric method for the determination of glucosamine and chondrosamine. Biochem J. 1933;27(6):1824–1828. [PMC free article] [PubMed]
  • Ficat C, Maroudas A. Cartilage of the patella. Topographical variation of glycosaminoglycan content in normal and fibrillated tissue. Ann Rheum Dis. 1975 Dec;34(6):515–519. [PMC free article] [PubMed]
  • GRANT RA. ESTIMATION OF HYDROXYPROLINE BY THE AUTOANALYSER. J Clin Pathol. 1964 Nov;17:685–686. [PMC free article] [PubMed]
  • Hjertquist SO, Lemperg R. Studies of autologous diced costal cartilage transplant. 3. With special regard to glycosaminoglycans, hydroxyproline, calcium and 35S-sulphate incorporation in vitro after intramuscular implantation. Acta Soc Med Ups. 1967;72(3):173–198. [PubMed]
  • Hjertquist SO, Lemperg R. Identification and concentration of the glycosaminoglycans of human articular cartilage in relation to age and osteoarthritis. Calcif Tissue Res. 1972;10(3):223–237. [PubMed]
  • Lemperg R, Larsson SE, Hjertquist SO. The glycosaminoglycans of bovine articular cartilage. I. Concentration and distribution in different layers in relation to age. Calcif Tissue Res. 1974;15(3):237–251. [PubMed]
  • McDevitt CA, Muir H. Biochemical changes in the cartilage of the knee in experimental and natural osteoarthritis in the dog. J Bone Joint Surg Br. 1976 Feb;58(1):94–101. [PubMed]
  • Mankin HJ, Lippiello L. The glycosaminoglycans of normal and arthritic cartilage. J Clin Invest. 1971 Aug;50(8):1712–1719. [PMC free article] [PubMed]
  • Maroudas A. Distribution and diffusion of solutes in articular cartilage. Biophys J. 1970 May;10(5):365–379. [PMC free article] [PubMed]
  • Maroudas A. Biophysical chemistry of cartilaginous tissues with special reference to solute and fluid transport. Biorheology. 1975 Jun;12(3-4):233–248. [PubMed]
  • Maroudas AI. Balance between swelling pressure and collagen tension in normal and degenerate cartilage. Nature. 1976 Apr 29;260(5554):808–809. [PubMed]
  • Maroudas A, Thomas H. A simple physicochemical micromethod for determining fixed anionic groups in connective tissue. Biochim Biophys Acta. 1970 Jul 21;215(1):214–216. [PubMed]
  • Maroudas A, Muir H, Wingham J. The correlation of fixed negative charge with glycosaminoglycan content of human articular cartilage. Biochim Biophys Acta. 1969 May 6;177(3):492–500. [PubMed]
  • Maroudas A, Evans H, Almeida L. Cartilage of the hip joint. Topographical variation of glycosaminoglycan content in normal and fibrillated tissue. Ann Rheum Dis. 1973 Jan;32(1):1–9. [PMC free article] [PubMed]
  • MATTHEWS BF. Composition of articular cartilage in osteoarthritis; changes in collagen/chondroitin-sulphate ratio. Br Med J. 1953 Sep 19;2(4837):660–661. [PMC free article] [PubMed]
  • STEGEMANN H. Mikrobestimmung von Hydroxyprolin mit Chloramin-T und p-Dimethylaminobenzaldehyd. Hoppe Seylers Z Physiol Chem. 1958;311(1-3):41–45. [PubMed]
  • Steven FS, Thomas H. Preparation of insoluble collagen from human cartilage. Biochem J. 1973 Sep;135(1):245–247. [PMC free article] [PubMed]
  • Stockwell RA, Scott JE. Distribution of acid glycosaminoglycans in human articular cartilage. Nature. 1967 Sep 23;215(5108):1376–1378. [PubMed]
  • von Berlepsch K. Two automated procedures for determination of uronic acid in acid glycosaminoglycans. Anal Biochem. 1969 Mar;27(3):424–432. [PubMed]

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