• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of annrheumdAnnals of the Rheumatic DiseasesCurrent TOCInstructions for authors
Ann Rheum Dis. Mar 1992; 51(3): 307–312.
PMCID: PMC1004649

Leucocyte integrin and CR1 expression on peripheral blood leucocytes of patients with rheumatoid arthritis.

Abstract

Expression of the leucocyte integrins (CD11a, b, c/CD18) and of CD35 (CR1) on leucocytes from the peripheral blood of patients with rheumatoid arthritis (RA) (n = 14) and control subjects (n = 12) was measured by flow cytometry using a rapid fixation and leucocyte preparation procedure. The mean (SE) percentages of lymphocytes expressing CD11a (RA 93.4 (1.7)%; controls 97.2 (1.8)%) and CD18 (RA 91.3 (2.3)%; controls 97.0 (2.6)%) were lower and the percentage of monocytes expressing CD11b (RA 86.9 (11.4)%; controls 78.4 (11.9)%) and CR1 (RA 62.6 (15.5)%; controls 36.6 (17.6)%) were higher in patients with RA than in controls. In addition, the mean fluorescence intensity of CD18 (RA 22.1 (2.3); controls 30.7 (2.5)) on lymphocytes was decreased and that of CD11b (RA 4.5 (0.8); controls 2.9 (0.9)) and CR1 (RA 2.4 (0.4); controls 1.5 (0.5)) on monocytes was increased in patients with RA compared with controls. The functional importance (if any) of the altered expression of the antigens on lymphocytes is not yet known. Altered expression on monocytes is consistent with activation within the circulation.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Patarroyo M, Makgoba MW. Leucocyte adhesion to cells in immune and inflammatory responses. Lancet. 1989 Nov 11;2(8672):1139–1142. [PubMed]
  • Hynes RO. Integrins: a family of cell surface receptors. Cell. 1987 Feb 27;48(4):549–554. [PubMed]
  • Kornblihtt AR, Gutman A. Molecular biology of the extracellular matrix proteins. Biol Rev Camb Philos Soc. 1988 Nov;63(4):465–507. [PubMed]
  • Hogg N. The leukocyte integrins. Immunol Today. 1989 Apr;10(4):111–114. [PubMed]
  • Arnaout MA. Structure and function of the leukocyte adhesion molecules CD11/CD18. Blood. 1990 Mar 1;75(5):1037–1050. [PubMed]
  • Miller LJ, Bainton DF, Borregaard N, Springer TA. Stimulated mobilization of monocyte Mac-1 and p150,95 adhesion proteins from an intracellular vesicular compartment to the cell surface. J Clin Invest. 1987 Aug;80(2):535–544. [PMC free article] [PubMed]
  • Pichyangkul S, Schick D, Schober W, Dixon G, Khan A. Increased expression of adhesive proteins on leukocytes by TNF alpha. Exp Hematol. 1988 Aug;16(7):588–593. [PubMed]
  • Freyer DR, Morganroth ML, Rogers CE, Arnaout MA, Todd RF., 3rd Modulation of surface CD11/CD18 glycoproteins (Mo1, LFA-1, p150,95) by human mononuclear phagocytes. Clin Immunol Immunopathol. 1988 Feb;46(2):272–283. [PubMed]
  • Harlan JM, Killen PD, Senecal FM, Schwartz BR, Yee EK, Taylor RF, Beatty PG, Price TH, Ochs HD. The role of neutrophil membrane glycoprotein GP-150 in neutrophil adherence to endothelium in vitro. Blood. 1985 Jul;66(1):167–178. [PubMed]
  • Anderson DC, Miller LJ, Schmalstieg FC, Rothlein R, Springer TA. Contributions of the Mac-1 glycoprotein family to adherence-dependent granulocyte functions: structure-function assessments employing subunit-specific monoclonal antibodies. J Immunol. 1986 Jul 1;137(1):15–27. [PubMed]
  • Philips MR, Buyon JP, Winchester R, Weissmann G, Abramson SB. Up-regulation of the iC3b receptor (CR3) is neither necessary nor sufficient to promote neutrophil aggregation. J Clin Invest. 1988 Aug;82(2):495–501. [PMC free article] [PubMed]
  • Vedder NB, Harlan JM. Increased surface expression of CD11b/CD18 (Mac-1) is not required for stimulated neutrophil adherence to cultured endothelium. J Clin Invest. 1988 Mar;81(3):676–682. [PMC free article] [PubMed]
  • Zimmerman GA, McIntyre TM. Neutrophil adherence to human endothelium in vitro occurs by CDw18 (Mo1, MAC-1/LFA-1/GP 150,95) glycoprotein-dependent and -independent mechanisms. J Clin Invest. 1988 Feb;81(2):531–537. [PMC free article] [PubMed]
  • Schleiffenbaum B, Moser R, Patarroyo M, Fehr J. The cell surface glycoprotein Mac-1 (CD11b/CD18) mediates neutrophil adhesion and modulates degranulation independently of its quantitative cell surface expression. J Immunol. 1989 May 15;142(10):3537–3545. [PubMed]
  • Schwartz BR, Harlan JM. Neutrophil membrane sulfhydryl groups are involved in stimulated neutrophil adherence to endothelium. J Leukoc Biol. 1989 Mar;45(3):177–182. [PubMed]
  • Tonnesen MG, Anderson DC, Springer TA, Knedler A, Avdi N, Henson PM. Adherence of neutrophils to cultured human microvascular endothelial cells. Stimulation by chemotactic peptides and lipid mediators and dependence upon the Mac-1, LFA-1, p150,95 glycoprotein family. J Clin Invest. 1989 Feb;83(2):637–646. [PMC free article] [PubMed]
  • Cush JJ, Lipsky PE. Phenotypic analysis of synovial tissue and peripheral blood lymphocytes isolated from patients with rheumatoid arthritis. Arthritis Rheum. 1988 Oct;31(10):1230–1238. [PubMed]
  • Emery P, Lopez AF, Burns GF, Vadas MA. Synovial fluid neutrophils of patients with rheumatoid arthritis have membrane antigen changes that reflect activation. Ann Rheum Dis. 1988 Jan;47(1):34–39. [PMC free article] [PubMed]
  • Fearon DT, Collins LA. Increased expression of C3b receptors on polymorphonuclear leukocytes induced by chemotactic factors and by purification procedures. J Immunol. 1983 Jan;130(1):370–375. [PubMed]
  • Banga JP, LeRoy F, Suleyman S, Kasp E, Brown E, Dumonde D. Analysis of antigenic determinants of retinal S-antigen with monoclonal antibodies. Invest Ophthalmol Vis Sci. 1988 Jan;29(1):12–21. [PubMed]
  • McCarthy DA, Bernhagen J, Liu YC, Perry JD. A rapid preparation technique for leucocytes. J Microsc. 1990 Apr;158(Pt 1):63–72. [PubMed]
  • Pizzolo G, Sloane J, Beverley P, Thomas JA, Bradstock KF, Mattingly S, Janossy G. Differential diagnosis of malignant lymphoma and nonlymphoid tumors using monoclonal anti-leucocyte antibody. Cancer. 1980 Dec 15;46(12):2640–2647. [PubMed]
  • Hogg N, MacDonald S, Slusarenko M, Beverley PC. Monoclonal antibodies specific for human monocytes, granulocytes and endothelium. Immunology. 1984 Dec;53(4):753–767. [PMC free article] [PubMed]
  • Beverley PC, Callard RE. Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody. Eur J Immunol. 1981 Apr;11(4):329–334. [PubMed]
  • Hildreth JE, Gotch FM, Hildreth PD, McMichael AJ. A human lymphocyte-associated antigen involved in cell-mediated lympholysis. Eur J Immunol. 1983 Mar;13(3):202–208. [PubMed]
  • Hogg N, Takacs L, Palmer DG, Selvendran Y, Allen C. The p150,95 molecule is a marker of human mononuclear phagocytes: comparison with expression of class II molecules. Eur J Immunol. 1986 Mar;16(3):240–248. [PubMed]
  • Malhotra V, Hogg N, Sim RB. Ligand binding by the p150,95 antigen of U937 monocytic cells: properties in common with complement receptor type 3 (CR3). Eur J Immunol. 1986 Sep;16(9):1117–1123. [PubMed]
  • O'Shea JJ, Brown EJ, Seligmann BE, Metcalf JA, Frank MM, Gallin JI. Evidence for distinct intracellular pools of receptors for C3b and C3bi in human neutrophils. J Immunol. 1985 Apr;134(4):2580–2587. [PubMed]
  • Nelson RD, Hasslen SR, Ahrenholz DH, Haus E, Solem LD. Influence of minor thermal injury on expression of complement receptor CR3 on human neutrophils. Am J Pathol. 1986 Dec;125(3):563–570. [PMC free article] [PubMed]
  • Anderson DC, Freeman KL, Heerdt B, Hughes BJ, Jack RM, Smith CW. Abnormal stimulated adherence of neonatal granulocytes: impaired induction of surface Mac-1 by chemotactic factors or secretagogues. Blood. 1987 Sep;70(3):740–750. [PubMed]
  • Van Epps DE, Bender JG, Simpson SJ, Chenoweth DE. Relationship of chemotactic receptors for formyl peptide and C5a to CR1, CR3, and Fc receptors on human neutrophils. J Leukoc Biol. 1990 Jun;47(6):519–527. [PubMed]
  • Setiadi H, Wautier JL, Courillon-Mallet A, Passa P, Caen J. Increased adhesion to fibronectin and Mo-1 expression by diabetic monocytes. J Immunol. 1987 May 15;138(10):3230–3234. [PubMed]
  • Heurkens AH, Breedveld FC, Keur CV, Brand R, Daha MR. Degradation of aggregates of activated C3 (C3b) by monocytes of patients with rheumatoid arthritis is related to vasculitis. Clin Exp Immunol. 1990 May;80(2):177–180. [PMC free article] [PubMed]
  • Moore FD, Jr, Davis C, Rodrick M, Mannick JA, Fearon DT. Neutrophil activation in thermal injury as assessed by increased expression of complement receptors. N Engl J Med. 1986 Apr 10;314(15):948–953. [PubMed]
  • Babcock GF, Alexander JW, Warden GD. Flow cytometric analysis of neutrophil subsets in thermally injured patients developing infection. Clin Immunol Immunopathol. 1990 Jan;54(1):117–125. [PubMed]
  • Buyon JP, Shadick N, Berkman R, Hopkins P, Dalton J, Weissmann G, Winchester R, Abramson SB. Surface expression of Gp 165/95, the complement receptor CR3, as a marker of disease activity in systemic Lupus erythematosus. Clin Immunol Immunopathol. 1988 Jan;46(1):141–149. [PubMed]
  • McCarthy DA, Bernhagen J, Taylor MJ, Hamblin AS, James I, Thompson PW, Perry JD. Morphological evidence that activated polymorphs circulate in the peripheral blood of patients with rheumatoid arthritis. Ann Rheum Dis. 1992 Jan;51(1):13–18. [PMC free article] [PubMed]

Articles from Annals of the Rheumatic Diseases are provided here courtesy of BMJ Group

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • MedGen
    MedGen
    Related information in MedGen
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links