PMC

(a) Sun-protected (upper buttocks) or sun-exposed (dorsal forearm) on geriatric (>65 years old) volunteers were treated with dermabrasion (DA) or fractionated laser resurfacing (FLR). Images shown were obtained immediately following treatment. (b) Relative COL1A expression was assayed by QRT-PCR (standardized by actin expression)from biopsies obtained from sun-protected skin from young adult (20–28 years old) volunteers, sun-protected skin from geriatric volunteers, healed (3 months) sun-protected skin from geriatric patients treated with DA, healed (3 months) sun-protected skin from geriatric patients treated with FLR, and healed (3 months) sun-exposed skin from geriatric patients treated with FLR. Error bars indicate SEM; asterisks indicate significant difference from geriatric control values (p<0.05, student t-test). Young adult (n=4); geriatric control (n=23); geriatric DA (n=6); geriatric FLR sun-protected (n=6); geriatric FLR sun-exposed (n=7). NOTE: Young adult and geriatric DA data are presented in this figure for comparison; similar data have been previously reported in another format [; ].

(a) IGF-1 gene expression was measured by QRT-PCR analysis of biopsies from volunteers described in and standardized to actin expression. Error bars indicate SEM; asterisk denotes statistical significance of Geriatric Control values from all other cohorts (p<0.02; individual paired t-test). In addition, ANOVA analysis detected no significant difference between Young Adult, Geriatric DA, and Geriatric FLA cohorts. Young adult (n=4); geriatric control (n=27); geriatric DA (n=6); geriatric FLR sun-protected (n=8); geriatric FLR sun-exposed (n=8). (b) The skin of the patient cohorts described in were assayed for their response to UVB irradiation by quantifying the level of basal layer keratinocytes in the biopsies that had detectable UVB-induced DNA damage (TD+) and cellular markers of proliferation (Ki67+) at 24 hours following an exposure to UVB. Error bars indicate SEM; asterisk denotes statistical significance of Geriatric Control values from all other cohorts (p<0.001; individual paired t-test). In addition, ANOVA analysis detected no significant difference between Young Adult, Geriatric DA, and Geriatric FLA cohorts. Young adult (n=6); geriatric control (n=27); geriatric DA (n=7); geriatric FLR sun-protected (n=7); geriatric FLR sun-exposed (n=8). NOTE: Young adult and geriatric DA data are presented in this figure for comparison; similar data have been previously reported in another format [; ].

Mortality trends among geriatric trauma patients over the 10-year time period. Significant decrease was found among total geriatric patients and severe geriatric trauma patients, however, mortality in geriatric less-severe trauma patients did not differ. GL, geriatric, less-severe trauma patient group; GS, geriatric, severe trauma patient group; GT, geriatric, total trauma patient group.

(a) Biopsies from untreated control geriatric skin and geriatric skin treated with fractionated laser resurfacing (following 3 months of healing) were stained with antibodies to 53BP1 and DAPI. Senescent fibroblasts in the dermis are indentified by their persistent expression of 53BP1. Bar = 50 μm. (b) The percentage of senescent fibroblasts in the papillary dermis of the indicated skin samples were determined by quantifying the number of 53BP1(+) cells in relation to the total number of DAPI(+) fibroblasts. Image analysis was performed as described in the Materials and Methods using Nikon Elements software. Error bars indicate SEM; asterisks indicate significant difference from geriatric control values (p<0.006, student t-test). Young adult (n=3); geriatric control (n=22); geriatric DA (n=6); geriatric FLR sun-protected (n=6); geriatric FLR sun-exposed (n=7).