Display Settings:

Items per page

Results: 8

1.
Figure 7

Figure 7. From: Oxidative stress causes enhanced endothelial cell injury in human heme oxygenase-1 deficiency.

Structural organization of HO-1 gene and construction of PCR primers for mutational analysis.

Akihiro Yachie, et al. J Clin Invest. 1999 January 1;103(1):129-135.
2.
Figure 3

Figure 3. From: Oxidative stress causes enhanced endothelial cell injury in human heme oxygenase-1 deficiency.

Iron staining of the kidney and liver. Paraffin-embedded specimens of the kidney and liver were stained for iron. (a) Kidney. ×200. (b) Liver. ×200. Asterisk indicates a glomerulus.

Akihiro Yachie, et al. J Clin Invest. 1999 January 1;103(1):129-135.
3.
Figure 4

Figure 4. From: Oxidative stress causes enhanced endothelial cell injury in human heme oxygenase-1 deficiency.

Immunohistochemical staining of HO-1 in the liver. Paraffin-embedded specimen of the liver was stained with anti–HO-1 antiserum. (a) Control. (b) Patient. Arrows indicate Kupffer cells. HO-1, heme oxygenase-1.

Akihiro Yachie, et al. J Clin Invest. 1999 January 1;103(1):129-135.
4.
Figure 1

Figure 1. From: Oxidative stress causes enhanced endothelial cell injury in human heme oxygenase-1 deficiency.

Plasma and peripheral blood smears. (a) Centrifuged peripheral venous blood from control (left) and patient (right). (b) Peripheral blood smear was stained by May-Grünwald and Giemsa. Arrows indicate fragmented erythrocytes.

Akihiro Yachie, et al. J Clin Invest. 1999 January 1;103(1):129-135.
5.
Figure 2

Figure 2. From: Oxidative stress causes enhanced endothelial cell injury in human heme oxygenase-1 deficiency.

Pathology of renal glomerular loop. (a) Hematoxylin and eosin staining of the renal glomeruli. (b) Electron microscopy of glomerular capillary of the patient. Arrow indicates the detached endothelium and asterisk indicates subendothelial deposit. (c) Electron microscopy of control glomerular capillary.

Akihiro Yachie, et al. J Clin Invest. 1999 January 1;103(1):129-135.
6.
Figure 8

Figure 8. From: Oxidative stress causes enhanced endothelial cell injury in human heme oxygenase-1 deficiency.

Mutational analysis of HO-1 gene. (a) Reverse transcriptase-PCR for HO-1 mRNA. M denotes molecular weight marker. Lane 1, control; lane 2, patient; lane 3, father; and lane 4, mother. (b) Sequence analysis of paternal allele and normal control allele. (c) Sequence analysis of maternal allele and normal control allele. (d) Mutation- specific PCR of genomic DNA. Data from control (lane 1), patient (lane 2), father (lane 3), and mother (lane 4) are shown. (e) Control PCR.

Akihiro Yachie, et al. J Clin Invest. 1999 January 1;103(1):129-135.
7.
Figure 6

Figure 6. From: Oxidative stress causes enhanced endothelial cell injury in human heme oxygenase-1 deficiency.

Cytotoxicity assay. LCLs from patient (closed circles) and control (open circles) were cultured for 24 h with different concentrations of hemin. Dead or apoptotic cells were identified by lowered forward light scatter and high annexin-V binding using a flow cytometer. Percent survival was determined compared with cells cultured with medium. Each datum represents the mean ± SD of five independent experiments. *P < 0.001.

Akihiro Yachie, et al. J Clin Invest. 1999 January 1;103(1):129-135.
8.
Figure 5

Figure 5. From: Oxidative stress causes enhanced endothelial cell injury in human heme oxygenase-1 deficiency.

Immunoblotting of HO-1 induced in cadmium-stimulated cells. (a) PBMC from patient (lanes 1 and 2) and control 1 (lanes 3 and 4). Control 2 (lanes 5 and 6), and control 3 (lanes 7 and 8) were cultured alone (lanes 1, 3, 5, and 7) or with cadmium (lanes 2, 4, 6, and 8). Lysate samples were prepared as shown in Methods. Blotted membrane was reacted simultaneously with anti–HO-1 antiserum and anti–HO-2 antiserum, followed by the reaction with HRP-conjugated anti–rabbit IgG. (b) LCLs from patient, parents, and control were cultured with cadmium and treated similarly. Lane 1, control; lane 2, patient; lane 3, father; and lane 4, mother. HRP, horseradish peroxidase; LCL, lymphoblastoid cell line, PBMC, peripheral blood mononuclear cells.

Akihiro Yachie, et al. J Clin Invest. 1999 January 1;103(1):129-135.

Display Settings:

Items per page

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk