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1.
Figure 7

Figure 7. From: The TEL/ETV6 gene is required specifically for hematopoiesis in the bone marrow.

 The requirement for TEL in the development of the hematopoietic system. Arrows depict the colonization routes of HSC/multipotent progenitors.

Li Chun Wang, et al. Genes Dev. 1998 August 1;12(15):2392-2402.
2.
Figure 3

Figure 3. From: The TEL/ETV6 gene is required specifically for hematopoiesis in the bone marrow.

TEL−/− ES cell progneitors do not contribute to mature erythroid lineages in chimeras. Red blood cells from young and adult chimeric mice were subject to hemoglobin analysis. [H (Hbbs)] Specific for host (C57BL/6) blastocyst cells; [ES (Hbbd)] specific for ES (129/Sv) cells. (*) Lack of adult β-hemoglobin contribution from TEL−/− ES-cell-derived chimeras.

Li Chun Wang, et al. Genes Dev. 1998 August 1;12(15):2392-2402.
3.
Figure 5

Figure 5. From: The TEL/ETV6 gene is required specifically for hematopoiesis in the bone marrow.

TEL−/− ES cells contribute to thymic T cells prior to birth. TEL+/− and TEL−/− ES cells were injected into RAG-2−/− blastocysts, and flow cytometry of thymocytes was performed at E18. The majority of the thymocytes at E18 are CD4+CD8+. Representative FACS analysis from 2 TEL+/− and 2 TEL−/− chimeras are shown. The control represents an embryo that lacked detectable ES cell contribution.

Li Chun Wang, et al. Genes Dev. 1998 August 1;12(15):2392-2402.
4.

Figure 4. From: The TEL/ETV6 gene is required specifically for hematopoiesis in the bone marrow.

TEL−/− ES-derived cells do not reconstitute efficiently lymphoid lineages in RAG-2−/− chimeras. Flow cytometry was performed on hematopoietic compartments of the TEL/RAG-2 chimeras. Anti-B220 antibody was used as B-cell marker for bone marrow and spleen cells. Anti-Ly 9.1 antibody distinguishes ES-derived from RAG-2−/− blastocyst-derived lymphocytes (the latter are Ly9.1). (A) Anti-CD4 and CD8 antibodies were used as T-cell markers for thymus and lymph-node cells. (B) The total numbers of lymph-node T cells are indicated. The dotted rectangles highlight the differences in the frequency of reconstituting lymphocytes between the TEL+/− and TEL−/− ES-cell-derived chimeras.

Li Chun Wang, et al. Genes Dev. 1998 August 1;12(15):2392-2402.
5.
Figure 1

Figure 1. From: The TEL/ETV6 gene is required specifically for hematopoiesis in the bone marrow.

TEL mRNAs are expressed in hematopoietic tissues and cell lines. (A,B) In situ hybridization of TEL transcripts was performed on E14.5 paraffin-embedded embryos as described previously (Wang et al. 1997). (Th) Thymus; (Ht) heart; (Lv) liver; (Ln) lung. (C) Northern blot analysis of poly(A)+ RNA isolated from the indicated murine cell lines. Hybridization with TEL cDNA probes is shown above. Hybridization with GAPDH cDNA as a control is shown below. (Ma) Mast cells; (MK) megakaryocytes; (T) T lymphoid.

Li Chun Wang, et al. Genes Dev. 1998 August 1;12(15):2392-2402.
6.
Figure 6

Figure 6. From: The TEL/ETV6 gene is required specifically for hematopoiesis in the bone marrow.

TEL−/− ES cells fail to contribute to adult hematopoietic tissues in chimeras. Southern blots were performed using DNAs of various adult tissues from TEL+/− or TEL−/− ES-cell-derived chimeras in the RAG-2 or wild-type (WT) backgrounds (A) and hematopoietic cells derived from yolk sacs or fetal livers (B). (wt) Wild-type alleles; (mt) mutant alleles; (Bm) bone marrow; (B) brain; (H) heart; (K) kidney; (L) liver; (M) muscle; (I) intestine; (Sp) spleen; (T) tail; (Th) thymus; (*) hematopoietic tissues (BM, Sp, and Th).

Li Chun Wang, et al. Genes Dev. 1998 August 1;12(15):2392-2402.
7.
Figure 2

Figure 2. From: The TEL/ETV6 gene is required specifically for hematopoiesis in the bone marrow.

 Assay of ES-cell-derived G418-resistant hematopoietic colonies in TEL/wild-type chimeras. (A) G418 selection strategy to distinguish hematopoietic colonies derived from wild-type (WT) or ES origins in the TEL/wild-type chimeras (see Results). Following injection of TEL+/− and TEL−/− ES cells into wild-type C57BL/6 blastocysts, single-cell suspensions are prepared from hematopoietic organs at different stages [yolk sac (E10.5), fetal liver (E14.5–E16.5), perinatal, adult]. Cells are plated in methylcellulose cultures supplemented with various growth factors, either in the presense or in the absence of G418. The numbers of colonies representing each blood-cell lineage are numerated and colonies are prepared for histological examination. (neo) Neomycin; (hyg) hygromycin; (G418S) G418 sensitive; (G418r) G418 resistant. (B) Macrophage/granulocytic colonies grown in the absence (−) and in the presense (+) of G418 (left panel, 1 mg/ml; right panel, 1.5 mg/ml G418, respectively) were collected from two representative (** in Table 1) TEL/wild-type yolk-sac progenitor assays, DNAs were extracted and subjected to Southern blot analysis. (wt) Wild-type allele; (mt) mutant allele. Note that only targeted alleles (ES-cell-derived) were detected after selection in the presence of G418.

Li Chun Wang, et al. Genes Dev. 1998 August 1;12(15):2392-2402.

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