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Results: 4

1.
Figure 4

Figure 4. From: Unexpected homology between inducible cell wall protein QID74 of filamentous fungi and BR3 salivary protein of the insect Chironomus.

Alignment between Qid74 (704 residues) and BR3 (1,700 residues). The alignment was carried out using the program sim, which is available in the Molecular Biology Server Expasy.

Manuel Rey, et al. Proc Natl Acad Sci U S A. 1998 May 26;95(11):6212-6216.
2.
Figure 1

Figure 1. From: Unexpected homology between inducible cell wall protein QID74 of filamentous fungi and BR3 salivary protein of the insect Chironomus.

Alignment of the deduced Qid74 amino acid sequence of the 14 repetitions that are 59 aa in length. The sequence of the repetitive regions have been aligned to achieve maximal homology by using the pileup and bestfit programs of the Wisconsin Gene Computer Group package.

Manuel Rey, et al. Proc Natl Acad Sci U S A. 1998 May 26;95(11):6212-6216.
3.
Figure 2

Figure 2. From: Unexpected homology between inducible cell wall protein QID74 of filamentous fungi and BR3 salivary protein of the insect Chironomus.

Relationship among the sequence of repetitive regions of the qid74 gene. The lower middle section of matrix lists the average similarity for all pairwise comparisons between the individual repeats at amino acid levels. The upper middle section shows the average of mutations in the third position.

Manuel Rey, et al. Proc Natl Acad Sci U S A. 1998 May 26;95(11):6212-6216.
4.
Figure 3

Figure 3. From: Unexpected homology between inducible cell wall protein QID74 of filamentous fungi and BR3 salivary protein of the insect Chironomus.

Detection of the qid74 gene in different species. (A) Southern blot analysis of genomic DNA, digested with BamHI (B) and XmaI (X). (B) PCR amplification of the ORF of qid74, using the 5′ (Q74up) and 3′ (Q74lo2) ends as sense and antisense primers (see Materials and Methods). (C) Variation of tandemly repeating sequences analysis. A single-copy sequence oligonucleotide (Q74up) was used as sense primer and, as antisense, a oligonucleotide (Q74lo1) was used that would hybridize with different melting temperatures to a number of nonidentical repeats. In this condition, 10 bands, which include from the ORF of repeat C to the ORF of repeat L, were expected in T. harzianum 2413. Hypocrea jecorina showed the same pattern of T. reesei (data not shown). Lanes: M, molecular weight marker; 1, T. harzianum 2413; 2, T. viride; 3, T. virens; 4, T. harzianum IMI; 5, T. longibrachiatum; 6, T. koningii; 7, T. reesei; 8, Hypocrea jecorina; T, bands expected in T. harzianum 2413; EB, expected bands in T. harzianum 2413.

Manuel Rey, et al. Proc Natl Acad Sci U S A. 1998 May 26;95(11):6212-6216.

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