We are sorry, but NCBI web applications do not support your browser and may not function properly. More information

Results: 5

1.
Figure 4

Figure 4. From: MMS2, encoding a ubiquitin-conjugating-enzyme-like protein, is a member of the yeast error-free postreplication repair pathway.

mms2 is hypostatic to rad6Δ1–9 with respect to both UV (A) and MMS (B) sensitivity. □, DBY747 (wild type); ▪, SBU (mms2); ○, WXY9376/pSCW-rad6Δ1–9 (rad6Δ1–9); •, SBU6L/pSCW-rad6Δ1–9 (mms2 rad6Δ1–9). The results are an average of two independent experiments.

Stacey Broomfield, et al. Proc Natl Acad Sci U S A. 1998 May 12;95(10):5678-5683.
2.
Figure 3

Figure 3. From: MMS2, encoding a ubiquitin-conjugating-enzyme-like protein, is a member of the yeast error-free postreplication repair pathway.

mms2 is synergistic to rev3 with respect to both UV (A) and MMS (B) sensitivity. □, DBY747 (wild type); ▪, SBU (mms2); ○, WXY9382 (rev3Δ); •, SBUr3L (rev3Δmms2). The results are an average of two independent experiments.

Stacey Broomfield, et al. Proc Natl Acad Sci U S A. 1998 May 12;95(10):5678-5683.
3.
Figure 2

Figure 2. From: MMS2, encoding a ubiquitin-conjugating-enzyme-like protein, is a member of the yeast error-free postreplication repair pathway.

Epistatic analyses of mms2 with radiation repair pathway mutations with respect to UV sensitivity. (A–D) □, DBY747 (wt); ▪, SBU (mms2). (A) mms2 and rad6. ○, WXY9376 (rad6Δ); •, SBU6L (rad6Δ mms2). (B) mms2 and rad18Δ. ○, WXY9326 (rad18Δ); •, SBU18T (rad18Δ mms2). (C) mms2 and rad4Δ. ○, WXY9394 (rad4Δ); •, SBL4U (rad4Δ mms2). (D) mms2 and rad50Δ. ○, WXY9579 (rad50Δ); •, SBU50h (rad50Δ mms2). The results are an average of two to four independent experiments.

Stacey Broomfield, et al. Proc Natl Acad Sci U S A. 1998 May 12;95(10):5678-5683.
4.
Figure 5

Figure 5. From: MMS2, encoding a ubiquitin-conjugating-enzyme-like protein, is a member of the yeast error-free postreplication repair pathway.

Schematic diagram of possible Rad6-mediated metabolic pathways. Rad6 forms distinct complexes with either Ubr1 or Rad18; the Rad6–Rad18 complex is proposed to be responsible for DNA repair (19–21). The DNA repair component consists of an N-terminal-dependent error-free PRR pathway and an N-terminal-independent error-prone mutagenesis pathway. Mms2, PCNA, Polδ, and Rad30 are proposed to participate in the error-free PRR, whereas Rev1, -3, and -7 are responsible for mutagenesis.

Stacey Broomfield, et al. Proc Natl Acad Sci U S A. 1998 May 12;95(10):5678-5683.
5.
Figure 1

Figure 1. From: MMS2, encoding a ubiquitin-conjugating-enzyme-like protein, is a member of the yeast error-free postreplication repair pathway.

Physical characterization of the S. cerevisiae MMS2 gene. (A) Mapping and disruption of the MMS2 gene. A subclone containing the 3-kb XbaI–HindIII fragment enables (+) the mms2-1 mutant to grow on YPD plates containing 0.4% MMS. Further deletions to the NcoI site from either end abolished (−) the MMS2 function. Either a URA3 or a LEU2 fragment was inserted at the NcoI site to construct the mms2URA3 and mms2LEU2 disruption cassettes. (B) Killing of DBY747 (wt), WX17–4a (mms2-1), and SBU (mms2) in a liquid culture containing 0.3% MMS. (C) The nucleotide and deduced amino acid sequences of the MMS2 gene (GenBank accession no. U66724). Exons are in boldface. Lowercase indicates intron sequences. Consensus sequences within the intron are underlined. The translation initiation site for MAD1 and the transcriptional termination site for SNR10 are indicated with an arrow for direction. A C-to-T transition found in the mms2-1 mutation at nucleotide 303 is marked. (D) Amino acid sequence alignments of Mms2 with two yeast Ubc proteins, Rad6 (Ubc2) and Ubc4. Residues shared by two or more proteins are highlighted. (E) Amino acid sequence alignment of Mms2 with Croc1. Residues in Croc1 identical to Mms2 are highlighted.

Stacey Broomfield, et al. Proc Natl Acad Sci U S A. 1998 May 12;95(10):5678-5683.

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk