Results: 4

1.
Figure 2

Figure 2. From: Targeted disruption of the mouse gene encoding steroidogenic acute regulatory protein provides insights into congenital lipoid adrenal hyperplasia.

Histology of the adrenal gland from wild-type and StAR knockout mice. Adrenal glands were isolated from wild-type and StAR knockout mice 6 days after birth. (A) Hematoxylin/eosin staining of wild-type adrenal section. (B) Hematoxylin/eosin staining of StAR knockout adrenal section. (C) Oil red O staining of section from wild-type adrenal. (D) Oil red O staining of section from StAR knockout adrenal.

Kathleen M. Caron, et al. Proc Natl Acad Sci U S A. 1997 October 14;94(21):11540-11545.
2.
Figure 3

Figure 3. From: Targeted disruption of the mouse gene encoding steroidogenic acute regulatory protein provides insights into congenital lipoid adrenal hyperplasia.

Sex-dependent differences in gonadal effects of StAR deficiency. Testes and ovaries were isolated from wild-type and StAR knockout mice 7 days after birth, and frozen sections were stained with oil red O. (A) StAR knockout testis. (B) Wild-type testis. (C) StAR knockout ovary. (D) Wild-type ovary.

Kathleen M. Caron, et al. Proc Natl Acad Sci U S A. 1997 October 14;94(21):11540-11545.
3.
Figure 4

Figure 4. From: Targeted disruption of the mouse gene encoding steroidogenic acute regulatory protein provides insights into congenital lipoid adrenal hyperplasia.

Developmental analysis of lipid deposition in the testes of StAR knockout embryos. Embryos from timed matings were collected at embryonic day 14.5, and transverse sections were analyzed by staining with oil red O. (A) Lower power magnification of section from wild-type testis. (B) Lower power magnification of section from StAR knockout testis. (C) Higher power view of section from wild-type testis. (D) Higher power view of section from StAR knockout testis. Arrows point to regions of lipid deposition. t, Testis; m, metanephros.

Kathleen M. Caron, et al. Proc Natl Acad Sci U S A. 1997 October 14;94(21):11540-11545.
4.
Figure 1

Figure 1. From: Targeted disruption of the mouse gene encoding steroidogenic acute regulatory protein provides insights into congenital lipoid adrenal hyperplasia.

Generation of StAR knockout mice by gene targeting in embryonic stem cells. (A) Strategy to disrupt the StAR locus. A diagram of the mouse StAR gene is shown. Upon homologous recombination, the targeting vector will delete part of exon 2 and all of exon 3. The targeting vector was electroporated into embryonic stem cells and clones screened by PCR analysis as described. (B) Pedigree analysis from mating of heterozygous +/− mice. Genomic DNA was isolated from pups born from a mating of StAR +/− mice, and genotypes were determined by Southern blot analysis with the probe shown in A. The smaller (4.5 kb) band results from the wild-type locus, and the larger (5.1 kb) band results from the disrupted locus. (C) Immunoblot analysis of StAR protein in StAR knockout mice. Tissue extracts from the indicated sources were prepared and 6 μg of the MA-10 mitochondrial extract or 15 μg of all other extracts were analyzed by immunoblotting with an antibody specific for StAR. Numbers on the left indicate the positions of molecular weight markers. Mature StAR protein, which has an apparent Mr of 30,000, is absent in the extract from the StAR knockout adrenals but readily detected in the extract from wild-type adrenals.

Kathleen M. Caron, et al. Proc Natl Acad Sci U S A. 1997 October 14;94(21):11540-11545.

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