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Results: 6

1.
Figure 1

Figure 1. From: The human XRCC9 gene corrects chromosomal instability and mutagen sensitivities in CHO UV40 cells.

Schematic diagram of cloning strategy. The primers used for amplifying the XRCC9 cDNA by PCR are indicated by arrows.

Nan Liu, et al. Proc Natl Acad Sci U S A. 1997 August 19;94(17):9232-9237.
2.
Figure 4

Figure 4. From: The human XRCC9 gene corrects chromosomal instability and mutagen sensitivities in CHO UV40 cells.

Differential cytotoxicity to MMC of wild-type AA8, mutant UV40, and cDNA transformant 40cXR9.31. Cells (2 × 104) were inoculated in 12-well trays and incubated with MMC for 4 days (AA8) or 7 days (UV40 and 40cXR9.31) before fixation. Trays were stained with crystal violet.

Nan Liu, et al. Proc Natl Acad Sci U S A. 1997 August 19;94(17):9232-9237.
3.
Figure 3

Figure 3. From: The human XRCC9 gene corrects chromosomal instability and mutagen sensitivities in CHO UV40 cells.

Human chromosomal localization of XRCC9. (A) FISH image of a human metaphase cell showing hybridization of the genomic probe to chromosome 9p13. (B) DAPI staining of the same metaphase showing bright staining of the centromeric region of a C-group chromosome, which is diagnostic of chromosome 9.

Nan Liu, et al. Proc Natl Acad Sci U S A. 1997 August 19;94(17):9232-9237.
4.
Figure 5

Figure 5. From: The human XRCC9 gene corrects chromosomal instability and mutagen sensitivities in CHO UV40 cells.

Northern blots of hamster and human poly(A)+ RNA. Four micrograms of poly(A)+ RNA from each cell line was loaded per lane and probed with a 1.5-kb XRCC9 cDNA fragment (A) and subsequently with GAPDH cDNA (B). The film was exposed for 16 h or 1 h for A and B, respectively. (C) Location within the cDNA of the 1.5-kb fragment used in hybridization. The ORF is shown by the bold line.

Nan Liu, et al. Proc Natl Acad Sci U S A. 1997 August 19;94(17):9232-9237.
5.
Figure 2

Figure 2. From: The human XRCC9 gene corrects chromosomal instability and mutagen sensitivities in CHO UV40 cells.

Nucleotide sequence of XRCC9 cDNA and the translated amino acid sequence. The consensus translation initiation sequence is indicated in bold. The start and stop codons and a leucine zipper region are underlined. An in-frame stop codon (taa) upstream of the ATG start codon and the polyadenylation signal (agtaaa) upstream of the polyadenylation site are boxed.

Nan Liu, et al. Proc Natl Acad Sci U S A. 1997 August 19;94(17):9232-9237.
6.
Figure 6

Figure 6. From: The human XRCC9 gene corrects chromosomal instability and mutagen sensitivities in CHO UV40 cells.

Northern blots of poly(A)+ RNA showing relative XRCC9 mRNA expression in tissues of baboon and human. Baboon RNAs were probed with XRCC9 (A) or GAPDH (B) cDNA. Lanes: 1, brain; 2, heart; 3, kidney; 4 liver; 5, lung; 6, lymph node; 7, ovary; 8, spleen; 9, testis. Human RNAs were probed with XRCC9 (C) or actin (D) cDNA. Lanes: 1, spleen; 2, thymus; 3, prostate; 4, testis; 5, ovary; 6, small intestine; 7, colon (mucosal lining); 8, peripheral blood leukocyte. XRCC9 signals were normalized to reference RNAs as shown using cpm.

Nan Liu, et al. Proc Natl Acad Sci U S A. 1997 August 19;94(17):9232-9237.

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