Display Settings:

Items per page
We are sorry, but NCBI web applications do not support your browser and may not function properly. More information

Results: 6

1.
Figure 1

Figure 1. Frequencies of p11C- and p54AS-specific CD8+ T cells and plasma viral loads during primary infection.. From: TCR Affinity Associated with Functional Differences between Dominant and Subdominant SIV Epitope-Specific CD8+ T Cells in Mamu-A*01+ Rhesus Monkeys.

A) Mean frequencies of the p11C- and p54AS-specific CD8+ T cells in peripheral blood. * significant at p≤0.05. B) Plasma SIV RNA levels in the peripheral blood.

Christa E. Osuna, et al. PLoS Pathog. 2014 April;10(4):e1004069.
2.
Figure 4

Figure 4. The dominant p11C-specific cells exhibited decreased antigen-specific expansion compared to subdominant epitope-specific cells.. From: TCR Affinity Associated with Functional Differences between Dominant and Subdominant SIV Epitope-Specific CD8+ T Cells in Mamu-A*01+ Rhesus Monkeys.

PBMCs from monkeys chronically-infected with either SIVmac251 (A) or SIVsmE660 (B) were stimulated in vitro with either p11C (red), p54E660/AS (blue), or p68A (green) peptide, harvested on days 3, 4, 5, 6, 8, 10, 12, and 14 following stimulation, and measured by flow cytometry to calculate the percent of tetramer-positive CD8+ T cells. Expansion was calculated as the fold change of the percent of each tetramer-positive population on each day, relative to day 0. Data from three SIVmac251- and three SIVsmE660-infected monkeys are shown. Measurements were conducted between weeks 40–52 for SIVmac251 and 31–44 for SIVsmE660.

Christa E. Osuna, et al. PLoS Pathog. 2014 April;10(4):e1004069.
3.
Figure 2

Figure 2. Genes differentially expressed between dominant p11C- and subdominant p54AS-specific CD8+ T cells.. From: TCR Affinity Associated with Functional Differences between Dominant and Subdominant SIV Epitope-Specific CD8+ T Cells in Mamu-A*01+ Rhesus Monkeys.

The median of the normalized expression values, measured in fluorescence units, of A) maturation, B) cytotoxicity, and C) proliferation and apoptosis genes that were found to be differentially expressed between the p11C- and p54AS-specific cells on at least one time point are shown for each timepoint evaluated during the first 70 days following SIVmac251 infection. The expression of these genes by total naïve CD8+ T cells, measured on day 0, is also shown. For those genes with more than one probe on the BeadChip, the numerical probe IDs are included in the gene name. * indicates that expression met the criteria for differential expression on that timepoint.

Christa E. Osuna, et al. PLoS Pathog. 2014 April;10(4):e1004069.
4.
Figure 5

Figure 5. Dominant p11C-specific population contained lower frequency of cytokine- and chemokine-producing cells than subdominant epitope-specific populations.. From: TCR Affinity Associated with Functional Differences between Dominant and Subdominant SIV Epitope-Specific CD8+ T Cells in Mamu-A*01+ Rhesus Monkeys.

PBMCs from monkeys chronically-infected with either SIVmac251 (n = 4) or SIVsmE660 (n = 5) were stimulated with either p11C, p54AS/E660, or p68A peptides and intracellular staining was used to assess production of the chemokine MIP-1β and cytokines IFNγ, TNFα, and IL-2. Bars represent mean ± SEM. A) Individual cytokine and chemokine production. Top, SIVmac251. Bottom, SIVsmE660. B) Polyfunctional analysis. Positivity for each cytokine/chemokine is indicated by the dots below the bar graph. Vertical bars are grouped into 4, 3, 2 or 1 function (indicated by the pink, light blue, purple, and orange horizontal bars, respectively). C) Polyfunctionality charts. Left, SIVmac251. Right, SIVsmE660. Each slice represents the percentage of tetramer-positive cells expressing between 1 and 4 functions. Data were collected between weeks 36–42 for SIVmac251 and 14–25 for SIVsmE660.

Christa E. Osuna, et al. PLoS Pathog. 2014 April;10(4):e1004069.
5.
Figure 6

Figure 6. TCR:pMHC measurements.. From: TCR Affinity Associated with Functional Differences between Dominant and Subdominant SIV Epitope-Specific CD8+ T Cells in Mamu-A*01+ Rhesus Monkeys.

DRMs were purified from total CD8+ T cells sorted from seven chronically-infected SIVsmE660-infected monkeys. The DRMs were evaluated for specific binding, measured in resonance units (RU), to pMHC monomers constructed with p11C, p54E660, and p68A epitope peptides and Mamu-A*01. Data are representative of the binding observed from all seven monkeys evaluated. A) Initial experiments to detect p11C, p54E660, and p68A monomer binding to DRMs. 100 μg/mL p11C (red), 100 μg/mL p54E660 (blue), and 150 μg/mL p68A (green) pMHC monomer binding are overlaid from experiments conducted on separate Biacore Chips. Binding of the p68A:Mamu-A*01 monomer to the DRMs was not observed at any concentration of monomer tested (25–200 μg/mL). B) Titrations of p11C (top) and p54E660 (bottom) peptide:Mamu-A*01 monomers for calculation of binding kinetics and affinity. Overlaid sensograms of the binding of p11C and p54E660 pMHC monomers to DRMs purified from total CD8+ T cells are shown. Monomer binding was evaluated using pMHC concentrations ranging from 25 to 200 μg/mL. The black curve shows the Langmuir fitted curve that was used to calculate kinetics. C) Detection of p68A peptide:Mamu-A*01 monomer binding to DRMs. p68A-specific CD8+ T cells were collected from multiple tetramer-specific flow cytometric cell sorts and pooled for DRM purification. Titrations of p68A pMHC monomers were performed at concentrations ranging from 150 to 1000 μg/mL. Binding to DRMs from monkey ZD57 at 1000 μg/mL is shown and is representative of the four monkeys that were evaluated. All readings have been normalized by subtracting the binding of the control monomer TL8 run at the same concentrations as the experimental monomers.

Christa E. Osuna, et al. PLoS Pathog. 2014 April;10(4):e1004069.
6.
Figure 3

Figure 3. Phenotype and cytotoxic potential of dominant and subdominant epitope-specific cells during chronic SIVmac251 and SIVsmE660 infection.. From: TCR Affinity Associated with Functional Differences between Dominant and Subdominant SIV Epitope-Specific CD8+ T Cells in Mamu-A*01+ Rhesus Monkeys.

The frequency, cell surface phenotype, and ex vivo perforin and granzyme B content were evaluated for p11C-, p54AS/E660-, and p68A-specific cells, identified with Mamu-A*01 tetramers, from peripheral blood of chronically infected SIVmac251- and SIVsmE660- infected rhesus monkeys. A) Frequencies of the p11C-, p54AS/E660-, and p68A-specific cells from (top) SIVmac251- and (bottom) SIVsmE660- infected rhesus monkeys. Error bars indicate median ± interquartile range. SIVmac251 frequencies measured between weeks 37–50, except for 133-06 which died early and data presented are from week 18. SIVsmE660 frequencies are measured between weeks 19–22. B) Phenotyping of cells from SIVmac251- (top, n = 4) and SIVsmE660-infected monkeys (bottom, n = 8) based on cell surface expression of CCR7 and CD28. Positivity for CCR7 and CD28 is indicated by the + and − signs below the bar graph. Cells were categorized as central memory (CCR7+CD28+), transitional memory (CCR7CD28+), or effector memory (CCR7CD28). All tetramer-positive cells were CD95+. Phenotyping was conducted between weeks 44–78 for SIVmac251 and 33–46 for SIVsmE660. C) Measurement of percent of ex vivo perforin+granzyme B+ cells from SIVmac251- (Left, n = 5) or SIVsmE660-infected monkeys (right, n = 8). D) Per-cell expression of perforin and granzyme B, measured by geometric mean of fluorescence (GMF) of perforin and granzyme B staining on cells from SIVmac251- (left, n = 5) and SIVsmE660-infected monkeys (right, n = 8). Perforin and granzyme B measurements were conducted between weeks 63–83 for SIVmac251 and 41–49 for SIVsmE660. In parts B–D, error bars represent mean ± SEM. * and **, significant at p≤0.05 and 0.01, respectively, after Bonferroni correction. NS, not significant.

Christa E. Osuna, et al. PLoS Pathog. 2014 April;10(4):e1004069.

Display Settings:

Items per page

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk