Results: 4

1.
Figure 1

Figure 1. From: Salmonella Typhimurium TTSS-2 deficient mig-14 mutant shows attenuation in immunocompromised mice and offers protection against wild-type Salmonella Typhimurium infection.

Analysis of MT4 attenuation in comparison to MT5 in Nos2−/−, Il-10−/−, CD40L−/−and wild-type C57BL/6 mice. Streptomycin pretreated mice were infected either with MT5 or MT4 (5x107 CFU by gavage; n = 5mice). Bacterial loads in cecum content (A), mLN (B), spleen (C) and liver (D) were assessed by plating at day 4 p.i.. n.s., statistically not significant; *, statistically significant (p < 0.05, Two-way ANOVA).

Niladri Bhusan Pati, et al. BMC Microbiol. 2013;13:236-236.
2.
Figure 3

Figure 3. From: Salmonella Typhimurium TTSS-2 deficient mig-14 mutant shows attenuation in immunocompromised mice and offers protection against wild-type Salmonella Typhimurium infection.

Analysis of colonization and cecal pathology of the vaccinated mice after wild-type S. Typhimurium challenge. Mice immunized with PBS, MT5 and MT4 (n = 5) were treated with ampicillin (25 mg by gavage), challenged with wild-type SB300 (ampr, smr) and sacrificed three days later (day 3 p.c.). Disease parameters like colonization at various host-tissues (A) and cecal pathology (B) were determined. n.s., not significant; *, statistically significant (p < 0.05).

Niladri Bhusan Pati, et al. BMC Microbiol. 2013;13:236-236.
3.
Figure 4

Figure 4. From: Salmonella Typhimurium TTSS-2 deficient mig-14 mutant shows attenuation in immunocompromised mice and offers protection against wild-type Salmonella Typhimurium infection.

Validation of antibody response (serum IgG and intestinal sIgA). Serum and gut wash from mice treated with PBS and vaccinated with MT4 and MT5 were collected, diluted to a highest dilution of 1:120 (serum) and 1:9 (gut wash). The presence of Salmonella specific IgG and secretory IgA were detected by Western blots. The representative Western blot analysis of the antibody responses was done by developing the blots of overnight grown cultures of MT5, MT4, SB300 (wild-type S. Typhimurium) and M1525 (S. Enteritidis; negative control) with the serum and gut wash of the immunized mice.

Niladri Bhusan Pati, et al. BMC Microbiol. 2013;13:236-236.
4.
Figure 2

Figure 2. From: Salmonella Typhimurium TTSS-2 deficient mig-14 mutant shows attenuation in immunocompromised mice and offers protection against wild-type Salmonella Typhimurium infection.

Vaccination experiment analyzing the attenuation of MT4 at day 30 p.v. For vaccination, C57BL/6 mice were treated with PBS (n = 10; grey solid circles), MT5 (5x107CFU; n = 10; black solid circle) and MT4 (5x107 CFU; n = 10; open circle). (A) Fecal shedding as analyzed by plating. PBS-controls: below detection limit (stripped line); (B) Colonization by the vaccine strains (MT5, n = 5 and MT4, n = 5) in cecal content, mLN, spleen and liver; (C) Cecal pathology at day 30 p.v.. n.s., not significant; *, statistically significant (p < 0.05).

Niladri Bhusan Pati, et al. BMC Microbiol. 2013;13:236-236.

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk