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1.
Fig 5

Fig 5. From: Mot1 Redistributes TBP from TATA-Containing to TATA-Less Promoters.

Loss of Mot1 results in correlated changes in gene expression and TBP binding. Heat map of the change in TBP binding between the mot1-42 strain and control (wild type [WT]) strain (10′ MNase) around TSSs. The heat map is shown in descending order by the change in expression of the associated gene in the mot1-42 strain.

Gabriel E. Zentner, et al. Mol Cell Biol. 2013 December;33(24):4996-5004.
2.
Fig 1

Fig 1. From: Mot1 Redistributes TBP from TATA-Containing to TATA-Less Promoters.

Genome-wide mapping of Mot1 and TBP on native chromatin. (A) Length distributions of paired-end fragments from Mot1 and TBP IP and input samples. Distributions were normalized such that the sum of all counts was equal to 1. (B) Tracks of TBP and Mot1 2.5-min (2.5′) and 10-min (10′) MNase IP and input data along a representative portion of the yeast genome. A zoomed-in view of a single region of Mot1 and TBP enrichment is shown to the right of the tracks. chrII, chromosome II. (C) Aggregate plots of 2.5′ and 10′ MNase Mot1, TBP, and input counts/bp at all TSSs.

Gabriel E. Zentner, et al. Mol Cell Biol. 2013 December;33(24):4996-5004.
3.
Fig 4

Fig 4. From: Mot1 Redistributes TBP from TATA-Containing to TATA-Less Promoters.

Inactivation of Mot1 causes redistribution of TBP from TATA-less to TATA-containing sites. (A) Aggregate plot of TBP control and mot1-42 ORGANIC signal (10′ MNase) around TSSs. (B) Two-dimensional histogram comparing TBP occupancy levels at TBP peaks in the mot1-42 strain with and without heat shock. (C) Two examples of TBP peaks that display increased TBP occupancy upon Mot1 inactivation. (D) Two examples of TBP peaks that display decreased TBP occupancy upon Mot1 inactivation. (E) Sequence logos of the highest scoring MEME-derived motif in the top 500 TBP peaks with increased TBP (left) and the top 500 peaks with decreased TBP (right) upon Mot1 inactivation.

Gabriel E. Zentner, et al. Mol Cell Biol. 2013 December;33(24):4996-5004.
4.
Fig 6

Fig 6. From: Mot1 Redistributes TBP from TATA-Containing to TATA-Less Promoters.

A model for the relationship of Mot1 and TBP at various classes of promoters. (A) The relationship between Mot1 and TBP at TATA-containing Pol II promoters. (i) TBP binds and sharply bends TATA DNA in the context of the SAGA complex. (ii) Mot1 associates with the TBP-DNA complex and synergizes with SAGA to rapidly dissociate TBP from DNA (iii). (B) The relationship between Mot1 and TBP at TATA-less Pol II promoters. (i) TBP binds DNA in the context of the TFIID complex (for clarity, only TAF1 is shown). (ii) The interaction of TBP with TAF1 impairs recognition of TBP by Mot1, leading to continued TBP binding. (C) The relationship between Mot1 and TBP at Pol III promoters. (i) TBP binds DNA as part of TFIIIB. (ii) The interaction of TBP with an unspecified component(s) of TFIIIB impairs the Mot1-TBP interaction, leading to sustained TBP binding.

Gabriel E. Zentner, et al. Mol Cell Biol. 2013 December;33(24):4996-5004.
5.
Fig 3

Fig 3. From: Mot1 Redistributes TBP from TATA-Containing to TATA-Less Promoters.

Mot1 binds DNA upstream of TBP. (A) Aggregate plot of 2.5′ and 10′ MNase Mot1 ORGANIC signal at 438 TBP peaks containing a strong match to the TATAWAWR TATA box consensus sequence. (B) V-plots of 2.5′ and 10′ MNase log2 (Mot1 IP/input) signal at the 438 TBP peaks analyzed in panel A. The cyan line through each plot denotes the TATAWAWR midpoint. Particles presumed to give rise to the observed fragments are depicted schematically below the right panel. A lack of interaction between Mot1 and nucleosomes is demonstrated by the white/gray particles observed upstream and downstream of TATAWAWR at 150 to 160 bp.

Gabriel E. Zentner, et al. Mol Cell Biol. 2013 December;33(24):4996-5004.
6.
Fig 2

Fig 2. From: Mot1 Redistributes TBP from TATA-Containing to TATA-Less Promoters.

TBP and Mot1 cooccupy thousands of sites, and Mot1 depends on TBP for genomic association. (A) Venn diagram showing overlap of TBP ORGANIC peaks called from the 2.5 min (2.5′) and 10′ MNase data sets. (B) Two-dimensional histogram comparing enrichment of TBP at the 3,002 overlapping TBP peaks after 2.5 min (2.5′) and 10 min (10′) MNase digestion. Sites of high TBP enrichment, found in the top right corner of the histogram, are mainly tRNA promoters. The blue line represents a regression line. (C) Aggregate plot of TBP and Mot1 IP data and input data (10′ MNase) around 3,002 TBP peaks called on the 10′ MNase data set. (D) Two-dimensional histogram comparing Mot1 and TBP occupancy levels at TBP peaks. (E) Spot assay to characterize the TBP-AA/Mot1-3FLAG strain, demonstrating that epitope tagging of Mot1 does not interfere with the previously reported lethality of TBP-AA (24). (F) Aggregate plot of Mot1 IP data (10′ MNase) around 3,002 TBP peaks with or without AA-mediated depletion of TBP.

Gabriel E. Zentner, et al. Mol Cell Biol. 2013 December;33(24):4996-5004.

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