Results: 4

1.
Figure 2

Figure 2. The percentage of stage 14 egg chambers with persisting nuclei (PN) in Atg1 germline clones (GLC) is not enhanced by the expression of Diap1 in the germ cells.. From: Combined Inhibition of Autophagy and Caspases Fails to Prevent Developmental Nurse Cell Death in the Drosophila melanogaster Ovary.

The genotypes studied were Sb (sibling control, hs-FLP/ +; Atg1Δ3D FRT nos-GAL4/ TM3, Sb); w1118 control; Atg1 GLC; nos-Diap1; Atg1 GLC nos-Diap1. The error bars indicate 2 standard deviations from the mean. The percentage of PN in Atg1 GLC nos-Diap1 flies was not significantly different from that of Atg1 GLC flies (p = .85).

Jeanne S. Peterson, et al. PLoS One. 2013;8(9):e76046.
2.
Figure 3

Figure 3. Nurse cell nuclei condense normally in egg chambers containing Atg1 follicle cell clones.. From: Combined Inhibition of Autophagy and Caspases Fails to Prevent Developmental Nurse Cell Death in the Drosophila melanogaster Ovary.

(A) Anterior end of a stage 13 egg chamber stained with DAPI (blue), LysoTracker (red) and antibody to GFP (green). The condensation of nurse cell nuclei is apparent and LysoTracker staining is normal for stage 13. The dotted lines in A′′ and A′′′ indicate mutant clones. (B–F). Egg chambers with Atg1 FCCs stained with DAPI and antibody to Ref(2)P (red) and to GFP (green). (B) The staining pattern shows that Atg1 mutant clones, the non-green follicle cells, stain brightly with Ref(2)P while the normal follicle cells (green) do not stain with Ref(2)P. Scale bar = 50 µm (C) The anterior end of a stage 13 egg chamber stained with DAPI (blue), antibody to Ref(2)P (red) and antibody to GFP. Scale bar = 50 µm. (D) Same egg chamber as in C at a higher magnification. The dotted line in D′′ and D′′′ indicates a mutant clone. Scale bar = 10 µm. (E) Another stage 13 egg chamber with the same genotype and stains as (D). Scale bar = 50 µm. (F) The same egg chamber as in E, but at a higher magnification. The dotted lines in F′′ and F′′′ indicate mutant clones. Scale bars in (A,B,C and E) = 50 µm and in (D and F) = 10 µm.

Jeanne S. Peterson, et al. PLoS One. 2013;8(9):e76046.
3.
Figure 4

Figure 4. The percentage of stage 14 egg chambers with persisting nurse cell nuclei in apoptosis and autophagy double mutants is less than that of deep orange mutants.. From: Combined Inhibition of Autophagy and Caspases Fails to Prevent Developmental Nurse Cell Death in the Drosophila melanogaster Ovary.

The genotypes shown here are Atg1 GLC nos-Diap1, Atg7[d14/d77] Dcp-1,Atg7[d14/d77] nos-Diap1, and dor4/dor8; fed (n = 47) and stv (n = 173). The numbers of persisting nuclei in each egg chamber were scored as 0, 1 to 3, 4 to 6, 7 to 9, 10 to 12 or 13 to 15. For fed flies, significantly higher frequencies of PN were obtained for Atg1GLC nos-Diap1 flies (p = .002) and for dor4/dor8 hybrids (p = .002) when compared to controls. Atg7 nos-Diap1 flies had a PN frequency that was significantly lower than controls (p = .04) and Atg7 Dcp-1 flies had a PN frequency that was not significantly different from controls (p = .55) . When compared to Atg1 GLC nos-Diap1 flies, dor4/dor8 hybrids had a significantly higher frequency of egg chambers with PN (p = .002).

Jeanne S. Peterson, et al. PLoS One. 2013;8(9):e76046.
4.
Figure 1

Figure 1. Nurse cell nuclei condense and acidify normally at the end of oogenesis when apoptosis and autophagy are inhibited.. From: Combined Inhibition of Autophagy and Caspases Fails to Prevent Developmental Nurse Cell Death in the Drosophila melanogaster Ovary.

(A–C) Egg chambers were stained with DAPI to label DNA and with LysoTracker to indicate acidification. Anterior is up. (A) Wild-type stage 12 egg chamber. (B) Wild-type stage 13 egg chamber. (C) Stage 13 egg chambers from trans-heterozygotes of Atg7[d14/d77], also homozygous for Dcp-1Prev1. (D) The percentage of stage 14 egg chambers with persisting nurse cell nuclei (PN) from well-fed or starved (stv) flies having mutations affecting autophagy, apoptosis or both. The genotypes studied were Control (Cy sibling); Atg7d14/d77; Dcp-1Prev1; nos-Diap1; Atg7d14/d77 Dcp-1Prev1; Atg7d14/d77 nos-Diap1. The error bars indicate 2 standard deviations from the mean, reflecting a 95% confidence interval. Scale bar in (A–C) = 50 µm. When PN values of fed, single mutant flies were compared to controls, the mutants had a significantly greater number of PN than controls (Atg7, p = .002; Dcp-1, p = .002; nos Diap1, p = .04). The Atg7 Dcp-1fed flies had a PN value that was not significantly different from controls (p = .55) while the Atg7 nos Diap1 fed flies had a PN value that was significantly lower than controls (p = .04).

Jeanne S. Peterson, et al. PLoS One. 2013;8(9):e76046.

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