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Results: 4

1.
Figure 1

Figure 1. From: Suppression of protein kinase C theta contributes to enhanced myogenesis In vitro via IRS1 and ERK1/2 phosphorylation.

Targeted knockdown of PKCθ. A. Western blot for PKCθ in scramble and PKCθshRNA cells normalized to β-actin. ***; P<0.0001. B) Western blot for PKCθ T538 in scramble and PKCθshRNA cells normalized to β-actin. **; P=0.0064. C) PKCθ gene expression in scramble and PKCθshRNA cells. *; P=0.017. D) PKCΔ gene expression in scramble and PKCθshRNA cells. Data ± SEM. n=3 for all experiments.

Joseph S Marino, et al. BMC Cell Biol. 2013;14:39-39.
2.
Figure 4

Figure 4. From: Suppression of protein kinase C theta contributes to enhanced myogenesis In vitro via IRS1 and ERK1/2 phosphorylation.

PKCθ regulates IRS1 and ERK-mediated differentiation. A) Western blot analysis of AKT s473 and ERK T202/Y204 in scramble and B) PKCθshRNAcells treated with insulin and with or without wortmannin and U0126.C) 10x immunoflourescence images of scramble and PKCθshRNA cells treated with wortmannin and U0126 (Green; MHC and Blue; nuclei). D) Quantification of the density of MHC+ cells. *; P<0.05 and ***; P<0.0001 compared to wortmannin treated scramble cells. $; P<0.0001 compared to U0126 treated scramble cells. #; P<0.0001 compared to wortmannin treated PKCθshRNA cells. E) Number of nuclei per MHC+ cell. ***; P<0.0001 compared to wortmannin treated scramble cells. #; P<0.0001 compared to wortmannin treated PKCθshRNA cells. Data ± SEM. n=3 for all experiments.

Joseph S Marino, et al. BMC Cell Biol. 2013;14:39-39.
3.
Figure 3

Figure 3. From: Suppression of protein kinase C theta contributes to enhanced myogenesis In vitro via IRS1 and ERK1/2 phosphorylation.

Lack of PKCθ enhances protein synthesis apart from classical IRS1 signaling. A) Rates of protein synthesis determined after 4 days in differentiation media. **; P=0.0082. B) IR tyrosine phosphorylation normalized to total IR protein. **; P<0.0077. C) IRS1 tyrosine and serine phosphorylation normalized to total IRS1 protein. *; P<0.05. ***; P=0.0005. D) AKT phosphorylation normalized to total AKT protein. **; P=0.0042. E) mTOR phosphorylation normalized to total mTOR. **; P=0.0017 F) ERK1/2 phosphorylation normalized to total ERK1/2 protein. **; P=0.0023. G) ERK5 phosphorylation normalized to total ERK5 protein. Data ± SEM. n=3 for all experiments.

Joseph S Marino, et al. BMC Cell Biol. 2013;14:39-39.
4.
Figure 2

Figure 2. From: Suppression of protein kinase C theta contributes to enhanced myogenesis In vitro via IRS1 and ERK1/2 phosphorylation.

PKCθ regulates the myogenic program. A) Light microscopy images taken at 10X of scramble and PKCθshRNA cells from Day 0 through Day 4 of differentiation. On Day 4 cells were stained for MHC expression (green) and nuclei counterstained with DAPI (Blue). B) Time course of myogenin gene expression in differentiating scramble and PKCθshRNA cells. ***; P<0.0001 between scramble and PKCθshRNA cells at indicated time points. C) MHC protein expression in scramble and PKCθshRNA day 4 myotubes normalized to β-actin. ***; P<0.0002. D) Quantification of the density of MHC+ cells. **; P=0.0015. E) Number of nuclei per MHC+ cell. ***; P=0.0008. F) Time course of Focal adhesion kinase (FAK) gene expression in differentiating scramble and PKCθshRNA cells. **; P<0.01 and ***; P<0.001 between scramble and PKCθshRNA at indicated time points. G) Time course of caveolin 3 gene expression in differentiating scramble and PKCθshRNA cells. ***; P<0.001 at indicated time point. Data ± SEM. n=3 for all experiments.

Joseph S Marino, et al. BMC Cell Biol. 2013;14:39-39.

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