Display Settings:

Items per page

Results: 6

1.
Fig. 4.

Fig. 4. From: A nanoparticle formulation that selectively transfects metastatic tumors in mice.

Comparison of CPS nanoparticles with the benchmark. (A) RLuc RLUs in intrasplenic tumors after transfection with CPS or in vivo-jetPEI nanoparticles. (B) RLuc RLUs in normal lungs after transfection with CPS or in vivo-jetPEI nanoparticles. Data are means ± SD of values collected from at least two mice per group. *P < 0.05; **P < 0.01.

Jian Yang, et al. Proc Natl Acad Sci U S A. 2013 September 3;110(36):14717-14722.
2.
Fig. 5.

Fig. 5. From: A nanoparticle formulation that selectively transfects metastatic tumors in mice.

Gross visualization of in vivo transfection. Organs from animals transfected with nanoparticles bearing the β-galactosidase gene are shown. The dark-blue 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal) product accumulates in transfected regions. (A) Intrasplenic tumors transfected with CPS and adjacent normal spleen. (B) Intrasplenic tumors transfected with in vivo-JetPEI and adjacent normal spleen. (C) Normal lungs after transfection with CPS. (D) Normal lungs after transfection with in vivo-JetPEI.

Jian Yang, et al. Proc Natl Acad Sci U S A. 2013 September 3;110(36):14717-14722.
3.
Fig. 3.

Fig. 3. From: A nanoparticle formulation that selectively transfects metastatic tumors in mice.

CPS nanoparticle characterization. (A) Proposed structure of a CPS nanoparticle. HMW, high molecular weight; LMW, low molecular weight. (B) 1H NMR of PEG5-LPEI2.5 as the shell in CPS. The chemical shifts corresponding to PEG and PEI are indicated. (C) Transmission electron microscopy of CPS.

Jian Yang, et al. Proc Natl Acad Sci U S A. 2013 September 3;110(36):14717-14722.
4.
Fig. 6.

Fig. 6. From: A nanoparticle formulation that selectively transfects metastatic tumors in mice.

Transfection of metastases with CPS nanoparticles. NOG mice bearing HCT116-Luc2 (AC) or HCT116-EGFP (D) tumors were transfected with CPS containing β-galactosidase (A–C) or RFP (D) plasmids. (A) Selected view of the transfected metastases in liver. The dark-blue X-gal product accumulates in transfected regions. (B) H&E-stained section of the transfected liver containing a cluster of the transfected metastases. Dark-blue X-gal product can be seen in the transfected tumor cells. (C) Nuclear fast red-stained section of lung containing metastases. Representative metastases and transfected cells (arrowheads) are indicated. (D) Numbers of tumor cells, transfected cells, and transfected tumor cells in the livers with metastases from four individual mice as determined by confocal scanning of dissociated cells.

Jian Yang, et al. Proc Natl Acad Sci U S A. 2013 September 3;110(36):14717-14722.
5.
Fig. 1.

Fig. 1. From: A nanoparticle formulation that selectively transfects metastatic tumors in mice.

Nanoparticle core and shell characterization. (A) Zeta potentials of LPEI88-DNA nanoparticles in double-distilled water (ddH2O) at various N:P ratios. Data represent means and standard deviation (SD) of more than two independent measurements. (B) DNA gel retardation assay. Nanoparticles were formed at various N:P ratios, and electrophoresed at 100 V for 40 min in a 0.5% agarose gel. Arrows point to free DNA and LPEI-complexed DNA. (C) PEI gel electrophoresis of LPEI88-DNA nanoparticles at various N:P ratios and free LPEI88. The LPEI that is not tightly bound to the DNA migrates into the gel and is stained, while the LPEI that is bound to the DNA remains in the well.

Jian Yang, et al. Proc Natl Acad Sci U S A. 2013 September 3;110(36):14717-14722.
6.
Fig. 2.

Fig. 2. From: A nanoparticle formulation that selectively transfects metastatic tumors in mice.

CPS formulation. (A) Relative Light Units (RLUs) generated by RLuc (RLuc RLUs) in intrasplenic tumors after transfection with nanoparticles containing LPEI88 as the shell or increasing amounts of LPEI2.5 as the shell. (B) RLuc RLUs in lungs or intrasplenic tumors after treatment with CS nanoparticles or PEGylated CS nanoparticles. (C) Gel electrophoretic analysis of PEGylation of nanoparticles generated with LPEI88 in both core and shell. Lane 1, free non-PEGylated LPEI; lane 2, LPEI purified from the shell of non-PEGylated nanoparticles; lane 3, LPEI purified from the core of non-PEGylated nanoparticles; note that the larger polymer molecules in the heterodisperse LPEI preferentially interacted with DNA and were therefore found in the core; lane 4, free LPEI incubated with non-reactive PEG as a control; lane 5, PEGylated free LPEI; lane 6, LPEI purified from the shell of PEGylated nanoparticles; lane 7, LPEI purified from the core of PEGylated nanoparticles (D) RLuc RLUs in lungs and intrasplenic tumors after transfection with PEGylated CS or CPS nanoparticles. Means and standard deviations of data collected from at least two mice per group are illustrated, * P <0.05.

Jian Yang, et al. Proc Natl Acad Sci U S A. 2013 September 3;110(36):14717-14722.

Display Settings:

Items per page

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk