Results: 5

1.
Figure 5

Figure 5. Effect of slx5Δ on the sumoylation of SUMO targets.. From: Distinct SUMO Ligases Cooperate with Esc2 and Slx5 to Suppress Duplication-Mediated Genome Rearrangements.

A) Bar graph shows the relative abundance of SUMO targets between slx5Δ and wild-type cells (black bars). Results of mms21-11 versus wild-type are included for comparison (grey bars). B) Bar graph shows the relative abundance of SUMO targets between slx5Δ siz2Δ and wild-type cells (black bars). Results of siz2Δ versus wild-type are included for comparison (grey bars). C) A proposed model of the regulation of sumoylation homeostasis in cells. Mms21-specific sumoylation is positively regulated by Esc2 and negatively regulated by Slx5 to achieve a balance of sumoylation in cells, which is critical to suppress GCRs.

Claudio P. Albuquerque, et al. PLoS Genet. 2013 August;9(8):e1003670.
2.
Figure 4

Figure 4. Effect of esc2Δ on the sumoylation of SUMO targets.. From: Distinct SUMO Ligases Cooperate with Esc2 and Slx5 to Suppress Duplication-Mediated Genome Rearrangements.

A) Bar graph shows the relative abundance of SUMO targets between esc2Δ and wild-type cells (black bars). As a comparison, the results of mms21-11 versus wild-type cells are shown in grey bars. B) Quantification of the relative abundance of SUMO targets between esc2Δ and mms21-11 mutants. C) Western blot analysis of the effect of esc2Δ on the sumoylation of SMC proteins and Rpa135. Each SUMO target was purified by anti-HA antibody and probed for its sumoylation by anti-FLAG Western blot.

Claudio P. Albuquerque, et al. PLoS Genet. 2013 August;9(8):e1003670.
3.
Figure 3

Figure 3. Identification of SUMO targets that are more specific to Mms21 than Siz1 and Siz2.. From: Distinct SUMO Ligases Cooperate with Esc2 and Slx5 to Suppress Duplication-Mediated Genome Rearrangements.

A) Grey bar shows the calculated ratio between Mms21-dependent ratio and Siz1/Siz2 dependent ratio, using the data shown in Figure 2. Black bar shows a direct comparison between mms21-11 and siz1Δ siz2Δ mutants using quantitative MS. B) Effects of mm21-11 and siz1Δ siz2Δ on the sumoylation of selected SUMO targets. Each SUMO target was purified by anti-HA antibody and probed for its sumoylation using anti-FLAG Western blot.

Claudio P. Albuquerque, et al. PLoS Genet. 2013 August;9(8):e1003670.
4.
Figure 2

Figure 2. A summary of the effect of SUMO ligase-null mutations on the abundance of SUMO targets as measured by quantitative MS.. From: Distinct SUMO Ligases Cooperate with Esc2 and Slx5 to Suppress Duplication-Mediated Genome Rearrangements.

Data are shown in the same order of siz1Δ, siz2Δ, siz1Δ siz2Δ and mms21-11 mutants from left to right for each SUMO target. Log2 scale was used to calculate the relative abundance ratios. Occasionally, some SUMO targets were not identified and are indicated as not available (N/A). Additional information can be found in Tables S3, S4, S5, S6.

Claudio P. Albuquerque, et al. PLoS Genet. 2013 August;9(8):e1003670.
5.
Figure 1

Figure 1. A new quantitative proteomics technology to characterize the SUMO-proteome.. From: Distinct SUMO Ligases Cooperate with Esc2 and Slx5 to Suppress Duplication-Mediated Genome Rearrangements.

A) Method to identify and quantify sumoylated proteins using SILAC and MS. Untagged cells and HF-SUMO cells were combined for purification of sumoylated proteins. Ulp1 was used to elute sumoylated proteins for MS analysis. B) Scatter plot of the identified proteins based on two replicate experiments. The majority of the proteins show a large abundance ratio between HF-SUMO purification versus mock purification, indicating a highly purified sample. Candidate SUMO targets are identified based on at least 10-fold abundance ratio between HF-SUMO and mock sample in both replicate experiments. C) Western blot analysis of several SUMO targets confirmed them being sumoylated. Ulp1 treatment was used to treat half of the anti-HA immunoprecipitated sample.

Claudio P. Albuquerque, et al. PLoS Genet. 2013 August;9(8):e1003670.

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk