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Results: 5

1.
Figure 2

Figure 2. Amino acid alignment of human LTB4DH/PTGR1 with its closest zebrafish orthologue.. From: An Enzyme That Inactivates the Inflammatory Mediator Leukotriene B4 Restricts Mycobacterial Infection.

Identical residues are starred. Residues predicted by crystal structure to interact with NADP+ are shaded with light blue boxes13.

David M. Tobin, et al. PLoS One. 2013;8(7):e67828.
2.
Figure 4

Figure 4. a) Quantitation of bacterial burden by FPC at 3 dpi of sibling controls or ltb4dh morphants with 90±10 CFU wildtype M. marinum in vehicle (0.5% DMSO) alone or treated with 100 nM PD146176.. From: An Enzyme That Inactivates the Inflammatory Mediator Leukotriene B4 Restricts Mycobacterial Infection.

Statistical comparisons by one-way ANOVA with Tukey’s post hoc test. b) Relative TNF levels in 1 dpi larvae after injection with LTB4DH morpholino and infection with 150–200 CFU M. marinum with or without the addition of 500 nM PD146176.

David M. Tobin, et al. PLoS One. 2013;8(7):e67828.
3.
Figure 5

Figure 5. (a) LTA4H and LTB4DH mRNA expression from 14 in lymphoblastoid cell lines (LCLs) from Asian cohorts represented in the HapMap project (CHB+JPT).. From: An Enzyme That Inactivates the Inflammatory Mediator Leukotriene B4 Restricts Mycobacterial Infection.

R2 = 0.2, P<0.0001; linear regression and F test. (b) Quantitation of bacterial burden by FPC at 3 dpi of sibling controls and LTA4H-high overexpressing simultaneously or not ltb4dh with 90±10 CFU wildtype M. marinum. Statistical comparisons by one-way ANOVA with Tukey’s post hoc test.

David M. Tobin, et al. PLoS One. 2013;8(7):e67828.
4.
Figure 3

Figure 3. a) Percentage of animals with cording at 4 dpi from 34 ltb4dh morphants and 45 wildtype siblings infected with 100–150 wildtype M. marinum.. From: An Enzyme That Inactivates the Inflammatory Mediator Leukotriene B4 Restricts Mycobacterial Infection.

P = 0.003; Fisher’s exact test of contingency table. b) Percentage of animals in Fig. 1g with cording at 4 dpi among sibling wildtype, ltb4dh morphant and ltb4dh/tnf double morphant animals. **P = 0.004, *P = 0.043; Fisher’s exact test of contingency table comparing wildtype and ltb4dh/tnf double morphant each to ltb4dh morphant. c) Percentage of animals in Fig. 1h with cording at 4 dpi among sibling widtype, ltb4dh morphants treated with 10 µM U75302 and ltb4dh morphants in vehicle (0.5% DMSO). ***P<0.0001, **P = 0.01; Fisher’s exact test of contingency table comparing wildtype and U75302-treated ltb4dh morphant each to vehicle-treated ltb4dh morphant.

David M. Tobin, et al. PLoS One. 2013;8(7):e67828.
5.
Figure 1

Figure 1. (a) Schematic of LTB4 pathway, with proposed roles for the enzymes LTA4H and LTB4DH in synthesis and inactivation of LTB4, respectively highlighted in red.. From: An Enzyme That Inactivates the Inflammatory Mediator Leukotriene B4 Restricts Mycobacterial Infection.

(b) Mean ratio (±SEM) of mutant to wildtype mRNA levels in uninfected 3 dpf lta4hzm5961 animals compared to wildtype animals in four biological replicates. P = 0.007; one sample, two-tailed t test. (c) Number of cells recruited to hindbrain ventricle 6 hpi of sibling wildtype or ltb4dh morphant embryos after infection at 24 hpf with 90–100 CFU M. marinum. P = 0.005; Students’s unpaired t-test. (d) Mean(±SEM) number of bacteria per infected macrophage in 14 wildtype and 14 ltb4dh morphant siblings at 72 hpi after infection with 100–150 erp mutant bacteria. P = 0.003; Student’s unpaired t-test. Representative of two independent experiments. (e) Quantitation of bacterial burden by FPC at 5 dpi after infection of sibling controls or ltb4dh morphants with 79±21 CFU wildtype M. marinum. P = 0.002; Student’s unpaired t-test. Representative of four independent experiments. (f) Representative images of wildtype granuloma and bacterial extracellular cording in ltb4dh morphant. Scale bars, 20 µM. (g) Quantitation of bacterial burden by FPC at 3 dpi of sibling controls, ltb4dh morphants and ltb4dh/TNF double morphants with 90±10 CFU wildtype M. marinum. Statistical comparisons by one-way ANOVA with Tukey’s post hoc test. (h) Quantitation of bacterial burden by FPC at 4 dpi after infection of sibling controls or ltb4dh morphants in vehicle (0.5% DMSO) alone or treated with 10 µM U75302. (i) Relative TNF levels in wildtype siblings or ltb4dh morphant larvae 24 hpi with 150–200 CFU M. marinum with or without the addition of 10 µM U75302.

David M. Tobin, et al. PLoS One. 2013;8(7):e67828.

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