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Results: 4

1.
Figure 1

Figure 1. From: Baicalin Inhibits IL-17-Mediated Joint Inflammation in Murine Adjuvant-Induced Arthritis.

Baicalin alleviates ankle inflammation in a murine adjuvant-induced arthritis model. (a) Chemical structure of Baicalin. (b) Adjuvant-induced arthritis mice were injected intraperitoneally daily with 100 mg/kg Baicalin or phosphate-buffered saline (vehicle control) between postimmunization days 14 to 21 and photographs of the swollen ankles were acquired. (c) Ankle widths of the adjuvant-induced arthritis mice (n = 6 for each group).

Xue Yang, et al. Clin Dev Immunol. 2013;2013:268065.
2.
Figure 2

Figure 2. From: Baicalin Inhibits IL-17-Mediated Joint Inflammation in Murine Adjuvant-Induced Arthritis.

Baicalin inhibits ankle joint inflammation injury in adjuvant-induced arthritis mice. Adjuvant-induced arthritis mice were injected intraperitoneally daily with 100 mg/kg Baicalin or phosphate-buffered saline (vehicle control) for 1 week. Hematoxylin and eosin staining of control mice (a), adjuvant-induced arthritis mice with vehicle control treatment (b), and adjuvant-induced arthritis mice with Baicalin treatment (c). Further magnification of the black-bordered box showed the typical inflammatory injuries in control mice (d), adjuvant-induced arthritis mice with vehicle control treatment (e), and adjuvant-induced arthritis mice with Baicalin treatment (f). (g) Histopathological scores of the joint tissues (n = 6 for each group).

Xue Yang, et al. Clin Dev Immunol. 2013;2013:268065.
3.
Figure 4

Figure 4. From: Baicalin Inhibits IL-17-Mediated Joint Inflammation in Murine Adjuvant-Induced Arthritis.

Baicalin inhibits IL-17-mediated inflammation reactions in vitro. (a) Synoviocytes (10,000 cells/well) were plated in 12-well tissue culture plates, allowed to adhere, and were then stimulated with 50 ng/mL IL-17 with or without 20 μM Baicalin for 24 hours. Following IL-17/Baicalin stimulation, isolated splenocytes (50,000 cells/well) were added to the synoviocyte monolayers for another 24 hours. After washing twice, splenocyte adherence to synoviocytes was enumerated by microscopy at 200x magnification. Arrows indicate adhesive splenocytes (left). The number of adherent splenocytes is shown on the right. These experiments were performed three times with similar results. In mRNA expression studies, synoviocytes were cultured with 50 ng/mL IL-17 in the presence or absence 20 μM Baicalin for 24 hours, and gene expression levels of ICAM-1 (b), VCAM-1 (c), IL-6 (d), and TNF-α (e) were examined by real-time RT-PCR. These experiments were performed three times with similar results.

Xue Yang, et al. Clin Dev Immunol. 2013;2013:268065.
4.
Figure 3

Figure 3. From: Baicalin Inhibits IL-17-Mediated Joint Inflammation in Murine Adjuvant-Induced Arthritis.

Baicalin inhibits splenic Th17 cell expansion in vivo. Adjuvant-induced arthritis mice were injected intraperitoneally daily with 100 mg/kg Baicalin or phosphate-buffered saline (vehicle control) between postimmunization days 14 to 21. (a) Spleen index of control and adjuvant-induced arthritis mice with or without Baicalin treatment (n = 6 for each group). Spleen photographs are shown in the lower panels (b). Isolated splenocytes were stained and sorted by flow cytometry first for CD4+ T cells. IL-17+ T cells were analyzed in the CD4+ gate (left). The percentage of dual positive CD4+IL-17+ cells among the gated CD4+ T cell fraction in each treatment group is shown on the right (n = 6 for each group). (c) IL-17 gene expression in joint tissues was analyzed by RT-PCR. These experiments were performed three times with similar results. (d) IL-17 gene expression in joint tissues was analyzed by real-time RT-PCR (n = 6 for each group).

Xue Yang, et al. Clin Dev Immunol. 2013;2013:268065.

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