Display Settings:

Items per page

Results: 6

1.
Fig. 3

Fig. 3. From: Tie2-dependent VHL knockdown promotes airway microvascular regeneration and attenuates invasive growth of Aspergillus fumigatus.

Analysis of angiogenic cytokines and cognate receptors. a Real time RT-PCR analysis of angiogenic cytokines of day 18 airways transplanted into WT control or VHL-haplodeficient recipients. b Analysis of angiogenesis-related receptors of day 18 airway allografts (n = 4–6). Data are shown as means ± SEM. *P < 0.05; **P < 0.01, Student’s t test

Xinguo Jiang, et al. J Mol Med (Berl). 2013;91(9):1081-1093.
2.
Fig. 5

Fig. 5. From: Tie2-dependent VHL knockdown promotes airway microvascular regeneration and attenuates invasive growth of Aspergillus fumigatus.

Diminished microvascular perfusion of airways transplanted into recipient with HIF-1α knockout in Tie2 lineage cells. a Representative FITC-lectin microvascular perfusion images at various time points posttransplantation. b Quantification of relative microvascular perfusion (n = 4–6). c Laser Doppler flowmetry analysis showing relative perfusion of tracheal transplants (n = 4–6 per time point). Scale bar, 100 μm (a). Data are shown as means ± SEM. *P < 0.05, Student’s t test

Xinguo Jiang, et al. J Mol Med (Berl). 2013;91(9):1081-1093.
3.
Fig. 4

Fig. 4. From: Tie2-dependent VHL knockdown promotes airway microvascular regeneration and attenuates invasive growth of Aspergillus fumigatus.

Improved vessel function and maturation in airways transplanted into recipients with Tie2+ cell VHL haplodeficiency. a Representative confocal images showing extravasated 50 nm microsphere (red) and microvascular perfusion (green) in normal airways and airways transplanted into control or VHL-haplodeficient recipients at day 28. b Quantification of microsphere extravasation (n = 4–6). c CD31 (marker for ECs) and desmin (marker for pericytes) staining showed more complete pericyte-covered vessels in airways transplanted into VHL-haplodeficient recipients at day 28. d Quantification of pericyte coverage in the microvasculature of transplanted airways (n = 3–5). Scale bars, 100 μm (a), 20 μM (c). Data are shown as means ± SEM, *P < 0.05, Student’s t test

Xinguo Jiang, et al. J Mol Med (Berl). 2013;91(9):1081-1093.
4.
Fig. 1

Fig. 1. From: Tie2-dependent VHL knockdown promotes airway microvascular regeneration and attenuates invasive growth of Aspergillus fumigatus.

Recipient Tie2+ cell VHL haplodeficiency prolongs airway microvascular perfusion and diminishes tissue hypoxia. a Confocal microscopic FITC-lectin perfusion images showing microvascular perfusion of tracheas transplanted into control recipients with normal VHL expression compared with recipients with VHL haplodeficiency in Tie2+ cells. b Quantification of perfused airway microvasculature following transplantation (n = 4–6). c Airway blood perfusion measured by laser Doppler flowmetry in tracheas transplanted into recipients with or without VHL haplodeficiency (n = 4–6 per time point). d Pimonidazole staining (green, red arrows) in transplanted airways in both day 16 and day 28 showing decreased hypoxia in grafts transplanted into VHL-haplodeficient recipients. Scale bars, 100 μm (a and d). Data are shown as means ± SEM. *P < 0.05 at individual time points, Student’s t test

Xinguo Jiang, et al. J Mol Med (Berl). 2013;91(9):1081-1093.
5.
Fig. 2

Fig. 2. From: Tie2-dependent VHL knockdown promotes airway microvascular regeneration and attenuates invasive growth of Aspergillus fumigatus.

VHL-haplodeficient lung endothelial cells exhibit enhanced angiogenic activity and increased resistance to serum deprivation-induced cell death. a Migration of wild type (WT) and VHL-haplodeficient (VHL+/−) lung EC was assessed by scratch wound assay. Representative pictures were taken 6 h after scratch. b Percentage of wound closure was quantified 6 h after scratch at culturing conditions with 0.5 or 5 % FBS. c Network/capillary formation of WT and VHL-haplodeficient ECs was assessed using a Matrigel assay. Representative images were taken 6 h after culturing in 0.5 or 5 % FBS. d Quantification of network formation as measured by total cellular cord length for VHL-haplodeficient ECs in both 0.5 and 5 % FBS conditions. e Cell death assessed by trypan blue assay. Experiments were performed three times. Scale bars, 300 μm (a and c). Data are shown as means ± SEM.*P < 0.05; **P < 0.01, Student’s t test

Xinguo Jiang, et al. J Mol Med (Berl). 2013;91(9):1081-1093.
6.
Fig. 6

Fig. 6. From: Tie2-dependent VHL knockdown promotes airway microvascular regeneration and attenuates invasive growth of Aspergillus fumigatus.

Tie2+ cell VHL haplodeficiency attenuates A. fumigatus invasion. All animals were infected with A. fumigatus on posttransplant day 16 and euthanized on day 18. A semiquantitative scoring system was used to grade the degree of fungal burden (0–4 scale, Supplementary Fig. 3a) and depth of fungal invasion (0–3 scale, Supplementary Fig. 3b). On average, 420 transverse sections or 180 longitudinal sections were evaluated and the section with the highest grade was used for scoring. a Degree of fungal burden, as measured on a semiquantitative scale 0–4, by VHL expression status (n = 6). b Depth of fungal invasion, as measured on a semiquantitative scale by VHL expression status. Mean depth of invasion increased in control compared with VHL-haplodeficient mice (n = 8). Representative GMS sections of fungal invasion (n = 14) (ce). c Longitudinal section of A. fumigatus-infected VHL-haplodeficient animal, displaying a high degree of fungal burden (grade 3) without evidence of epithelial invasion (grade 0) (10× magnification), inset (100× magnification). d Transverse section of A. fumigatus-infected control animal with invasion to the depth of the subepithelial layer (grade 2) (10× magnification), inset (100× magnification). e Longitudinal section of A. fumigatus-infected control animal with invasion to the depth of the cartilaginous ring (grade 3) (10× magnification), inset (40× magnification). C denotes cartilaginous ring, E specifies epithelial layer, SE specifies subepithelial layer, TL denotes tracheal lumen (n = 6–8 animals/group). Red arrows denote fungal hyphae in lower magnification images. Data are shown as means ± SEM. *P < 0.05, nonparametric Kruskal–Wallis test

Xinguo Jiang, et al. J Mol Med (Berl). 2013;91(9):1081-1093.

Display Settings:

Items per page

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk