Results: 5

1.
Figure 5

Figure 5. Validation of microarray expression data.. From: Transcriptome Analysis of Genes Regulated by Cholesterol Loading in Two Strains of Mouse Macrophages Associates Lysosome Pathway and ER Stress Response with Atherosclerosis Susceptibility.

Linear regression analysis of microarray expression and qPCR data for Trib3(A), Abcg1 (B), Atf4 (C) and Cln5 (D) performed in experiment 1 unloaded and loaded AKR and DBA/2 macrophages. Microarray data were not log2 transformed for this analysis.

Stela Z. Berisha, et al. PLoS One. 2013;8(5):e65003.
2.
Figure 3

Figure 3. Examples of transcripts with strain-loading interaction effect in experiment 1 samples.. From: Transcriptome Analysis of Genes Regulated by Cholesterol Loading in Two Strains of Mouse Macrophages Associates Lysosome Pathway and ER Stress Response with Atherosclerosis Susceptibility.

Slamf9 (A), Trib3 (B), and Dner (C) expression in unloaded and loaded macrophages. Values are expressed as mean±SD (N = 4). Different numbers above bars show p<0.01 (A), p<0.05 (B, C) by Newman-Keuls ANOVA posttest, while similar numbers above bars show no significant differences.

Stela Z. Berisha, et al. PLoS One. 2013;8(5):e65003.
3.
Figure 1

Figure 1. Total (A), esterified (B), and free (C) cholesterol mass in unloaded and AcLDL loaded AKR and DBA/2 ApoE−/− macrophages.. From: Transcriptome Analysis of Genes Regulated by Cholesterol Loading in Two Strains of Mouse Macrophages Associates Lysosome Pathway and ER Stress Response with Atherosclerosis Susceptibility.

All data are mean±SD, N = 4 per group using the average of triplicate assays per sample. P-values were calculated by ANOVA with Newman-Keuls posttest, showing only the strain differences after loading. There were no significant strain differences in unloaded cells.

Stela Z. Berisha, et al. PLoS One. 2013;8(5):e65003.
4.
Figure 2

Figure 2. Gene expression and validation of microarray data by quantitative real-time PCR in experiment 1 and 2 macrophages.. From: Transcriptome Analysis of Genes Regulated by Cholesterol Loading in Two Strains of Mouse Macrophages Associates Lysosome Pathway and ER Stress Response with Atherosclerosis Susceptibility.

Abca1 (A) and Abcg1 (B) expression was calculated relative to ubiquitin C (Ubc) gene expression, an endogenous control whose expression remained unchanged under the conditions of experiment. Values are expressed as mean±SD (N = 4). Different numbers above bars show p<0.001 (A) or p<0.05 (B) by Newman-Keuls ANOVA posttest, while similar numbers above bars show no significant differences.

Stela Z. Berisha, et al. PLoS One. 2013;8(5):e65003.
5.
Figure 4

Figure 4. Examples of transcripts with strain-loading interaction effect in experiment 2 samples.. From: Transcriptome Analysis of Genes Regulated by Cholesterol Loading in Two Strains of Mouse Macrophages Associates Lysosome Pathway and ER Stress Response with Atherosclerosis Susceptibility.

Ddit3 (A), Ccr5 (B), and Trib3(C) expression in unloaded and loaded macrophages. Values are expressed as mean±SD (N = 4). Different numbers above bars show p<0.05 (A), p<0.001 (B), or p<0.001 (C) by Newman-Keuls ANOVA posttest, while similar numbers above bars show no significant differences. (D) Proteins isolated from unloaded and cholesterol loaded AKR and DBA/2 BMM were subjected to Western blot analysis, showing TRIB3 band density normalized to GAPDH.

Stela Z. Berisha, et al. PLoS One. 2013;8(5):e65003.

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