Display Settings:

Items per page
We are sorry, but NCBI web applications do not support your browser and may not function properly. More information

Results: 8

1.
Figure 1

Figure 1. Characterization and differentiation of hUC-MSCs.. From: Suppression of Cholangiocarcinoma Cell Growth by Human Umbilical Cord Mesenchymal Stem Cells: A Possible Role of Wnt and Akt Signaling.

A–G, Flow-cytometric analysis of cell surface antigens of hUC-MSC; H, Alizarin red S staining of osteogenic differentiated hUC-MSCs (400×); I, Oil red O staining of adipogenic differentiated hUC-MSCs (400×).

Juan Liu, et al. PLoS One. 2013; 2013;8(4):e62844.
2.
Figure 7

Figure 7. Thermolability of components of conditioned media.. From: Suppression of Cholangiocarcinoma Cell Growth by Human Umbilical Cord Mesenchymal Stem Cells: A Possible Role of Wnt and Akt Signaling.

The conditioned media were heated to various temperatures and cooled to room temperature prior to preparing the 50% conditioned media. When samples were heated to 75°C or above for 5 mins or longer, the growth inhibition decreased from 49.8% to 11.5%.

Juan Liu, et al. PLoS One. 2013; 2013;8(4):e62844.
3.
Figure 8

Figure 8. Proposed model by which hUC-MSC cultures mediates cross-talk between Wnt and Akt signaling in HCCC-9810 cells.. From: Suppression of Cholangiocarcinoma Cell Growth by Human Umbilical Cord Mesenchymal Stem Cells: A Possible Role of Wnt and Akt Signaling.

Inhibitory molecules in hUC-MSC conditioned media mediate Akt phosphorylation in HCCC-9810 cells, thereby decreasing phosphorylation of GSK-3βand increasing its activity. Activation of GSK-3βleads to decreased cellularβ-catenin levels. A decrease in β-catenin translocation to the nucleus to bind TCF4, and decreases the transcription of its specific target genes resulting in apoptosis.

Juan Liu, et al. PLoS One. 2013; 2013;8(4):e62844.
4.
Figure 6

Figure 6. Effect of the GSK-3β inhibitor/activator, and IGF-1 on hUC-MSCs-mediated intrahepatic cholangiocarcinoma cell toxicity.. From: Suppression of Cholangiocarcinoma Cell Growth by Human Umbilical Cord Mesenchymal Stem Cells: A Possible Role of Wnt and Akt Signaling.

HCCC-9810 cells were incubated with the GSK-3β activator SNP, and the GSK-3β inhibitor CHIR99021 as a single treatment or in combination with hUC-MSC conditioned media for 48 h, and cell survival was measured using the MTT assay (A, B). A similar MTT assay was carried out using IGF-1 (C). The expression of β-catenin and phospho-Akt (Ser 437 and Thr308) was measured by immunoblot analysis.

Juan Liu, et al. PLoS One. 2013; 2013;8(4):e62844.
5.
Figure 5

Figure 5. Effect of conditioned media from hUC-MSCs on the Wnt signaling pathway of HCCC-9810 cells.. From: Suppression of Cholangiocarcinoma Cell Growth by Human Umbilical Cord Mesenchymal Stem Cells: A Possible Role of Wnt and Akt Signaling.

Immunoblot analysis showed that the treatment of HCCC-9810 cells with 50% conditioned media from hUC-MSCs cultures led to the down-regulation of β-catenin, c-Myc, and cyclin-D1 expression, while the levels of these proteins in control groups was unchanged (A). After treatment for 48 h, 50% conditioned media from hUC-MSCs cultures prevented nuclear translocation of β-catenin in HCCC-9810 cells (B). Results shown are representative of three independent experiments.

Juan Liu, et al. PLoS One. 2013; 2013;8(4):e62844.
6.
Figure 3

Figure 3. Induction of apoptosis by hUC-MSCs conditioned media in HCCC-9810 cells.. From: Suppression of Cholangiocarcinoma Cell Growth by Human Umbilical Cord Mesenchymal Stem Cells: A Possible Role of Wnt and Akt Signaling.

Cells were treated with 50% conditioned media from hUC-MSCs or HUVEC or without conditioned media (MOCK) for 48 h. The apoptotic tumor cells stained with Hoechst 33258 are designated by the arrow (A, 400×). The histogram shows the rates of Hoechst 33258-positive cells (P<0.05, B). Cells were incubated with 50% hUC-MSC conditioned media for 48 h. DNA fragmentation was detected by 1.5% agarose gel containing ethidium bromide. A representative blot was shown from three independent experiments (C).

Juan Liu, et al. PLoS One. 2013; 2013;8(4):e62844.
7.
Figure 4

Figure 4. Effect of conditioned media from hUC-MSCs on the Akt signaling pathway.. From: Suppression of Cholangiocarcinoma Cell Growth by Human Umbilical Cord Mesenchymal Stem Cells: A Possible Role of Wnt and Akt Signaling.

Immunoblot analysis showed that treatment of HCCC-9810 cells with 50% conditioned media from hUC-MSCs cultures led to the down-regulation of p-PI3K, p-PDK1Ser241, p-AktThr308, p-AktsSer473 and p- GSK-3βSer9 expression. However, this did not occur in HCCC-9810 cells with 50% conditioned media from HUVEC cultures. There were substantial differences in the phospho- to total Akt and Gsk-3β ratios between the treated group and control group (A). After treatment for 48 h with 50% conditioned media from hUC-MSCs cultures, the expression of phospho-Akt (B, 200×) and phospho-GSK-3β (C, 200×) in HCCC-9810 cells assessed by immunofluorescence. Results shown are representative of three independent experiments.

Juan Liu, et al. PLoS One. 2013; 2013;8(4):e62844.
8.
Figure 2

Figure 2. The inhibitory effect of hUC-MSCs on intrahepatic cholangiocarcinoma.. From: Suppression of Cholangiocarcinoma Cell Growth by Human Umbilical Cord Mesenchymal Stem Cells: A Possible Role of Wnt and Akt Signaling.

HCCC-9810 and HUVEC controls cells were co-cultured with hMSCs for 48 h, after which cells were harvested for counting. The number of cells is represented as the mean±SD of three independent experiments. Results are shown as the percentage of cell number compared with the number of HUVEC control cells. Compared with that of the control, the cell proliferation rate decreased 38.58% (**P<0.05; A). Colony-forming assays showed that there were significantly fewer colony-forming units of HCCC-9810 cells treated with conditioned media from hUC-MSCs than that of control cells. The mean numbers of colony-forming units in the hUC-MSC media treatment group, and the HUVEC media control group were 14.7 and 35.7, respectively (P<0.01, B). HCCC-9810 cells were treated with various concentrations of conditioned media from hUC-MSCs (10%,25%,50% and 75%) for 12, 24 and 48 h. MTT assays showed that the inhibitory effects of test groups were significantly higher than that of the HUVEC control (C). The inhibition rate increased to 52.84% in the test group, while it only reached 6.87% in the controls after treatment for 48 h. This growth inhibition was not due to a lack of required nutrients due to consumption during preparation of the conditioned media since cell proliferation did not change when treated with 50% hUC-MSC conditioned media together with increasing concentrations of fetal calf serum (10%, 25%, and 50%) (D). The inhibitory effect was not limited to HCCC-9810 cells (E). There was a similar effect with Eca-109 cells, but not with MCF-7 or L-02 cells. When HCCC-9810 cells were co-injected with hUC-MSCs, smaller tumors formed (arrow) compared to those of HUVEC controls, and the formed tumors shrank in size after injection of conditioned media from hUC-MSC (F).

Juan Liu, et al. PLoS One. 2013; 2013;8(4):e62844.

Display Settings:

Items per page

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk