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Results: 6

1.
Fig. 5

Fig. 5. From: Development and characterization of two porcine monocyte-derived macrophage cell lines.

Cytokine expression measured by RT-qPCR and normalized against the average of three housekeeping genes.

Carol G. Chitko-McKown, et al. Results Immunol. 2013;3:26-32.
2.
Fig. 4

Fig. 4. From: Development and characterization of two porcine monocyte-derived macrophage cell lines.

Phagocytosis of latex microspheres. Phagocytosis was measured by the uptake of fluorescent microspheres as described in Section 2. Data is presented as % cells ingesting microspheres at the indicated time-points.

Carol G. Chitko-McKown, et al. Results Immunol. 2013;3:26-32.
3.
Fig. 2

Fig. 2. From: Development and characterization of two porcine monocyte-derived macrophage cell lines.

Karyotypes of cell lines. A) CΔ2+ and B) CΔ2− representative karyotypes are arranged according to standard nomenclature. Note derivative chromosomes SSC 8 and SSC 16 in A.

Carol G. Chitko-McKown, et al. Results Immunol. 2013;3:26-32.
4.
Fig. 1

Fig. 1. From: Development and characterization of two porcine monocyte-derived macrophage cell lines.

Histologic staining of cell lines. (A) Phase-contrast photomicrographs of CΔ2+ and CΔ2− cell lines. (B) Cytocentrifuged preparations of control and LPS-treated cell lines were fixed and stained with Hema 3 differential stain, and for (C) leukocyte peroxidase and α-naphthyl esterase activity as per the manufacturers’ directions.

Carol G. Chitko-McKown, et al. Results Immunol. 2013;3:26-32.
5.
Fig. 3

Fig. 3. From: Development and characterization of two porcine monocyte-derived macrophage cell lines.

Representative flow cytometry histogram. For each antibody, cells stained with isotype control were estimated by quantifying how many cells were localized under gate 1 (G1, Top Panel) compared to how many cells were localized under gate 2 (G2, Middle Panel). Overlay of the two histograms is shown for comparative purposes (Bottom Panel).

Carol G. Chitko-McKown, et al. Results Immunol. 2013;3:26-32.
6.
Fig. 6

Fig. 6. From: Development and characterization of two porcine monocyte-derived macrophage cell lines.

PCR test to measure contamination of cell lines with BVDV. Aliquots of CΔ2− and CΔ2+ lysates were mixed 1:1 with Minimum Essential Medium (MEM) and inoculated onto bovine turbinate (BT) cells that had been seeded into a 24-well plate. After 14 days of incubation at 37 °C, the BT cell lysates were tested by PCR for propagation of BVDV.

Carol G. Chitko-McKown, et al. Results Immunol. 2013;3:26-32.

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