Display Settings:

Items per page

Results: 8

1.
Fig. 6.

Fig. 6. From: Hyperoxia-induced hypertrophy and ion channel remodeling in left ventricle.

Intracellular TNF-α expression in hyperoxic mice. The intracellular TNF-α expression was measured using ELISA, and values were expressed in picograms of TNF-α per microgram of total protein. The values on y-axis are means ± SE (n = 3). The numbers above the bar represent the fold values compared with normoxia group. *P ≤ 0.05.

Siva K. Panguluri, et al. Am J Physiol Heart Circ Physiol. 2013 June 15;304(12):H1651-H1661.
2.
Fig. 3.

Fig. 3. From: Hyperoxia-induced hypertrophy and ion channel remodeling in left ventricle.

Gene expression profile of transcriptional factors. qRT-PCR expression profile of transcriptional factors such as Mef2c, GATA4, NFκB1, PPARγ, Srf, and Hif1α. Values shown on y-axis are means ± SE (n = 3) and are normalized with housekeeping gene HPRT expression level. The numbers above the bar represents the fold values compared with normoxia group, and all the genes are with P ≤ 0.05.

Siva K. Panguluri, et al. Am J Physiol Heart Circ Physiol. 2013 June 15;304(12):H1651-H1661.
3.
Fig. 7.

Fig. 7. From: Hyperoxia-induced hypertrophy and ion channel remodeling in left ventricle.

Interacting gene network of differentially expressed genes in left ventricle of hyperoxic mice. Genes and their expression levels from qRT-PCR data with P ≤ 0.05 were considered for network analysis using GeneGo software. The software uses the curated publications to build the relations between the genes. Upregulated genes are marked with red circles; downregulated with blue circles. The checkerboard color indicates mixed expression for the gene between files or between multiple tags for the same gene.

Siva K. Panguluri, et al. Am J Physiol Heart Circ Physiol. 2013 June 15;304(12):H1651-H1661.
4.
Fig. 8.

Fig. 8. From: Hyperoxia-induced hypertrophy and ion channel remodeling in left ventricle.

In silico analysis of the reported genes. Top 5 cellular pathways affected by hyperoxia treatment. A: top ion channels and functional target functions affected by hyperoxia treatment, B: signaling and pathological targets affected by hyperoxia, C: toxicology networks affected by hyperoxia treatment. The values on y-axis show the top predicted hits depicted with scale of negative log (−log) of false discovery rate (FDR) corrected P values.

Siva K. Panguluri, et al. Am J Physiol Heart Circ Physiol. 2013 June 15;304(12):H1651-H1661.
5.
Fig. 1.

Fig. 1. From: Hyperoxia-induced hypertrophy and ion channel remodeling in left ventricle.

Physical parameters of heart. A: the normalized body weights of normoxia and hyperoxia mice were plotted as means ± SE (n = 14). *P ≤ 0.05. B: whole heart. Representative pictorial depiction is provided for the difference in size of the whole heart between hyperoxia and normoxia groups. C: normalized heart weight of hyperoxia- and normoxia-treated mice expressed in g/cm on y-axis. All the values plotted are means ± SE (n = 13 for normoxic and n = 9 for hyperoxic). *P ≤ 0.05. D: hematoxylin and eosin (H and E) staining of normoxia- and hyperoxia-treated hearts. Data represented are means ± SE (n = 3). *P ≤ 0.05.

Siva K. Panguluri, et al. Am J Physiol Heart Circ Physiol. 2013 June 15;304(12):H1651-H1661.
6.
Fig. 5.

Fig. 5. From: Hyperoxia-induced hypertrophy and ion channel remodeling in left ventricle.

Western analysis of ion channels, transcriptional factors, and hypertrophic markers in right ventricle (RV). Western blot analysis from hyperoxic or normoxic group with anti-Kv4.2, anti-Irx5, anti-Gapdh, anti-Kv1.4, anti-MHC7, anti-NFκB, anti-KChIP2 and anti-phosphorylated NFκB antibodies (A); lane M represents prestained protein ladder. The band intensities from all the blots were measured using ImageJ software and were normalized with their corresponding Gapdh band intensities (B). The values shown on y-axis are means ± SE (n = 3) of normalized band intensities. The numbers above the bar represents the fold values compared with normoxia group. *P ≤ 0.05.

Siva K. Panguluri, et al. Am J Physiol Heart Circ Physiol. 2013 June 15;304(12):H1651-H1661.
7.
Fig. 2.

Fig. 2. From: Hyperoxia-induced hypertrophy and ion channel remodeling in left ventricle.

Transcriptional changes in hypertrophic and ion channel gene expression. Left ventricle of normoxic and hyperoxic groups were used for real-time qRT-PCR with hypertrophic markers MHC-6 or MHC-α and MHC-7 or MHC-β (A) and ion channel genes including Kv4.2, Kv2.1, Kv4.3, Kcnj2, Scn5a and their related proteins such as KChIP2, Irx5 and Gja1 (B). Values shown on y-axis are means ± SE (n = 3) and are normalized with housekeeping gene HPRT expression level. The numbers above the bar represent the fold values compared with normoxia group and all the genes are with P ≤ 0.05.

Siva K. Panguluri, et al. Am J Physiol Heart Circ Physiol. 2013 June 15;304(12):H1651-H1661.
8.
Fig. 4.

Fig. 4. From: Hyperoxia-induced hypertrophy and ion channel remodeling in left ventricle.

Translational changes in ion channels, transcriptional factors and hypertrophic markers in left ventricle (LV). Western blot analysis from hyperoxic or normoxic group developed with anti-Kv4.2, anti-Irx5, anti-Gapdh, anti-Kv1.4, anti-MHC7, anti-NFκB, anti-KChIP2 and anti-phosphorylated NFκB antibodies (A); lane M represents prestained protein ladder. The band intensities from all the blots were measured using ImageJ software and were normalized with their corresponding Gapdh band intensities (B). Values shown on y-axis are means ± SE (n = 3) of normalized band intensities. The numbers above the bar represent the fold values compared with normoxia group. *P ≤ 0.05.

Siva K. Panguluri, et al. Am J Physiol Heart Circ Physiol. 2013 June 15;304(12):H1651-H1661.

Display Settings:

Items per page

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk