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1.
Figure 6

Figure 6. Esophageal eosinophil level in an allergen-induced experimental eosinophil esophagitis model in miR-21+/+ and miR-21−/− mice.. From: Targeted Ablation of miR-21 Decreases Murine Eosinophil Progenitor Cell Growth.

Eosinophil levels in the esophagus were determined by morphometric analysis following anti-MBP staining. N = 5–6 mice per group. Data are represented as mean ± S.E.M. Data are representative of 2 independent experiments.

Thomas X. Lu, et al. PLoS One. 2013;8(3):e59397.
2.
Figure 3

Figure 3. Levels of apoptosis in the eosinophil progenitor culture from the miR-21+/+ and miR-21−/− mice.. From: Targeted Ablation of miR-21 Decreases Murine Eosinophil Progenitor Cell Growth.

Levels of Annexin V and 7AAD staining during eosinophil differentiation culture as determined by FACS. The viable cells are AnnexinV 7AAD. The early apoptotic cells are AnnexinV+ 7AAD. The late apoptotic cells are AnnexinV+ 7AAD+. Data are representative of 3 independent experiments.

Thomas X. Lu, et al. PLoS One. 2013;8(3):e59397.
3.
Figure 1

Figure 1. MiR-21 is induced during eosinophil differentiation.. From: Targeted Ablation of miR-21 Decreases Murine Eosinophil Progenitor Cell Growth.

(A) Purity of cultured eosinophils at day 14. Eosinophils are identified as CCR3+Siglec-F+ cells. (B) Levels of miR-21 during the eosinophil differentiation culture determined by qPCR normalized to U6 small nuclear RNA. N = 3 per group; data are represented as mean ± S.E.M. Data are representative of 3 independent experiments.

Thomas X. Lu, et al. PLoS One. 2013;8(3):e59397.
4.
Figure 4

Figure 4. Blood eosinophil percentage and bone marrow eosinophil colony forming unit capacity in the miR-21+/+ and miR-21−/− mice.. From: Targeted Ablation of miR-21 Decreases Murine Eosinophil Progenitor Cell Growth.

(A) Blood eosinophil percentage from miR-21+/+ and miR-21−/− mice determined by FACS staining for CCR3+Siglec-F+ cells; n = 9–10 mice per group. (B) Bone marrow eosinophil colony-forming unit (CFU-Eos) and (C) neutrophil colony-forming unit (CFU-G) capacity from miR-21+/+ and miR-21−/− mice; n = 4 per group. Data are represented as mean ± S.E.M. Data are representative of 3 independent experiments.

Thomas X. Lu, et al. PLoS One. 2013;8(3):e59397.
5.
Figure 2

Figure 2. Growth of eosinophil progenitor cells from miR-21−/− mice and miR-21+/+ controls during the ex vivo eosinophil culture.. From: Targeted Ablation of miR-21 Decreases Murine Eosinophil Progenitor Cell Growth.

Total cell number of (A) eosinophil and (B) neutrophil cultures derived from miR-21+/+ and miR-21−/− mice is shown. N = 6 per group; data are represented as mean ± S.E.M. **: p<0.01. (C) Morphology of miR-21+/+ and miR-21−/− cultured eosinophils at day 12 determined by Diff-Quik staining. Data are representative of 3 independent experiments for panels A and C; data are representative of 2 independent experiments for panel B.

Thomas X. Lu, et al. PLoS One. 2013;8(3):e59397.
6.
Figure 5

Figure 5. Differentially regulated genes between miR-21+/+ and miR-21−/− eosinophil progenitor cultures at day 8 and day 12.. From: Targeted Ablation of miR-21 Decreases Murine Eosinophil Progenitor Cell Growth.

(A) Heat map of differentially regulated genes at day 8 of the eosinophil differentiation culture. Red: up-regulated in miR-21−/− eosinophil progenitor culture compared to miR-21+/+ eosinophil progenitor culture. (B) Heat map of differentially regulated genes at day 12 of the eosinophil differentiation culture. Red: up-regulated in miR-21−/− eosinophil progenitor culture compared to miR-21+/+ eosinophil progenitor culture; blue: down-regulated in miR-21−/− eosinophil progenitor culture compared to miR-21+/+ eosinophil progenitor culture. (C) Quantitative RT-PCR verification of a selected set of differentially expressed genes between miR-21+/+ and miR-21−/− eosinophil progenitor cultures. (D) Western blot showing levels of PSRC1 in miR-21+/+ and miR-21−/− eosinophil progenitor cultures at day 8 and day 12. GAPDH is shown as a loading control. (E) Functional enrichment analysis of differentially regulated genes in the eosinophil progenitor cultures at day 12. The networks are shown as Cytoscape graph networks generated from ToppCluster network analysis.

Thomas X. Lu, et al. PLoS One. 2013;8(3):e59397.

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