Results: 4

1.
Figure 4

Figure 4. Comparison of splenic white pulp in Sharpincpdm mutant and control mice.. From: Loss of Function of the Mouse Sharpin Gene Results in Peyer’s Patch Regression.

The white pulp of control (A) mice is discrete and well-organized. B and T cell regions of the Sharpincpdm mutant (B) spleen are smaller, organization is poor, and splenomegaly is common (10×). Images represent at least 3 mice per group examined at each time point. Green–B220+ B cells, Red–CD3+ T cells, Blue–DAPI (nuclear) counterstain. IFA.

Rosemarie Seymour, et al. PLoS One. 2013;8(2):e55224.
2.
Figure 2

Figure 2. Regression of lymphoid tissue in Sharpincpdm mutant mice.. From: Loss of Function of the Mouse Sharpin Gene Results in Peyer’s Patch Regression.

Histologic sections of Peyer’s patches in control mice (A) and residual lymphoid tissue in Sharpincpdm mutant mice (B) at 1 to 5 weeks of age are shown. Images represent at least 3 mice per group examined at each time point. Some variability is observed in the cellular composition of individual lymphoid tissue remnants in mutant mice, however eosinophilic/granulocytic and stromal effacement is common. Lymphoid patches were not found in some mutant mice at 4 to 5 weeks using this method. H&E.

Rosemarie Seymour, et al. PLoS One. 2013;8(2):e55224.
3.
Figure 3

Figure 3. B220+ B and CD3+ T lymphocyte organization in Peyer’s patches (10X).. From: Loss of Function of the Mouse Sharpin Gene Results in Peyer’s Patch Regression.

Images represent at least 3 control mice (A) and Sharpincpdm mutant mice (B) examined at each time point. Lymphoid patches were not found in the small intestines of some 4- and 5-week old Sharpincpdm mutant mice using this method. (C) Longitudinal section of a Peyer’s patch from a control mouse showing normal segregation of B and T cell zones. Green–B220+ B cells, Red–CD3+ T cells, Blue–DAPI (nuclear) counterstain. IFA.

Rosemarie Seymour, et al. PLoS One. 2013;8(2):e55224.
4.
Figure 1

Figure 1. Lack of compartmentalized Peyer’s patches (PP) in Sharpincpdm mutant mice.. From: Loss of Function of the Mouse Sharpin Gene Results in Peyer’s Patch Regression.

(A) Intercellular adhesion molecule (ICAM1) labeling in the small intestine of 1 day old mice. Numerous ICAM1+ patches are present in the Sharpincpdm mutant mice (left), however they lack the distinct compartmentalization seen in most ICAM1+ patches of control (+/?) mice (right). (B) Mean ICAM1+ counts for a litter of 1 day old Sharpincpdm mutant (N = 3) and control (+/?) mice (N = 8). Bars indicate standard error of the mean. (C) Anti-CD45RB (B220) labeling in the small intestine of mutant Sharpincpdm (top panel) and control (bottom panel) mice at (left) 4 days, (center) 1 week, and (right) 2 weeks of age. Images are representative of 3 to 4 mice per group; PP of control mice appear compartmentalized and follicular structures are evident, and overall size of PP subjectively increase with age, while those of mutant mice do not show these features. WMI.

Rosemarie Seymour, et al. PLoS One. 2013;8(2):e55224.

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk